LOCUS       40924_32978       12721 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pN6-Bxb1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Thomson JG, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2011) Crop Improvement and Utilization Research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   2  (bases 1 to 12721)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2011) Crop Improvement and Utilization Research Unit, 
            USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(320..1342)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1410..2087)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    2278..2299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2314..2344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2352..2368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2376..2392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2402..2458
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2462..2478)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2855..3200
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_recomb     3233..3284
                     /label=Bxb1 recombinase attP attachment site
                     /note="Bxb1 recombinase attP attachment site"
     CDS             3295..4011
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_recomb     4040..4081
                     /label=Bxb1 recombinase attB attachment site
                     /note="Bxb1 recombinase attB attachment site"
     intron          4104..4293
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4288..6111
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVAV
                     KGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             6118..6135
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      6170..6422
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6444..6468
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7768..8394
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8831..9895
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9964..10158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10502..10642
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10828..11416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11506..12297)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
      301 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
      361 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
      421 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
      481 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
      541 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
      601 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
      661 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
      721 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
      781 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
      841 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
      901 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
      961 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
     1021 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     1081 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     1141 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     1201 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     1261 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     1321 cggtgagttc aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga
     1381 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     1441 ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     1501 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     1561 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     1621 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     1681 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     1741 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     1801 tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag
     1861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     1921 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     1981 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     2041 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     2101 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     2161 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2221 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2281 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2341 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2401 cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     2461 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2521 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2581 gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc
     2641 agattgtcgt ttcccgcctt cagtttagct tcatggagtc aaagattcaa atagaggacc
     2701 taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg
     2761 acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata
     2821 tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat
     2881 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
     2941 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
     3001 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     3061 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     3121 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     3181 catttcattt ggagagaaca cgggggactc ttgaccatgg gcgcgccctg cagtggtttg
     3241 tctggtcaac caccgcggtc tcagtggtgt acggtacaaa cccgcggccg caccatggtg
     3301 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3361 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3421 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3481 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3541 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3601 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3661 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3721 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3781 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3841 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3901 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3961 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc
     4021 cctacgctga tcagacgtcc cggcttgtcg acgacggcgg tctccgtcgt caggatcatc
     4081 cgctagtcat ggtagatctg agggtaaatt tctagttttt ctccttcatt ttcttggtta
     4141 ggaccctttt ctctttttat ttttttgagc tttgatcttt ctttaaactg atctattttt
     4201 taattgattg gttatggtgt aaatattaca tagctttaac tgataatctg attactttat
     4261 ttcgtgtgtc tatgatgatg atgatagtta cagaaccgac gactcgtccg tcctgtagaa
     4321 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     4381 aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct
     4441 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac
     4501 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     4561 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     4621 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     4681 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     4741 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     4801 tatgccggaa tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     4861 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     4921 gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     4981 ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     5041 ggttatctct atgaactcga agtcacagcc aaaagccaga cagagtctga tatctacccg
     5101 cttcgcgtcg gcatccggtc agtggcagtg aagggccaac agttcctgat taaccacaaa
     5161 ccgttctact ttactggctt tggtcgtcat gaagatgcgg acttacgtgg caaaggattc
     5221 gataacgtgc tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac
     5281 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     5341 gtggtgattg atgaaactgc tgctgtcggc tttcagctgt ctttaggcat tggtttcgaa
     5401 gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa
     5461 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     5521 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     5581 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     5641 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     5701 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     5761 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     5821 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     5881 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     5941 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     6001 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     6061 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca agctagccac
     6121 caccaccacc accacgtgtg aattacaggt gaccagctcg aatttccccg atcgttcaaa
     6181 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     6241 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     6301 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     6361 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     6421 tcgggaatta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     6481 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     6541 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     6601 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     6661 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     6721 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     6781 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     6841 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     6901 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     6961 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     7021 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     7081 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     7141 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     7201 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     7261 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     7321 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     7381 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     7441 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     7501 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     7561 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     7621 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     7681 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     7741 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     7801 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     7861 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     7921 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     7981 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     8041 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     8101 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     8161 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     8221 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     8281 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     8341 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     8401 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     8461 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     8521 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     8581 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     8641 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     8701 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     8761 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     8821 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     8881 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     8941 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     9001 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     9061 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     9121 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     9181 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     9241 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     9301 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     9361 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     9421 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     9481 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     9541 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     9601 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     9661 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     9721 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     9781 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     9841 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     9901 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     9961 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
    10021 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
    10081 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
    10141 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
    10201 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
    10261 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
    10321 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
    10381 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    10441 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    10501 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    10561 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    10621 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    11221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    11281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    11341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11461 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
    11521 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
    11581 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
    11641 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
    11701 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
    11761 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
    11821 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
    11881 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
    11941 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
    12001 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
    12061 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
    12121 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
    12181 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
    12241 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
    12301 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
    12361 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
    12421 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
    12481 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    12541 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    12601 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    12661 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    12721 g
//
