LOCUS       62056_17830        5291 bp DNA     circular SYN 04-SEP-2019
DEFINITION  Plant binary expression vector pNC-Green0029, complete sequence.
ACCESSION   MK896902
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Institute of Tropical Bioscience and 
            Biotechnology, Chinese Academy of Tropical Agriculture Sciences, 
            Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2019) Institute of Tropical Bioscience and Biotechnology, 
            Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, 
            Haikou, Hainan 571101, China"
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        181..283
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             314..616
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(690..706)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(743..761)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(782..798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(806..822)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(830..861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(876..897)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    1136..1160
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1251..1839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1943..2755)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3046..3481
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3614..3636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(3660..3911)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3954..4745)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4779..4958)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     5205..5221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5228..5246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5272..5288
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agacccctgc agcccggggg atccactagt tctagagcgg ccgccaccgc
      721 ggtggagctc cagcttttgt tccctttagt gagggttaat tccgagcttg gcgtaatcat
      781 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      841 ccggaaghca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      901 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      961 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1021 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1081 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca
     1141 ggatatattg gcgggtaaac taagtcgctg tatgtgtttg tttgagatct catgtgagca
     1201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1681 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catggttaca
     1921 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     1981 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     2041 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     2101 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     2161 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     2221 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     2281 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     2341 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     2401 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
     2461 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     2521 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     2581 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat ccatagattg
     2641 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     2701 tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac
     2761 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     2821 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     2881 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     2941 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     3001 gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg
     3061 ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga
     3121 aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta
     3181 tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct
     3241 aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca
     3301 gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca
     3361 attagctttt caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa
     3421 tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg
     3481 ccagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat aaattttttt
     3541 ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat ttccgatccc
     3601 cggaattaga tcttggcagg atatattgtg gtgtaacgtt atcagcttgc atgccggtcg
     3661 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     3721 gttttctatc gcgtattaaa tgtataattg cgggactcta atcaaaaaac ccatctcata
     3781 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     3841 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     3901 tttgaacgat ctgcttgact ctagctagag tccgaacccc agagtcccgc tcagaagaac
     3961 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc
     4021 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac
     4081 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag
     4141 cggccatttt ccaccatgat attcggcaag caggcatcgc cctgggtcac gacgagatcc
     4201 tcgccgtcgg gcatccgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga
     4261 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtcctcgc
     4321 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc
     4381 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg
     4441 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg
     4501 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     4561 tcttggagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     4621 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     4681 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     4741 atcatgcctc gatcgagttg agagtgaata tgagactcta attggatacc gaggggaatt
     4801 tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
     4861 gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
     4921 aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg
     4981 gcttgtcccg cgtcatcggc gggggtcata acgtgactcc cttaattctc atgtatcgat
     5041 aacattaacg tttacaattt cgcgccattc gccattcagg ctgcgcaact gttgggaagg
     5101 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     5161 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     5221 tgaattgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga
     5281 cggtatcgat a
//
