LOCUS       V004344                 4097 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004344
VERSION     V004344
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
            Muller F, Bacher A, Fischer M.
  TITLE     (13)C Isotopologue editing of FMN bound to phototropin domains
  JOURNAL   FEBS J. 274 (22), 5876-5890 (2007)
   PUBMED   17944933
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Fischer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Chemistry, University of Hamburg,
            Grindelallee 117, Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 4097)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
            date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117,
            Hamburg 20146, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     misc_feature    511..816
                     /note="Region: Adiantum capillus-veneris phototropin PHY3
                     Lov2 domain"
     terminator      839..933
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1705..1791
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1947..2087
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2273..2861)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3035..3892)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3893..3997)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgggt
      121 aacagagcat tcaaatcaca tcacggtcac tttttaagcg ctgaaggcga agctgtaaag
      181 actcaccacg gtcatcatga tcatcacacc catttccacg ttgaaaacca tggtggtaaa
      241 gttgcattaa agacccattg cggtaaatac ctttcaattg gtgatcataa acaagtttac
      301 ctctcacacc acttacacgg tgaccactca ctcttccact tagaacatca tggcggtaaa
      361 gtctcaatca aaggtcatca ccaccactac atttccgctg atcatcatgg tcatgtttca
      421 accaaagaac accacgatca cgacaccacc tttgaagaaa ttattattgg atccggtctg
      481 gtaccacgcg gtgagcgtat cggtaagtct ttcgtgatca cagatcctcg tttaccagac
      541 aaccctatca tcttcgcgag tgaccggttt ctggagctca cggagtatac acgtgaggaa
      601 gtcctaggta acaactgccg tttcctgcag ggccgcggta ctgatcgtaa agcagtgcag
      661 ctgatccgtg atgcagtcaa ggagcagcgc gatgtcactg tacaggtttt aaactataca
      721 aaaggtgggc gcgccttttg gaatctcttc catcttcaag tcatgcgtga tgaaaatggc
      781 gacgtccaat atttcatcgg agttcagcag gagatgtaag cttaattagc tgagcttgga
      841 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
      901 ggttgccgcc gggcgttttt tattggtgag aatccaagct agcttggcga gattttcagg
      961 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     1021 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     1081 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     1141 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aatttcgtat
     1201 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1261 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     1321 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1381 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     1441 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgcatgg
     1501 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     1561 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     1621 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctgggg
     1681 taatgactct ctagcttgag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1741 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgctc
     1801 tagagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     1861 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     1921 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     1981 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     2041 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     2101 cctcgctcac tgactcgctg cgctcggtct gtcggctgcg gcgagcggta tcagctcact
     2161 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2701 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2941 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3001 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3061 tctcagcgat ctgtctattt cgttcatcca tagctgcctg actccccgtc gtgtagataa
     3121 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3181 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3241 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3301 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3361 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3421 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3481 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3541 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3601 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3661 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3721 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3781 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3841 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3901 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3961 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4021 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4081 ggccctttcg tcttcac
//
