LOCUS       62056_17720        6033 bp RNA     circular SYN 30-AUG-2020
DEFINITION  Shuttle vector pNATX13-hmtC, complete sequence.
ACCESSION   MT881545
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Chi Z, Chi Z, Kong C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2020) Ocean University of China, Qing Dao, 
            Shandong 266000, China
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2020) Ocean University of China, Qing Dao, Shandong 266000, 
            China"
FEATURES             Location/Qualifiers
     source          1..6033
                     /mol_type="other DNA"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            3101..3745
                     /gene="hmtC"
                     /label=hmtC
     CDS             3101..3745
                     /codon_start=1
                     /transl_table=11
                     /gene="hmtC"
                     /product="piperazate synthase"
                     /label=hmtC
                     /protein_id="QNJ99249.1"
                     /translation="MFVPSHYREPDSSWMVDIIRGNPLALMMSNGAAGEPPFATHLPVI
                     PDPAMTGDWSERLSEATLLGHMNRDNPQWQALEDGAVVRIAFSGPHAYVSPTLYGVTPA
                     APTWNFTSVHVRGVVERIPSTEETLEVVKSTVRAFEADFGEGWDMAASIDYFRKIVPGV
                     GAFRIMVRNVDGMFKLSQEQQPEVRDRVRKSFAGRECGRHQETAAYMSRLP"
     protein_bind    complement(3752..3785)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(3796..3970)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4014..4580)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     protein_bind    complement(5027..5060)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5639..5655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgccccg gggctaaaaa ccccaacttc ggaaaggggt
     2281 gtatttatta gataaaaaac caacgccttt cggggctcct tggtgattca taataactaa
     2341 acgaatcgca tggccttgcg ccggcgatgg ttcattcaaa tttctgccct atcaactttc
     2401 gatggtagga tagtggccta ccatggtatc aacgggtaac ggggaattag ggttctattc
     2461 cggagaggga gcctgagaaa cggctaccac atccaaggaa ggcagcaggc gcgcaaatta
     2521 cccaatcccg acacggggag gtagtgacaa taaatactga tacagggctc ttttgggtct
     2581 tgtaattgga atgagtacaa tttaaatccc ttaacgagga acaattggag ggcaagtctg
     2641 gtgccagcag ccgcggtaat tccagctcca atagcgtata ttaaagttgt tgcagttaaa
     2701 aagctcgtag ttgaaccgac agttggctca tcatccgtta catcatcttt ttttctgctg
     2761 gctccgctcg gtgggctccc aacgaagcag caaaaaaagt gagagagaaa aactagcttg
     2821 gcggggcatc cgaagctaga ccctttggct cgcttagtca gtgcgccctc tcacacacac
     2881 tcaaaaaggc cacccctccc gcaccctctt ctcatcaccg tcttcatacc acggttcgtc
     2941 aagcaatcgt atctggtaag ctttgacctc ttgagcgggc tccactttgc tatttcttgg
     3001 atctgctcat tcttttcttc cttcctctct ttctaacctc tcttcagaaa gttcaaccgt
     3061 acttcactca atcttccata catcaccgtc aaacactagt atgttcgtcc cttctcacta
     3121 ccgcgagcca gactcttctt ggatggtcga tatcatccgt ggcaacccac tggccctcat
     3181 gatgtctaac ggtgctgctg gcgagcctcc attcgctacc caccttcctg tcatccccga
     3241 ccctgctatg actggcgatt ggtctgagag actgtctgag gccaccctcc ttggtcacat
     3301 gaaccgcgat aaccctcagt ggcaggcttt ggaggatggt gctgtcgtca gaatcgcttt
     3361 ctctggtccc cacgcttacg tctctcctac cctttacggc gtcactccag ctgcccccac
     3421 ctggaacttc acttctgtcc acgtccgtgg tgtcgtcgag agaatccctt ctaccgagga
     3481 gactcttgag gtcgtcaagt ctactgtccg tgctttcgag gccgacttcg gcgagggttg
     3541 ggatatggct gcctctatcg actacttcag aaagatcgtc cctggcgtcg gtgccttccg
     3601 catcatggtc cgtaacgtcg atggcatgtt caagctgtct caggagcagc aaccagaggt
     3661 ccgcgaccgt gtcagaaagt ctttcgctgg cagagagtgc ggtcgccacc aagagaccgc
     3721 tgcctacatg tctcgtctcc cataagtcga ctaccgttcg tatagcatac attatacgaa
     3781 gttattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     3841 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     3901 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
     3961 tatggagaaa ctcgagcttg tcgatcgaca gatcccggtc ggcatctact ttatggacat
     4021 ggcatggaca tgtacaaagc ctgttcacca tcagaagcag taccgtcgta caaagcagta
     4081 tccaaaccac acaaggtgaa acccattctt ctgtaagcgt ggatagctgg agcgttgacg
     4141 ttggtaacct ccaaccacaa gtgaccagca cctctctctc tagcgaactc agtagccaaa
     4201 cccatcaaag ctctaccgac accgtgacct ctgtgttctg gagcgacctc gatgtcttca
     4261 acggtcaatc ttctgttcca accggagtaa gagacaacga cgaaaccagc caaatcacca
     4321 tcgtcaccgt aagcgacgaa agttctggag tctggatcac cgtcctcacc agcgtcggac
     4381 tcatcgtcag attcatcgtc tgggaaaacc ttggtcaatg gtggatcgac tggaacttct
     4441 ctcaaagtga aaccgtcacc agtagcggtg actctgaaaa cagtatcagt ggtgaaggaa
     4501 ccgtccaaag cctcgatagc ttcagcatca cctggaacag aagttctgta tctgtaagcg
     4561 gtatcgtcca aagtggtcat tgtgactgaa ttggatgtgt tagacaggtt gatgtgaacg
     4621 acagcaacag agaaagacgc acagagttat atacaacaga gcttgatggg ttgttgtggt
     4681 gtgtgagggg cttgaatctt tccccaacag cttccgacgc tcggcatgag gggcagctca
     4741 aaaaaaccgc gtcaccgacg tgctaccgag atcttcgtgc tctctcgtgt ttgcctatca
     4801 actgatatca ccaatcatga gccatctcta tgtacaaact gcccttggat cacgtgcttc
     4861 actcgacatc tgcaccatgt atccttcttc gaattgctca tcttgatcac tttattattc
     4921 aagatgcctt gcaccacacg acatgtctat cgatcggccc gtaacgcccc gtgaagttgc
     4981 cagaacgcct gcctgtcccg ctgtccgatg gatgcatgaa ggtaatataa cttcgtatag
     5041 catacattat acgaacggta ggatccccaa cagggattgc cctagtaacg gcgagtgaag
     5101 cggcaacagc tcaaatttga aagctggcct tcgggtccgc attgtaattt gtagaggatg
     5161 ctttgggtga aacgccagtc taagttcctt ggaacaggac gtcatagagg gtgagaatcc
     5221 cgtatgtgac tggaaatgtt aacctatgta aagctccttc gacgagtcga gttgtttggg
     5281 aatgcagctc taaatgggag gtaaatttct tctaaagcta aatattggcg agagaccgat
     5341 agcgcacaag tagagtgatc gaaagatgaa aagcactttg gtaagagagt taaaaagcac
     5401 gtgaaattgt tgaaagggaa gcgcttgcaa tcagacttgt ttaaactgtt cggccggtct
     5461 tctgaccggt ttactcagtt tggacaggcc agcatcagtt tcggcggccg gataaaggct
     5521 ttgggaatgt ggccttcact tcggtgaagg tgttgtagcc cagggtgtaa tacggccagc
     5581 cgggactgag gtccgcgctt cggctaggat gctggcgtaa tggttggaat tccccgggac
     5641 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5701 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5761 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     5821 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     5881 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     5941 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     6001 agaggttttc accgtcatca ccgaaacgcg cga
//
