LOCUS       40924_33028        7165 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB90, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 7165)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label=nptII
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             1769..3106
                     /codon_start=1
                     /product="alkaline phosphatase"
                     /label=alkaline phosphatase
                     /note="derived from Escherichia coli"
                     /protein_id="ALR84553.1"
                     /translation="PVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLI
                     GDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATA
                     WSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRK
                     CYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLR
                     EQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVT
                     CTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVD
                     LDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYG
                     NSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     CDS             complement(4714..5373)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5374..6143)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggt accgagctcg aattcggctt attccctaac taactaaaga
     1741 ttaactttat aaggaggaaa aacatatgcc tgttctggaa aaccgggctg ctcagggcga
     1801 tattactgca cccggcggtg ctcgccgttt aacgggtgat cagactgccg ctctgcgtga
     1861 ttctcttagc gataaacctg caaaaaatat tattttgctg attggcgatg ggatggggga
     1921 ctcggaaatt actgccgcac gtaattatgc cgaaggtgcg ggcggctttt ttaaaggtat
     1981 agatgcctta ccgcttaccg ggcaatacac tcactatgcg ctgaataaaa aaaccggcaa
     2041 accggactac gtcaccgact cggctgcatc agcaaccgcc tggtcaaccg gtgtcaaaac
     2101 ctataacggc gcgctgggcg tcgatattca cgaaaaagat cacccaacga ttctggaaat
     2161 ggcaaaagcc gcaggtctgg cgaccggtaa cgtttctacc gcagagttgc aggatgccac
     2221 gcccgctgcg ctggtggcac atgtgacctc gcgcaaatgc tacggtccga gcgcgaccag
     2281 tgaaaaatgt ccgggtaacg ctctggaaaa aggcggaaaa ggatcgatta ccgaacagct
     2341 gcttaacgct cgtgccgacg ttacgcttgg cggcggcgca aaaacctttg ctgaaacggc
     2401 aaccgctggt gaatggcagg gaaaaacgct gcgtgaacag gcacaggcgc gtggttatca
     2461 gttggtgagc gatgctgcct cactgaattc ggtgacggaa gcgaatcagc aaaaacccct
     2521 gcttggcctg tttgctgacg gcaatatgcc agtgcgctgg ctaggaccga aagcaacgta
     2581 ccatggcaat atcgataagc ccgcagtcac ctgtacgcca aatccgcaac gtaatgacag
     2641 tgtaccaacc ctggcgcaga tgaccgacaa agccattgaa ttgttgagta aaaatgagaa
     2701 aggctttttc ctgcaagttg aaggtgcgtc aatcgataaa caggatcatg ctgcgaatcc
     2761 ttgtgggcaa attggcgaga cggtcgatct cgatgaagcc gtacaacggg cgctggaatt
     2821 cgctaaaaag gagggtaaca cgctggtcat agtcaccgct gatcacgccc acgccagcca
     2881 gattgttgcg ccggatacca aagctccggg cctcacccag gcgctaaata ccaaagatgg
     2941 cgcagtgatg gtgatgagtt acgggaactc cgaagaggat tcacaagaac ataccggcag
     3001 tcagttgcgt attgcggcgt atggcccgca tgccgccaat gttgttggac tgaccgacca
     3061 gaccgatctc ttctacacca tgaaagccgc tctggggctg aaataaaacc gcgcccggca
     3121 gtgaattttc gctgccgggt ggtttttttg ctgttagcaa ccagacttaa tggcagatca
     3181 cgggcgcata cgctcatggt taaaacatga agagggatgg tgctatgaaa ataacattac
     3241 tggttacctt gcttttcggt ctggtttttt taaccaccgt cggcgctgcc gagagaactt
     3301 taaccccaca acaacagcgt atgacctcct gtaatcagca ggcgacggcg caggcgttga
     3361 aaggggatgc tcgtaagacc tacatgagtg attgcctgaa gaacagcaag tctgcgcctg
     3421 gcgaaaaaag tttgacgcca cagcagcaaa agatgcgcga atgcaataat caagcaacac
     3481 aacaatctct gaaaggtgat gatcgtaata agtttatgag tgcctgcctc aagaaagccg
     3541 cctgatacct gatagtgcta acgggtgagc tacgaaaatg gctcacccga aatatcatac
     3601 ttctgccttt agctccgtct ctataatttg ggaaaattgt ttctgaatgt tcccaaaaat
     3661 aatgaatgat gaaaactttt tcaaaaaagc ggcggcgcac ggggaggaac ctcctttaac
     3721 tcctcaaaac gaacatcagc ggtccgggct gcgcttcgcc cgtcgcgtca gactaccccg
     3781 tgcggttggc ctggctggca tgttcttacc gattgcttca acgctggttt cacacccgcc
     3841 gccgggctgg tggtggctgg tgttggtcgg ctgggcgttc gtctggccgc atttagcctg
     3901 gcagatagcg agcagggccg tcgatccgct tagccgggaa atttacaact taaaaaccga
     3961 tgcagtatta gcgggaatgt gggtaggcgt aatgggcgta aacgtgctgc cttccaccgc
     4021 gatgttgatg attatgtgtc tgaatttgat gggggcaggc ggcccccgtc tgtttgtcgc
     4081 gggtctggtg ttgatggtgg tttcctgcct tgtcacctta attaacctgc aggggccggc
     4141 cggatccgaa ttcgcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4201 tcatttttta accaataggc cgactgcgat gagtggcagg gcggggcgta atttttttaa
     4261 ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat aataagcgga
     4321 tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc ggttcagggc agggtcgtta
     4381 aatagccgct tatgtctatt gctggtttac cggtttattg actaccggaa gcagtgtgac
     4441 cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct ctccccgtgg aggtaataat
     4501 tgacgatatg atcatttatt ctgcctccca gagcctgata aaaacggtga atccgttagc
     4561 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     4621 gcggggaggc agacaaggta tagggcggcg aggcggctac agccgatagt ctggaacagc
     4681 gcacttacgg gttgctgcgc aacccaagtg ctaccggcgc ggcagcgtga cccgtgtcgg
     4741 cggctccaac ggctcgccat cgtccagaaa acacggctca tcgggcatcg gcaggcgctg
     4801 ctgcccgcgc cgttcccatt cctccgtttc ggtcaaggct ggcaggtctg gttccatgcc
     4861 cggaatgccg ggctggctgg gcggctcctc gccggggccg gtcggtagtt gctgctcgcc
     4921 cggatacagg gtcgggatgc ggcgcaggtc gccatgcccc aacagcgatt cgtcctggtc
     4981 gtcgtgatca accaccacgg cggcactgaa caccgacagg cgcaactggt cgcggggctg
     5041 gccccacgcc acgcggtcat tgaccacgta ggccgacacg gtgccggggc cgttgagctt
     5101 cacgacggag atccagcgct cggccaccaa gtccttgact gcgtattgga ccgtccgcaa
     5161 agaacgtccg atgagcttgg aaagtgtctt ctggctgacc accacggcgt tctggtggcc
     5221 catctgcgcc acgaggtgat gcagcagcat tgccgccgtg ggtttcctcg caataagccc
     5281 ggcccacgcc tcatgcgctt tgcgttccgt ttgcacccag tgaccgggct tgttcttggc
     5341 ttgaatgccg atttctctgg actgcgtggc catgcttatc tccatgcggt agggtgccgc
     5401 acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca acaattatgc
     5461 gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg agctattgcg
     5521 gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat ttttaagtct
     5581 ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc ctgcggggtt
     5641 cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg
     5701 atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga acccccgcac
     5761 tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg ccttcgactg cccccactcg
     5821 cataggcttg ggtcgttcca ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag
     5881 ccttgacccg ccttccactt ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg
     5941 gaggcttttc cccagagaaa attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt
     6001 tggagccggt gggtatgtgg tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag
     6061 ctcgtgggca ggcgcagcct gtccatcagc ttgtccagca gggttgtcca cgggccgagc
     6121 gaagcgagcc agccggtggc cgctcgcggc catcgtccac atatccacgg gctggcaagg
     6181 gagcgcagcg accgcgcagg gcgaagcccg gagagcaagc ccgtagggcg ccgcagccgc
     6241 cgtaggcggt cacgactttg cgaagcaaag tctagtgagt atactcaagc attgagtggc
     6301 ccgccggagg caccgccttg cgctgccccc gtcgagccgg ttggacacca aaagggaggg
     6361 gcaggcatgg cggcatacgc gatcatgcga tgcaagaagc tggcgaaaat gggcaacgtg
     6421 gcggccagtc tcaagcacgc ctaccgcgag cgcgagacgc ccaacgctga cgccagcagg
     6481 acgccagaga acgagcactg ggcggccagc agcaccgatg aagcgatggg ccgactgcgc
     6541 gagttgctgc cagagaagcg gcgcaaggac gctgtgttgg cggtcgagta cgtcatgacg
     6601 gccagcccgg aatggtggaa gtcggccagc caagaacagc aggcggcgtt cttcgagaag
     6661 gcgcacaagt ggctggcgga caagtacggg gcggatcgca tcgtgacggc cagcatccac
     6721 cgtgacgaaa ccagcccgca catgaccgcg ttcgtggtgc cgctgacgca ggacggcagg
     6781 ctgtcggcca aggagttcat cggcaacaaa gcgcagatga cccgcgacca gaccacgttt
     6841 gcggccgctg tggccgatct agggctgcaa cggggcatcg agggcagcaa ggcacgtcac
     6901 acgcgcattc aggcgttcta cgaggccctg gagcggccac cagtgggcca cgtcaccatc
     6961 agcccgcaag cggtcgagcc acgcgcctat gcaccgcagg gattggccga aaagctggga
     7021 atctcaaagc gcgttgagac gccggaagcc gtggccgacc ggctgacaaa agcggttcgg
     7081 caggggtatg agcctgccct acaggccgcc gcaggagcgc gtgagatgcg caagaaggcc
     7141 gatcaagccc aagagacggc ccgag
//
