LOCUS       40924_32953        6969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pN-3xHA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Honda S, Selker EU.
  TITLE     Tools for fungal proteomics: multifunctional neurospora vectors for 
            gene replacement, protein expression and protein purification
  JOURNAL   Genetics 182 (1), 11-23 (2009)
  PUBMED    19171944
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Honda S, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Institute of Molecular Biology, University 
            of Oregon, Eugene, OR 97403, USA
REFERENCE   3  (bases 1 to 6969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2009"; volume: "182"; issue: "1"; pages: "11-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Institute of Molecular Biology, University of Oregon, 
            Eugene, OR 97403, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     4056..4072
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4118..4207
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(4289..4307)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(6968..6969,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggccctaat taaccctcac
     4021 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     4081 gatatcgaat tcctgcagcc caagatctat gatcttttac ccatacgatg ttcctgacta
     4141 tgcgggctat ccgtatgacg tcccggacta tgcaggatcc tatccatatg acgttccaga
     4201 ttacgctggc ggcggaggcg gcggaggcgg cgcgccatta attaactagt tctagagcgg
     4261 ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattaagg gcgtggacgg
     4321 ctaatggggt ctgaatgcta aaagacacca tttcccacac tccctctgat tttttttatg
     4381 gttttttttt atcttcattt ttcgccgtct tttattcggt tgtttgggag gttcacatgc
     4441 aacgtgcaag actgcaagtt ttttgagatg ggcgcttcat gcaatgcagc atcactgcag
     4501 ggacattgtg acatccacgt ttcgatcgtc tgtgcttccc ccgttcatgt acgcatgatc
     4561 tgaaaaaaaa aaaaaaaaaa aaggttgatg taataatgaa aataaacacc cagggacgag
     4621 cacctcatct gctggaatag ggagaaaaac tcaagggccg tttgggtctc cacacttacc
     4681 aacagattca tgtactgatc ttcagcctgt acagaacagt acaatgtcca aaactcgtta
     4741 tcattactct atgtgcccaa aatgacccag atatacatca acccactaca tccttttgtt
     4801 cttgtttttt tttttttttt ttgctacttc gaaaagaatt tccgtttagt tgctcttgct
     4861 catgtgctca agacggatgc tgaccgccct tctgtgagcc tcgagctcct caaccttagc
     4921 caactgcatg acagcctggc cgacgttttt gagaccctcg gcagtcaagt tggagctggt
     4981 aatgtgcttg acgaacgagg cgaaattgac gccagagtac tgcttgccaa agccataggt
     5041 agctatgtgt cttgacgtta gcagaagaat ctgtgaagcg gggctattta tgatatgtaa
     5101 cttacgcagc gagtggttaa caccagcaga gtaatcgcca acggactcag gagtccaggc
     5161 gccaatgaag acactaccgg cgttcatgac aagatcgaca gccttctcgg cctccttgat
     5221 ctggaggatc aagtgctcag gagcgtactt gttgctgagc tccatggcct cctcgacggt
     5281 cttgacctgc acggtgatcg agtgggcgat ggagccacgg acaatctgga cgcgaggaag
     5341 ctccgtagcc tgacggtgaa cctcgtcctc aatagcctga agatgctcct cgtcgaggtc
     5401 aatagcgatc aggatgacct gactgtcaac gccgtgctca gcctgggaca ggagatccga
     5461 ggcaacgaac gcggggttgg cgtccttgtc agcgatgacc agcacctcgg acgggccagc
     5521 gggcatgtca ataccaacgg cagcgttggt gtcgttgctg acgaacatct tggcagcagt
     5581 gacgaactgg ttaccggggc cgagaatctt gtcgaccttg gtgatgctct cggtgccgta
     5641 ggccatggca gctacggcct gggcaccgcc ggcaagcacg atggactcgg ccccaacctt
     5701 gtgagcgacg tggacaatct cgggagtgat ggttccgtcg gcgcggggag gagaggcgaa
     5761 cacaatcttg ttgcagccgg cgaccatggc gggaacaccc agcataaggg cagtgctggg
     5821 gagaacggcg gtaccgccgg ggatgtagca gccgacggcc tcgatgggac gagagaaacg
     5881 gctgcagaca acaccgggca tggtctcgac ctggaggggc ttctcctcct tctgggcggc
     5941 gtggaacttg cggatgttct cgaaggacac gtcgatggca gcaatggtct cctcagggag
     6001 ctgcataagc tccttgggga agggcgcctt caggacgggg ctagtaagag aggtagcctt
     6061 ctcgaacttg tgagtgtacg acagaacagc cttgtcgccg ttcttgcgga cgtcctcgat
     6121 gatggggaca atgatcttgt agatggcatc ggacgacttt tgcgcaggac gcttgagagc
     6181 agcatcgagc tcctcggtag agaccttgga ggcgtcgaaa cgtctcatgg tgatcttctc
     6241 aggggtggtc tcctgggcgg cctccttggt gacaggggcc gaagtggcag cgagagcgga
     6301 cgccgcaggc ttgatgccct ccttctcagc ccacttaccc ttagcatctc cagtcctgcg
     6361 cttgaccttc cagctcttgg cgtcaaggct cctttcgata tcggcaagag taacgccggc
     6421 agcaacggcc ctggtaagag caaagtagaa gagatcggca gcctcaaagg cgatttcctg
     6481 gggggtctga gcggtgcaga gctcctcagc ctcctccatg atcttggccc ggactagctt
     6541 ctcatcggag aagagacggg cagtgtagga gccctcgggg gcagactgtt tcctcgaaat
     6601 caaagtctgc tcgagcttgg gaaggccttt gagctgacca aagcagccgg actgatcgag
     6661 gtggcagaaa cgacccttct gcttcacgac aaacttgaga gcatcgttat cgcagtcaag
     6721 cgagatgcgg acgagctcct gagtgtctcc ggaagtagca cccttgtacc agagaccgcg
     6781 cttccggctc tgatagacac cagtctgtgt cctgagggcc tcgttcacac tctcggcact
     6841 gctgtagacc agacccagag cagtgtcgtg ctcatcaacg acaacggtgg ggagaagacc
     6901 atcgggacgg tcagacttcc agacgctcga gaggatggtg gagatggcaa gcttcagctg
     6961 ctcgagttc
//
