LOCUS       62056_17740        6060 bp RNA     circular SYN 30-AUG-2020
DEFINITION  Shuttle vector pNATX13-sfaC, complete sequence.
ACCESSION   MT881548
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Chi Z, Chi Z, Kong C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Ocean University of China, Qing Dao, 
            Shandong 266000, China
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2020) Ocean University of China, Qing Dao, Shandong 266000, 
            China"
FEATURES             Location/Qualifiers
     source          1..6060
                     /mol_type="other DNA"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3101..3772
                     /gene="padO"
                     /label=padO
     CDS             3101..3772
                     /codon_start=1
                     /transl_table=11
                     /gene="padO"
                     /product="piperazate synthase"
                     /label=padO
                     /protein_id="QNJ99258.1"
                     /translation="MFVPQHYRTDDRRWPVRIVQDNPLALLMSTRDGRAPFASHVPVIV
                     LPRQREELERTGRWQGAVLHGHMNRANPHWKSLADGQPAGLVFQGPAGYVSPAVYNTSP
                     AVPTWNFTAVHVQGRLKLVADEEATLGVVSATARQLEERFGARWTVEPSVDHFRQILPG
                     VGAFELRVEECDSMFKLSQEKEHEVRHAVMDWCARSPRGRSNDLAAVMRDYYPPTTTWP
                     S"
     protein_bind    complement(3779..3812)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3823..3997)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4041..4607)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(5054..5087)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5666..5682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg gggctaaaaa ccccaacttc ggaaaggggt
     2281 gtatttatta gataaaaaac caacgccttt cggggctcct tggtgattca taataactaa
     2341 acgaatcgca tggccttgcg ccggcgatgg ttcattcaaa tttctgccct atcaactttc
     2401 gatggtagga tagtggccta ccatggtatc aacgggtaac ggggaattag ggttctattc
     2461 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta
     2521 cccaatcccg acacggggag gtagtgacaa taaatactga tacagggctc ttttgggtct
     2581 tgtaattgga atgagtacaa tttaaatccc ttaacgagga acaattggag ggcaagtctg
     2641 gtgccagcag ccgcggtaat tccagctcca atagcgtata ttaaagttgt tgcagttaaa
     2701 aagctcgtag ttgaaccgac agttggctca tcatccgtta catcatcttt ttttctgctg
     2761 gctccgctcg gtgggctccc aacgaagcag caaaaaaagt gagagagaaa aactagcttg
     2821 gcggggcatc cgaagctaga ccctttggct cgcttagtca gtgcgccctc tcacacacac
     2881 tcaaaaaggc cacccctccc gcaccctctt ctcatcaccg tcttcatacc acggttcgtc
     2941 aagcaatcgt atctggtaag ctttgacctc ttgagcgggc tccactttgc tatttcttgg
     3001 atctgctcat tcttttcttc cttcctctct ttctaacctc tcttcagaaa gttcaaccgt
     3061 acttcactca atcttccata catcaccgtc aaacactagt atgttcgtcc cacagcacta
     3121 cagaactgat gatcgccgtt ggcctgtccg tatcgtccaa gacaaccctc tcgctctcct
     3181 tatgtctacc cgcgatggcc gtgctccttt cgcctctcac gtcccagtca tcgtccttcc
     3241 cagacagcgc gaggagctgg agagaactgg ccgctggcaa ggtgctgtcc tccacggtca
     3301 catgaacaga gccaacccac actggaagtc cttggctgat ggtcagcctg ctggtcttgt
     3361 cttccaaggc cctgctggtt acgtctctcc agccgtctac aacacctctc ccgctgtccc
     3421 tacttggaac ttcactgctg tccatgtcca gggccgcctg aagctcgtcg ctgacgagga
     3481 agctactctt ggtgtcgtct ctgctactgc tagacagctg gaggagagat tcggtgctag
     3541 atggactgtc gagccttctg tcgatcactt ccgtcaaatc ctgccaggcg tcggtgcctt
     3601 cgagctcaga gtcgaggagt gcgactctat gttcaagctc tctcaagaga aggagcacga
     3661 agtcagacac gctgtcatgg attggtgcgc ccgctctccc cgtggtagat ctaacgacct
     3721 tgctgccgtc atgcgtgatt actaccctcc aaccactacc tggccttctt aagtcgacta
     3781 ccgttcgtat agcatacatt atacgaagtt attaattcgg gggatctgga ttttagtact
     3841 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
     3901 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     3961 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
     4021 cccggtcggc atctacttta tggacatggc atggacatgt acaaagcctg ttcaccatca
     4081 gaagcagtac cgtcgtacaa agcagtatcc aaaccacaca aggtgaaacc cattcttctg
     4141 taagcgtgga tagctggagc gttgacgttg gtaacctcca accacaagtg accagcacct
     4201 ctctctctag cgaactcagt agccaaaccc atcaaagctc taccgacacc gtgacctctg
     4261 tgttctggag cgacctcgat gtcttcaacg gtcaatcttc tgttccaacc ggagtaagag
     4321 acaacgacga aaccagccaa atcaccatcg tcaccgtaag cgacgaaagt tctggagtct
     4381 ggatcaccgt cctcaccagc gtcggactca tcgtcagatt catcgtctgg gaaaaccttg
     4441 gtcaatggtg gatcgactgg aacttctctc aaagtgaaac cgtcaccagt agcggtgact
     4501 ctgaaaacag tatcagtggt gaaggaaccg tccaaagcct cgatagcttc agcatcacct
     4561 ggaacagaag ttctgtatct gtaagcggta tcgtccaaag tggtcattgt gactgaattg
     4621 gatgtgttag acaggttgat gtgaacgaca gcaacagaga aagacgcaca gagttatata
     4681 caacagagct tgatgggttg ttgtggtgtg tgaggggctt gaatctttcc ccaacagctt
     4741 ccgacgctcg gcatgagggg cagctcaaaa aaaccgcgtc accgacgtgc taccgagatc
     4801 ttcgtgctct ctcgtgtttg cctatcaact gatatcacca atcatgagcc atctctatgt
     4861 acaaactgcc cttggatcac gtgcttcact cgacatctgc accatgtatc cttcttcgaa
     4921 ttgctcatct tgatcacttt attattcaag atgccttgca ccacacgaca tgtctatcga
     4981 tcggcccgta acgccccgtg aagttgccag aacgcctgcc tgtcccgctg tccgatggat
     5041 gcatgaaggt aatataactt cgtatagcat acattatacg aacggtagga tccccaacag
     5101 ggattgccct agtaacggcg agtgaagcgg caacagctca aatttgaaag ctggccttcg
     5161 ggtccgcatt gtaatttgta gaggatgctt tgggtgaaac gccagtctaa gttccttgga
     5221 acaggacgtc atagagggtg agaatcccgt atgtgactgg aaatgttaac ctatgtaaag
     5281 ctccttcgac gagtcgagtt gtttgggaat gcagctctaa atgggaggta aatttcttct
     5341 aaagctaaat attggcgaga gaccgatagc gcacaagtag agtgatcgaa agatgaaaag
     5401 cactttggta agagagttaa aaagcacgtg aaattgttga aagggaagcg cttgcaatca
     5461 gacttgttta aactgttcgg ccggtcttct gaccggttta ctcagtttgg acaggccagc
     5521 atcagtttcg gcggccggat aaaggctttg ggaatgtggc cttcacttcg gtgaaggtgt
     5581 tgtagcccag ggtgtaatac ggccagccgg gactgaggtc cgcgcttcgg ctaggatgct
     5641 ggcgtaatgg ttggaattcc ccgggactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5701 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5761 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5821 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5881 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     5941 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
//
