LOCUS       40924_33128        4366 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for monitoring NF-kappa-B-mediated signal transduction using 
            secreted Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    46..85
                     /label=NFkB enhancer element
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B RE"
                     /note="NF-kappa-B response element (4 copies)"
     promoter        98..129
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             213..869
                     /codon_start=1
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
                     /translation="MDIKVVFTLVFSALVQAKSTEFDPNIDIVGLEGKFGITNLETDLF
                     TIWETMEVMIKADIADTDRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTR
                     GCLICLSKIKCTAKMKVYIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQF
                     IAQVDRCASCTTGCLKGLANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     polyA_signal    994..1115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1122..1577)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1604..1708
                     /label=AmpR promoter
     promoter        1710..2067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2102..2893
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3128..3175
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3504..4092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4154..4202
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4216..4307
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttgggaa tttccgggaa
       61 tttccgggaa tttccgggaa tttccagatc tagactctag agggtatata atggaagctc
      121 gaattccagc ttggcattcc ggtactgttg gtaaaaagct tcgaattctg cagtcgacgg
      181 taccgcgggc ccgggatcca ccggtcgcca ccatggacat caaggtggtg ttcaccctgg
      241 tgttcagcgc cctggtgcag gccaagagca ccgagttcga ccccaacatc gacatcgtgg
      301 gcctggaagg caagttcggc atcaccaacc tggaaaccga cctgttcacc atctgggaga
      361 ccatggaagt gatgatcaag gccgacatcg ccgacaccga ccgggccagc aacttcgtgg
      421 ccaccgagac cgacgccaac cggggcaaga tgcccggcaa gaagctgccc ctggccgtca
      481 tcatggaaat ggaagccaac gccttcaagg ccggctgcac ccggggctgc ctgatctgcc
      541 tgagcaagat caagtgcacc gccaagatga aggtgtacat ccccggcagg tgccacgact
      601 acggcggcga caagaaaacc ggccaggccg gcatcgtggg cgccatcgtg gacatccccg
      661 agatcagcgg cttcaaagaa atggccccca tggaacagtt catcgcccag gtggacagat
      721 gcgccagctg caccaccggc tgcctgaagg gcctggccaa cgtgaagtgc agcgagctgc
      781 tgaagaagtg gctgcccgac cgctgcgcca gcttcgccga caagatccag aaagaggtgc
      841 acaacatcaa gggcatggcc ggcgacaggt gagcggccgc gactctagat cataatcagc
      901 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
      961 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     1021 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1081 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     1141 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     1201 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     1261 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     1321 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     1381 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     1441 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     1501 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     1561 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     1621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1681 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     1741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     1861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     1921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     1981 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2041 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     2101 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     2161 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     2221 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     2281 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     2341 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     2401 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     2461 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     2521 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     2581 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     2641 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     2701 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     2761 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     2821 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     2881 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     2941 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3001 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3061 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     3121 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     3181 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     3241 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     3301 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     3361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4141 tttgctcaca tgtaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     4201 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     4261 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
