LOCUS       40924_32998        5756 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pNB-13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Yang Y, Ma J, Song Z, Wu M.
  TITLE     HIV-1 TAT-mediated protein transduction and subcellular localization
            using novel expression vectors
  JOURNAL   FEBS Lett. 532 (1-2), 36-44 (2002)
  PUBMED    12459459
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Yang YH, Wu M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2002) Department of Molecular and Cellular 
            Biology, School of Life Sciences, University of Science and 
            Technology of China, 96 Jingzai Road, Hefei, Anhui 230026, P.R. 
            China
REFERENCE   3  (bases 1 to 5756)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            532 (1-2), 36-44 (2002)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2002) Department of Molecular and Cellular Biology, School 
            of Life Sciences, University of Science and Technology of China, 96 
            Jingzai Road, Hefei, Anhui 230026, P.R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     terminator      complement(213..260)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    319..383
                     /note="multiple cloning site; MCS"
     CDS             complement(384..401)
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             complement(411..428)
                     /label=6xHis
                     /note="6xHis affinity tag"
     RBS             complement(447..469)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(484..508)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(509..527)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        836..913
                     /label=lacI promoter
     CDS             914..1993
                     /label=lacI
                     /note="lac repressor"
     protein_bind    2009..2030
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             3308..3496
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3601..3743
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3929..4517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4691..5548)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5549..5653)
                     /label=AmpR promoter
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggctttgt tagcagccgg atcctcgagt gcggccgcaa gcttgtcgac ggagctcgaa
      361 ttcggatccg aggtacccat atggctgccg cgcggcacca ggccgctgct gtgatgatga
      421 tgatgatggc tgctgcccat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
      481 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      541 gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
      601 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
      661 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
      721 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      781 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      841 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      901 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      961 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     1021 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     1081 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     1141 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     1201 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     1261 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     1321 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     1381 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     1441 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     1501 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     1561 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     1621 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     1681 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     1741 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     1801 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     1861 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     1921 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1981 ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcaccgg
     2041 gatctcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     2101 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     2161 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     2221 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     2281 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     2341 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     2401 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     2461 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     2521 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     2581 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     2641 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     2701 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     2761 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     2821 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     2881 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     2941 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     3001 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     3061 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     3121 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     3181 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     3241 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     3301 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     3361 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     3421 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     3481 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     3541 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     3601 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     3661 tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg
     3721 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4261 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     4381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4981 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5281 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     5341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5641 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5701 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//
