LOCUS       62056_17735        6066 bp RNA     circular SYN 30-AUG-2020
DEFINITION  Shuttle vector pNATX13-padO, complete sequence.
ACCESSION   MT881547
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Chi Z, Chi Z, Kong C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Ocean University of China, Qing Dao, 
            Shandong 266000, China
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2020) Ocean University of China, Qing Dao, Shandong 266000, 
            China"
FEATURES             Location/Qualifiers
     source          1..6066
                     /mol_type="other DNA"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3101..3778
                     /gene="XF36 RS26795"
                     /label=XF36 RS26795
     CDS             3101..3778
                     /codon_start=1
                     /transl_table=11
                     /gene="XF36 RS26795"
                     /product="piperazate synthase"
                     /label=XF36 RS26795
                     /protein_id="QNJ99255.1"
                     /translation="MVVGMTAETSTVQGALFGDPAVIETTDHGPTATQDPREVARVVGL
                     AQDPGLFVVERTGLVLRADPARPGCADALARHDGDTVAQLLDTGHLRLGGTHHVHHNGD
                     EGPARSVLVPKATRDMVSRWDHLRPIPEPTRAPEPTKAPQRSTGLIGVDVVEPGKALVC
                     LGTTGQGGTVLRDGGRYRVENDHGALVGHASSYRAAARLLARYHGYAPGPVEIEHEHRL
                     HRR"
     protein_bind    complement(3785..3818)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3829..4003)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4047..4613)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(5060..5093)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5672..5688)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg gggctaaaaa ccccaacttc ggaaaggggt
     2281 gtatttatta gataaaaaac caacgccttt cggggctcct tggtgattca taataactaa
     2341 acgaatcgca tggccttgcg ccggcgatgg ttcattcaaa tttctgccct atcaactttc
     2401 gatggtagga tagtggccta ccatggtatc aacgggtaac ggggaattag ggttctattc
     2461 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta
     2521 cccaatcccg acacggggag gtagtgacaa taaatactga tacagggctc ttttgggtct
     2581 tgtaattgga atgagtacaa tttaaatccc ttaacgagga acaattggag ggcaagtctg
     2641 gtgccagcag ccgcggtaat tccagctcca atagcgtata ttaaagttgt tgcagttaaa
     2701 aagctcgtag ttgaaccgac agttggctca tcatccgtta catcatcttt ttttctgctg
     2761 gctccgctcg gtgggctccc aacgaagcag caaaaaaagt gagagagaaa aactagcttg
     2821 gcggggcatc cgaagctaga ccctttggct cgcttagtca gtgcgccctc tcacacacac
     2881 tcaaaaaggc cacccctccc gcaccctctt ctcatcaccg tcttcatacc acggttcgtc
     2941 aagcaatcgt atctggtaag ctttgacctc ttgagcgggc tccactttgc tatttcttgg
     3001 atctgctcat tcttttcttc cttcctctct ttctaacctc tcttcagaaa gttcaaccgt
     3061 acttcactca atcttccata catcaccgtc aaacactagt atggtcgtcg gtatgaccgc
     3121 tgagacctct actgtccagg gcgctctttt cggtgaccct gccgtcatcg agaccactga
     3181 tcacggccct accgccactc aggacccacg tgaggtcgct agagtcgtcg gccttgccca
     3241 agatccaggt ctgttcgtcg tcgagagaac tggtcttgtc ctgcgcgctg atccagctag
     3301 acctggttgc gctgatgctc tggctcgtca cgacggtgat accgtcgctc aactccttga
     3361 tactggccac ctcagacttg gcggtaccca ccatgtccat cacaacggtg atgagggtcc
     3421 agctcgttct gtcctcgtcc ctaaggccac tagagacatg gtctctcgct gggatcacct
     3481 tcgtcctatc ccagagccca ccagagctcc tgagccaact aaggccccac agagatctac
     3541 tggtctcatc ggtgtcgatg tcgtcgagcc tggcaaggct ctggtctgct tgggtaccac
     3601 tggtcaaggc ggtactgtcc tgcgcgatgg cggtagatac cgcgtcgaga acgaccatgg
     3661 tgctttggtc ggtcatgctt cttcttaccg cgctgccgct cgtctgctcg ctagatacca
     3721 cggctacgcc cccggtcctg tcgagatcga gcacgagcac cgtcttcacc gccgttaagt
     3781 cgactaccgt tcgtatagca tacattatac gaagttatta attcggggga tctggatttt
     3841 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
     3901 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
     3961 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg
     4021 acagatcccg gtcggcatct actttatgga catggcatgg acatgtacaa agcctgttca
     4081 ccatcagaag cagtaccgtc gtacaaagca gtatccaaac cacacaaggt gaaacccatt
     4141 cttctgtaag cgtggatagc tggagcgttg acgttggtaa cctccaacca caagtgacca
     4201 gcacctctct ctctagcgaa ctcagtagcc aaacccatca aagctctacc gacaccgtga
     4261 cctctgtgtt ctggagcgac ctcgatgtct tcaacggtca atcttctgtt ccaaccggag
     4321 taagagacaa cgacgaaacc agccaaatca ccatcgtcac cgtaagcgac gaaagttctg
     4381 gagtctggat caccgtcctc accagcgtcg gactcatcgt cagattcatc gtctgggaaa
     4441 accttggtca atggtggatc gactggaact tctctcaaag tgaaaccgtc accagtagcg
     4501 gtgactctga aaacagtatc agtggtgaag gaaccgtcca aagcctcgat agcttcagca
     4561 tcacctggaa cagaagttct gtatctgtaa gcggtatcgt ccaaagtggt cattgtgact
     4621 gaattggatg tgttagacag gttgatgtga acgacagcaa cagagaaaga cgcacagagt
     4681 tatatacaac agagcttgat gggttgttgt ggtgtgtgag gggcttgaat ctttccccaa
     4741 cagcttccga cgctcggcat gaggggcagc tcaaaaaaac cgcgtcaccg acgtgctacc
     4801 gagatcttcg tgctctctcg tgtttgccta tcaactgata tcaccaatca tgagccatct
     4861 ctatgtacaa actgcccttg gatcacgtgc ttcactcgac atctgcacca tgtatccttc
     4921 ttcgaattgc tcatcttgat cactttatta ttcaagatgc cttgcaccac acgacatgtc
     4981 tatcgatcgg cccgtaacgc cccgtgaagt tgccagaacg cctgcctgtc ccgctgtccg
     5041 atggatgcat gaaggtaata taacttcgta tagcatacat tatacgaacg gtaggatccc
     5101 caacagggat tgccctagta acggcgagtg aagcggcaac agctcaaatt tgaaagctgg
     5161 ccttcgggtc cgcattgtaa tttgtagagg atgctttggg tgaaacgcca gtctaagttc
     5221 cttggaacag gacgtcatag agggtgagaa tcccgtatgt gactggaaat gttaacctat
     5281 gtaaagctcc ttcgacgagt cgagttgttt gggaatgcag ctctaaatgg gaggtaaatt
     5341 tcttctaaag ctaaatattg gcgagagacc gatagcgcac aagtagagtg atcgaaagat
     5401 gaaaagcact ttggtaagag agttaaaaag cacgtgaaat tgttgaaagg gaagcgcttg
     5461 caatcagact tgtttaaact gttcggccgg tcttctgacc ggtttactca gtttggacag
     5521 gccagcatca gtttcggcgg ccggataaag gctttgggaa tgtggccttc acttcggtga
     5581 aggtgttgta gcccagggtg taatacggcc agccgggact gaggtccgcg cttcggctag
     5641 gatgctggcg taatggttgg aattccccgg gactggccgt cgttttacaa cgtcgtgact
     5701 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5761 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     5821 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5881 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     5941 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     6001 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     6061 gcgcga
//
