LOCUS       V004353                 7171 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004353
VERSION     V004353
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
   PUBMED   26636338
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 7171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label="nptII"
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             1776..3125
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4720..5379)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5380..6149)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgt taattaaaac ctgcaggatg cctgttctgg
     1801 aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt ttaacgggtg
     1861 atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat attattttgc
     1921 tgattggcga tgggatgggg gactcggaaa ttactgccgc acgtaattat gccgaaggtg
     1981 cgggcggctt ttttaaaggt atagatgcct taccgcttac cgggcaatac actcactatg
     2041 cgctgaataa aaaaaccggc aaaccggact acgtcaccga ctcggctgca tcagcaaccg
     2101 cctggtcaac cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt cacgaaaaag
     2161 atcacccaac gattctggaa atggcaaaag ccgcaggtct ggcgaccggt aacgtttcta
     2221 ccgcagagtt gcaggatgcc acgcccgctg cgctggtggc acatgtgacc tcgcgcaaat
     2281 gctacggtcc gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa aaaggcggaa
     2341 aaggatcgat taccgaacag ctgcttaacg ctcgtgccga cgttacgctt ggcggcggcg
     2401 caaaaacctt tgctgaaacg gcaaccgctg gtgaatggca gggaaaaacg ctgcgtgaac
     2461 aggcacaggc gcgtggttat cagttggtga gcgatgctgc ctcactgaat tcggtgacgg
     2521 aagcgaatca gcaaaaaccc ctgcttggcc tgtttgctga cggcaatatg ccagtgcgct
     2581 ggctaggacc gaaagcaacg taccatggca atatcgataa gcccgcagtc acctgtacgc
     2641 caaatccgca acgtaatgac agtgtaccaa ccctggcgca gatgaccgac aaagccattg
     2701 aattgttgag taaaaatgag aaaggctttt tcctgcaagt tgaaggtgcg tcaatcgata
     2761 aacaggatca tgctgcgaat ccttgtgggc aaattggcga gacggtcgat ctcgatgaag
     2821 ccgtacaacg ggcgctggaa ttcgctaaaa aggagggtaa cacgctggtc atagtcaccg
     2881 ctgatcacgc ccacgccagc cagattgttg cgccggatac caaagctccg ggcctcaccc
     2941 aggcgctaaa taccaaagat ggcgcagtga tggtgatgag ttacgggaac tccgaagagg
     3001 attcacaaga acataccggc agtcagttgc gtattgcggc gtatggcccg catgccgcca
     3061 atgttgttgg actgaccgac cagaccgatc tcttctacac catgaaagcc gctctggggc
     3121 tgaaataaaa ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt tgctgttagc
     3181 aaccagactt aatggcagat cacgggcgca tacgctcatg gttaaaacat gaagagggat
     3241 ggtgctatga aaataacatt actggttacc ttgcttttcg gtctggtttt tttaaccacc
     3301 gtcggcgctg ccgagagaac tttaacccca caacaacagc gtatgacctc ctgtaatcag
     3361 caggcgacgg cgcaggcgtt gaaaggggat gctcgtaaga cctacatgag tgattgcctg
     3421 aagaacagca agtctgcgcc tggcgaaaaa agtttgacgc cacagcagca aaagatgcgc
     3481 gaatgcaata atcaagcaac acaacaatct ctgaaaggtg atgatcgtaa taagtttatg
     3541 agtgcctgcc tcaagaaagc cgcctgatac ctgatagtgc taacgggtga gctacgaaaa
     3601 tggctcaccc gaaatatcat acttctgcct ttagctccgt ctctataatt tgggaaaatt
     3661 gtttctgaat gttcccaaaa ataatgaatg atgaaaactt tttcaaaaaa gcggcggcgc
     3721 acggggagga acctccttta actcctcaaa acgaacatca gcggtccggg ctgcgcttcg
     3781 cccgtcgcgt cagactaccc cgtgcggttg gcctggctgg catgttctta ccgattgctt
     3841 caacgctggt ttcacacccg ccgccgggct ggtggtggct ggtgttggtc ggctgggcgt
     3901 tcgtctggcc gcatttagcc tggcagatag cgagcagggc cgtcgatccg cttagccggg
     3961 aaatttacaa cttaaaaacc gatgcagtat tagcgggaat gtgggtaggc gtaatgggcg
     4021 taaacgtgct gccttccacc gcgatgttga tgattatgtg tctgaatttg atgggggcag
     4081 gcggcccccg tctgtttgtc gcgggtctgg tgttgatggt ggtttcctgc cttgtcaccg
     4141 gccggccgga tccgaattcg cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     4201 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt
     4261 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata
     4321 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg
     4381 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag
     4441 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt
     4501 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc
     4561 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga
     4621 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg
     4681 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg
     4741 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag
     4801 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc
     4861 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg
     4921 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc
     4981 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg
     5041 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt
     5101 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt
     5161 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg
     5221 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat
     5281 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt
     5341 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg
     5401 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa
     5461 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct
     5521 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt
     5581 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc
     5641 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc
     5701 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc
     5761 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc
     5821 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg
     5881 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc
     5941 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg
     6001 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg
     6061 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg
     6121 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg
     6181 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc
     6241 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg
     6301 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag
     6361 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc
     6421 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc
     6481 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga
     6541 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc
     6601 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc
     6661 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc
     6721 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac
     6781 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc
     6841 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca
     6901 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc
     6961 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag
     7021 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg
     7081 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag
     7141 aaggccgatc aagcccaaga gacggcccga g
//
