LOCUS       62056_17785        6987 bp DNA     circular SYN 04-SEP-2019
DEFINITION  Plant binary expression vector pNC-BiFC-Enn, complete sequence.
ACCESSION   MK896895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Yan P, Zeng Y, Shen W, Tuo D, Li X, Zhou P.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Institute of Tropical Bioscience and 
            Biotechnology, Chinese Academy of Tropical Agriculture Sciences, 
            Xueyuan Road, Haikou, Hainan 571101, China
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2019) Institute of Tropical Bioscience and Biotechnology, 
            Chinese Academy of Tropical Agriculture Sciences, Xueyuan Road, 
            Haikou, Hainan 571101, China"
FEATURES             Location/Qualifiers
     source          1..6987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        118..789
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      799..929
                     /label=translational enhancer
                     /note="translational enhancer"
                     /regulatory_class="enhancer"
     CDS             932..1450
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     promoter        1650..1752
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1783..2085
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      2155..2365
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2387..2403)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2440..2458)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2479..2495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2503..2519)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2527..2558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2573..2594)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    2833..2857
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3640..4452)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4743..5178
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    5311..5333
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(5357..5608)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5651..6442)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(6476..6655)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6925..6943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6969..6985
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgttg taaaacgacg gccagtgccg gcgcgccacc ggtcaacatg tggagcacga
       61 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga
      121 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg
      181 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga
      241 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc
      301 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
      361 ttgatgtgat aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      421 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      481 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      541 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      601 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      661 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
      721 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
      781 ggagaggacg tcgagagttc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
      841 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
      901 tgaaaatttt caccatttac gaacgatagc catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1141 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1381 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1441 caacatcgag gactccggac tcagatctca gtggtctctg tccagtcctg gcctcgtcgg
     1501 ccattctcga ctaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     1561 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     1621 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     1681 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1741 aaggaagcta aacttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     1801 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     1861 acgcctgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     1921 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     1981 gatatggcca gtgtgccggt atccgttatc ggggaagaag tggctgatct cagccaccgc
     2041 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2101 cttatacaca gggccagtct ggccacttgt ggtctgctga gaccctgatc tagagtccgc
     2161 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     2221 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     2281 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     2341 attcctaaaa ccaaaatcca gtgacgcgaa ttcctgcagc ccgggggatc cactagttct
     2401 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattcc
     2461 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2521 tccacacaac atacgagccg gaaghcataa agtgtaaagc ctggggtgcc taatgagtga
     2581 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     2701 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2761 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2821 acatgaaggc cttgacagga tatattggcg ggtaaactaa gtcgctgtat gtgtttgttt
     2881 gagatctcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3361 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3601 ttttggtcat ggttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
     3661 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     3721 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     3781 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     3841 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     3901 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     3961 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     4021 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     4081 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     4141 tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     4201 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     4261 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     4321 atacaatcca tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     4381 atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg
     4441 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     4501 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     4561 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     4621 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa
     4681 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
     4741 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
     4801 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
     4861 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
     4921 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
     4981 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
     5041 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
     5101 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
     5161 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
     5221 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
     5281 tctggatttc cgatccccgg aattagatct tggcaggata tattgtggtg taacgttatc
     5341 agcttgcatg ccggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     5401 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     5461 aaaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt
     5521 aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga
     5581 aactttattg ccaaatgttt gaacgatctg cttgactcta gctagagtcc gaaccccaga
     5641 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     5701 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     5761 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     5821 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccct
     5881 gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg
     5941 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     6001 tccgagtacg tcctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     6061 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     6121 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     6181 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     6241 atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg gtcttgacaa
     6301 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     6361 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     6421 gcaatccatc ttgttcaatc atgcctcgat cgagttgaga gtgaatatga gactctaatt
     6481 ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta
     6541 gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt
     6601 agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca
     6661 gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt
     6721 aattctcatg tatcgataac attaacgttt acaatttcgc gccattcgcc attcaggctg
     6781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     6841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     6901 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt gggtaccggg
     6961 ccccccctcg aggtcgacgg tatcgat
//
