LOCUS       62056_17730        6099 bp RNA     circular SYN 30-AUG-2020
DEFINITION  Shuttle vector pNATX13-matF, complete sequence.
ACCESSION   MT881546
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Chi Z, Chi Z, Kong C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Ocean University of China, Qing Dao, 
            Shandong 266000, China
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2020) Ocean University of China, Qing Dao, Shandong 266000, 
            China"
FEATURES             Location/Qualifiers
     source          1..6099
                     /mol_type="other DNA"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3101..3811
                     /gene="matF"
                     /label=matF
     CDS             3101..3811
                     /codon_start=1
                     /transl_table=11
                     /gene="matF"
                     /product="piperazate synthase"
                     /label=matF
                     /protein_id="QNJ99252.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFVS
                     KRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFYYH
                     GPRDNRERYYRVSFREVPTRNHTRRSPTGGVVSTEPVVVMDTILVVRPRQVQFKWSFDK
                     VTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYNDKFI
                     KISDSCPAKPPSAD"
     protein_bind    complement(3818..3851)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3862..4036)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4080..4646)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(5093..5126)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5705..5721)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg gggctaaaaa ccccaacttc ggaaaggggt
     2281 gtatttatta gataaaaaac caacgccttt cggggctcct tggtgattca taataactaa
     2341 acgaatcgca tggccttgcg ccggcgatgg ttcattcaaa tttctgccct atcaactttc
     2401 gatggtagga tagtggccta ccatggtatc aacgggtaac ggggaattag ggttctattc
     2461 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta
     2521 cccaatcccg acacggggag gtagtgacaa taaatactga tacagggctc ttttgggtct
     2581 tgtaattgga atgagtacaa tttaaatccc ttaacgagga acaattggag ggcaagtctg
     2641 gtgccagcag ccgcggtaat tccagctcca atagcgtata ttaaagttgt tgcagttaaa
     2701 aagctcgtag ttgaaccgac agttggctca tcatccgtta catcatcttt ttttctgctg
     2761 gctccgctcg gtgggctccc aacgaagcag caaaaaaagt gagagagaaa aactagcttg
     2821 gcggggcatc cgaagctaga ccctttggct cgcttagtca gtgcgccctc tcacacacac
     2881 tcaaaaaggc cacccctccc gcaccctctt ctcatcaccg tcttcatacc acggttcgtc
     2941 aagcaatcgt atctggtaag ctttgacctc ttgagcgggc tccactttgc tatttcttgg
     3001 atctgctcat tcttttcttc cttcctctct ttctaacctc tcttcagaaa gttcaaccgt
     3061 acttcactca atcttccata catcaccgtc aaacactagt atgttccgcc gtagaggtgt
     3121 cacccttact aaggctctcc ttaccgccgt ctgcatgctg gctgccccac tcactcaggc
     3181 tatctctgtc ggcaacctta ccttctctct gccctctgag actgacttcg tctctaagcg
     3241 tgtcgtcaac aacaacaagt ctgccagaat ctaccgcatc gctatctctg ccatcgactc
     3301 tccaggttct tctgagctca gaactagacc tgtcgatggt gaactgctct tcgctcctag
     3361 acagctcgct cttcaagccg gcgagtctga gtacttcaag ttctactacc acggccccag
     3421 agataaccgt gagagatact accgtgtctc tttcagagag gtcccaaccc gcaaccacac
     3481 tcgccgttct cccaccggcg gtgtcgtctc tactgagcct gtcgtcgtca tggacaccat
     3541 cctggtcgtc cgccctcgtc aggtccaatt caagtggtct ttcgataagg tcaccggcac
     3601 tgtctctaac accggtaaca cttggttcaa gcttctgatc aagcctggct gcgactctac
     3661 tgaggaagag ggcgatgctt ggtacttgcg cccaggcgat gtcgtccacc agcccgagct
     3721 ccgtcaacct ggtaaccact accttgtcta caacgacaag ttcatcaaga tctctgattc
     3781 ttgcccagct aagcctccat ctgccgatta agtcgactac cgttcgtata gcatacatta
     3841 tacgaagtta ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
     3901 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     3961 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
     4021 cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactttat
     4081 ggacatggca tggacatgta caaagcctgt tcaccatcag aagcagtacc gtcgtacaaa
     4141 gcagtatcca aaccacacaa ggtgaaaccc attcttctgt aagcgtggat agctggagcg
     4201 ttgacgttgg taacctccaa ccacaagtga ccagcacctc tctctctagc gaactcagta
     4261 gccaaaccca tcaaagctct accgacaccg tgacctctgt gttctggagc gacctcgatg
     4321 tcttcaacgg tcaatcttct gttccaaccg gagtaagaga caacgacgaa accagccaaa
     4381 tcaccatcgt caccgtaagc gacgaaagtt ctggagtctg gatcaccgtc ctcaccagcg
     4441 tcggactcat cgtcagattc atcgtctggg aaaaccttgg tcaatggtgg atcgactgga
     4501 acttctctca aagtgaaacc gtcaccagta gcggtgactc tgaaaacagt atcagtggtg
     4561 aaggaaccgt ccaaagcctc gatagcttca gcatcacctg gaacagaagt tctgtatctg
     4621 taagcggtat cgtccaaagt ggtcattgtg actgaattgg atgtgttaga caggttgatg
     4681 tgaacgacag caacagagaa agacgcacag agttatatac aacagagctt gatgggttgt
     4741 tgtggtgtgt gaggggcttg aatctttccc caacagcttc cgacgctcgg catgaggggc
     4801 agctcaaaaa aaccgcgtca ccgacgtgct accgagatct tcgtgctctc tcgtgtttgc
     4861 ctatcaactg atatcaccaa tcatgagcca tctctatgta caaactgccc ttggatcacg
     4921 tgcttcactc gacatctgca ccatgtatcc ttcttcgaat tgctcatctt gatcacttta
     4981 ttattcaaga tgccttgcac cacacgacat gtctatcgat cggcccgtaa cgccccgtga
     5041 agttgccaga acgcctgcct gtcccgctgt ccgatggatg catgaaggta atataacttc
     5101 gtatagcata cattatacga acggtaggat ccccaacagg gattgcccta gtaacggcga
     5161 gtgaagcggc aacagctcaa atttgaaagc tggccttcgg gtccgcattg taatttgtag
     5221 aggatgcttt gggtgaaacg ccagtctaag ttccttggaa caggacgtca tagagggtga
     5281 gaatcccgta tgtgactgga aatgttaacc tatgtaaagc tccttcgacg agtcgagttg
     5341 tttgggaatg cagctctaaa tgggaggtaa atttcttcta aagctaaata ttggcgagag
     5401 accgatagcg cacaagtaga gtgatcgaaa gatgaaaagc actttggtaa gagagttaaa
     5461 aagcacgtga aattgttgaa agggaagcgc ttgcaatcag acttgtttaa actgttcggc
     5521 cggtcttctg accggtttac tcagtttgga caggccagca tcagtttcgg cggccggata
     5581 aaggctttgg gaatgtggcc ttcacttcgg tgaaggtgtt gtagcccagg gtgtaatacg
     5641 gccagccggg actgaggtcc gcgcttcggc taggatgctg gcgtaatggt tggaattccc
     5701 cgggactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5761 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5821 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     5881 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5941 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6001 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6061 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
