LOCUS       62056_17855        2832 bp DNA     circular SYN 10-JUN-2019
DEFINITION  Expression vector pNC-UC, complete sequence.
ACCESSION   MK720605
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Yan P, Zeng Y, Shen W, Tuo D, Zhou P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2019) Institute of Tropical Bioscience and 
            Biotechnology, Chinese Academy of Tropical Agriculture Sciences, 
            Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2019) Institute of Tropical Bioscience and Biotechnology, 
            Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, 
            Haikou, Hainan 571101, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        181..283
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             314..616
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     rep_origin      complement(951..1539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1713..2570)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2571..2675)
                     /label=AmpR promoter
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agacctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2701 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2761 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2821 gagacggtca ca
//
