LOCUS       40924_31245        5010 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMono_T-vector, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Fursova O, Pogorelko G, Zabotina OA.
  TITLE     An efficient method for transient gene expression in monocots 
            applied to modify the Brachypodium distachyon cell wall
  JOURNAL   Ann. Bot. 110 (1), 47-56 (2012)
  PUBMED    22589326
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Pogorelko GV, Fursova OV, Zabotina OA.
  TITLE     Entry T-vector with monocothyledonous plants specific elements 
            (mono_T-Vector)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5010)
  AUTHORS   Pogorelko GV, Fursova OV, Zabotina OA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2011) BBMB, Iowa State University, ISU, Ames, IA 
            50011, USA
REFERENCE   4  (bases 1 to 5010)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Ann. Bot.";
            date: "2012"; volume: "110"; issue: "1"; pages: "47-56"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (14-SEP-2011) BBMB, Iowa State University, ISU, Ames, IA 50011, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    61..162
                     /label=Zea mays ubiquitin signal peptide sequence
                     /note="Zea mays ubiquitin signal peptide sequence"
     CDS             complement(192..1004)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             1064..1777
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     terminator      1797..2046
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(2135..2153)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2171..2187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2195..2211)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2219..2249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2264..2285)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2573..3161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3335..4192)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4193..4297)
                     /label=AmpR promoter
     rep_origin      complement(4375..4830)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4971..4987
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4994..5010
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 gaattcatgg gatccctcgt taataacatc atggtcgtgg gcgccgtcct tgcggcgctc
      121 gtcgccggcg ggtcgtgcga cgtcgtcgac ccatggggta cccggggatc ccaatactcc
      181 tttggtcatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
      241 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
      301 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
      361 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
      421 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
      481 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
      541 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
      601 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
      661 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
      721 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
      781 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
      841 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
      901 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
      961 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcatatttta aacctcccca
     1021 tacaagtatt ggatcctcta gaggatccga aggagatata acaatgagta aaggagaaga
     1081 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1141 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1201 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
     1261 tggtgttcaa tgcttttcaa gatacccaga tcatatgaag cggcacgact tcttcaagag
     1321 cgccatgcct gagggatacg tgcaggagag gaccatctct ttcaaggacg acgggaacta
     1381 caagacacgt gctgaagtca agtttgaggg agacaccctc gtcaacagga tcgagcttaa
     1441 gggaatcgat ttcaaggagg acggaaacat cctcggccac aagttggaat acaactacaa
     1501 ctcccacaac gtatacatca cggcagacaa acaaaagaat ggaatcaaag ctaacttcaa
     1561 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1621 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1681 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1741 tgctgggatt acacatggca tggatgaact atacaaataa gagctcgaat ttccccgatc
     1801 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     1861 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     1921 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     1981 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     2041 tactaggggc cctcaatcac tagtgaattc gcggccgcct gcaggtcgac catatgggag
     2101 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg
     2161 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     2221 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     2281 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     2341 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2401 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2461 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2521 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2581 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2641 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2701 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2761 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2821 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2881 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2941 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3001 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3061 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3121 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3181 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3241 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3301 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3361 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3421 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3481 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3541 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3601 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3661 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3721 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3781 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3841 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3901 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3961 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4021 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4081 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4141 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4201 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4261 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4321 ctgatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg
     4381 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     4441 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     4501 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4561 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4621 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4681 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4741 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4801 ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg
     4861 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     4921 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     4981 ggccagtgaa ttgtaatacg actcactata
//
