LOCUS       40924_32933        6023 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMyrCAM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Chang C, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Chang C, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6023)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-1999) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Jan 15, 1999 this sequence version replaced gi:4154310.
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..63
                     /codon_start=1
                     /label=myr
                     /note="N-myristoylation signal from Src kinase (Pellman et
                     al., 1985; Kaplan et al., 1988)"
                     /translation="MGSSKSKPKDPSQR"
     terminator      129..376
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(624..1212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1378..2034)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(2482..3282)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3283..3503)
                     /label=URA3 promoter
     rep_origin      complement(4102..4982)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(4993..5506)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5525..5966
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     promoter        5998..6016
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttctag agcttactag tatggggagt agcaagagca agcctaagga ccccagccag
       61 cgccggtcta gagaattcgc ccgggcctcg aggtcgacta attgaataat aagctctaga
      121 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
      181 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
      241 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
      301 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
      361 gaaggcttta atttgcaagc tgcggccctg cattaatgaa tcggccaacg cgcggggaga
      421 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      481 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      541 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaagcc caggaaccgt
      601 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      661 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      721 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      781 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      841 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      901 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      961 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1021 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1081 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1141 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1201 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1261 aactcacgtt aagggatttt ggtcatgagc ttgcgcagac caaaacgatc tcaagaagat
     1321 catcttatta atcagataaa atatttctag gcaccaataa ctgccttaaa aaaattacgc
     1381 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1441 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1501 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1561 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1621 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1681 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1741 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1801 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1861 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1921 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1981 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2041 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2101 attatggtga aagttggaac ctcttaccaa gctcgtcaat acgggataat accgcgccac
     2161 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2221 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2281 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2341 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2401 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     2461 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     2521 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     2581 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     2641 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     2701 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     2761 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     2821 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     2881 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     2941 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     3001 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     3061 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     3121 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     3181 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     3241 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     3301 gcaggttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     3361 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     3421 ttcggagatt accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa
     3481 aaaaaatgat gaattgaatt gaaaagctag cttatcgatg ataagctgtc aaagatgaga
     3541 attaattcca cggactatag actatactag atactccgtc tactgtacga tacacttccg
     3601 ctcaggtcct tgtcctttaa cgaggcctta ccactctttt gttactctat tgatccagct
     3661 cagcaaaggc agtgtgatct aagattctat cttcgcgatg tagtaaaact agctagaccg
     3721 agaaagagac tagaaatgca aaaggcactt ctacaatggc tgccatcatt attatccgat
     3781 gtgacgctgc agcttctcaa tgatattcga atacgctttg aggagataca gcctaatatc
     3841 cgacaaactg ttttacagat ttacgatcgt acttgttacc catcattgaa ttttgaacat
     3901 ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata
     3961 gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct
     4021 attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca
     4081 cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc
     4141 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     4201 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
     4261 aatgcaacgc gacgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag
     4321 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt
     4381 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt
     4441 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt
     4501 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc
     4561 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca
     4621 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag
     4681 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata
     4741 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt
     4801 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg
     4861 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata
     4921 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca
     4981 atgggaagct ccaccccggt tgataatcag aaaagcccca aaaacaggaa gattgtataa
     5041 gcaaatattt aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     5101 atcagctcat tttttaacga atagcccgaa atcggcaaaa tcccttataa atcaaaagaa
     5161 tagaccgaga tagggttgag tgttgttcca gtttccaaca agagtccact attaaagaac
     5221 gtggactcca acgtcaaagg gcgaaaaagg gtctatcagg gcgatggccc actacgtgaa
     5281 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcagtaaa tcggaagggt
     5341 aaacggatgc ccccatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     5401 gggaagaaag cgaaaggagc gggggctagg gcggtgggaa gtgtaggggt cacgctgggc
     5461 gtaaccacca cacccgccgc gcttaatggg gcgctacagg gcgcgtgggg atgatccact
     5521 agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg
     5581 cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc
     5641 cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt
     5701 aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg
     5761 cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc
     5821 tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt
     5881 ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata
     5941 tacctctata ctttaacgtc aaggagaaaa aaccccggat cggactacta gcagctgtaa
     6001 tacgactcac tatagggaat att
//
