LOCUS       V004345                 4259 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004345
VERSION     V004345
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Eisenreich W, Joshi M, Illarionov B, Richter G, Romisch-Margl W,
            Muller F, Bacher A, Fischer M.
  TITLE     (13)C Isotopologue editing of FMN bound to phototropin domains
  JOURNAL   FEBS J. 274 (22), 5876-5890 (2007)
   PUBMED   17944933
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Fischer M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Chemistry, University of Hamburg,
            Grindelallee 117, Hamburg 20146, Germany
REFERENCE   3  (bases 1 to 4259)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4259)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
            date: "2007"; volume: "274"; issue: "22"; pages: "5876-5890"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2007) Chemistry, University of Hamburg, Grindelallee 117,
            Hamburg 20146, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4259
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             115..468
                     /gene="hatA"
                     /label="Hisactophilin-1"
                     /note="Hisactophilin-1 from Dictyostelium discoideum.
                     Accession#: P13231"
     CDS             487..504
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    505..978
                     /note="Region: Avena sativa phototropin PHY3 fusion protein
                     Lov2 domain"
     terminator      1001..1095
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      1867..1953
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2109..2249
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2435..3023)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3197..4054)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4055..4159)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aaccatgggt
      121 aacagagcat tcaaatcaca tcacggtcac tttttaagcg ctgaaggcga agctgtaaag
      181 actcaccacg gtcatcatga tcatcacacc catttccacg ttgaaaacca tggtggtaaa
      241 gttgcattaa agacccattc cggtaaatac ctttcaattg gtgatcataa acaagtttac
      301 ctctcacacc acttacacgg tgaccactca ctcttccact tagaacatca tggcggtaaa
      361 gtctcaatca aaggtcatca ccaccactac atttccgctg atcatcatgg tcatgtttca
      421 accaaagaac accacgatca cgacaccacc tttgaagaaa ttattattgg ttccgcggca
      481 tctggtctgg tgccacgcgg atccgaattt cttgctacta cacttgaacg tattgagaag
      541 aactttgtca ttactgaccc acgtttgcca gataatccca ttatcttcgc gtccgatagt
      601 ttcttgcagt tgacagaata ttcgcgagaa gaaattctgg gtcgtaactg ccgttttctt
      661 caaggtcctg aaaccgatcg cgcgacagtg cgcaaaattc gtgatgccat cgataaccaa
      721 acagaggtca ctgtacagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc
      781 tttcacttgc agcctatgcg tgatcagaag ggtgatgtcc agtactttat tggtgtccag
      841 ttggatggta ccgaacatgt ccgtgatgcg gccgagcgtg agggtgtcat gctgattaag
      901 aaaactgcag aaaatattga tgaggcggca aaagaacttc cagatgctaa tctgcgtcca
      961 gaggatttgt gggctaacta agcttaatta gctgagcttg gactcctgtt gatagatcca
     1021 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     1081 tttattggtg agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat
     1141 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     1201 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     1261 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1321 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1381 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1441 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1501 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1561 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1621 caatatggac aacttcttcg cccccgtttt caccatgcat gggcaaatat tatacgcaag
     1681 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc
     1741 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     1801 aattttttta aggcagttat tggtgccctt aaacgcctgg ggtaatgact ctctagcttg
     1861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1921 ttgtcggtga acgctctcct gagtaggaca aatccgccgc tctagagctg cctcgcgcgt
     1981 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2041 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2101 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     2161 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     2221 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     2281 tgcgctcggt ctgtcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3241 ttcgttcatc catagctgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4201 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcac
//
