LOCUS       V004687                 7108 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004687
VERSION     V004687
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     An allelic exchange system for compliant genetic manipulation of the
            select agents Burkholderia pseudomallei and Burkholderia mallei
  JOURNAL   Gene 430 (1-2), 123-131 (2009)
   PUBMED   19010402
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Hamad MA, Zajdowicz SL, Holmes RK, Voskuil MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2008) Microbiology, University of Colorado Denver
            School of Medicine, 19th Ave, Aurora, CO 80010, USA
REFERENCE   3  (bases 1 to 7108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 430
            (1-2), 123-131 (2009)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2008) Microbiology, University of Colorado Denver School of
            Medicine, 19th Ave, Aurora, CO 80010, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..992
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    1026..1047
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(2584..2686)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     misc_feature    2836..3855
                     /label="mob region"
                     /note="mob region"
     rep_origin      4079..4848
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4849..5508
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     promoter        5982..6010
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             6042..6962
                     /gene="xylE"
                     /label="Metapyrocatechase"
                     /note="Metapyrocatechase from Pseudomonas putida.
                     Accession#: P06622"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgattaat taaactagtg cttgaattcc
     1021 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
     1081 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     1141 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     1201 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     1261 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     1321 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg
     1381 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
     1441 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
     1501 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
     1561 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     1621 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     1681 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     1741 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     1801 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
     1861 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
     1921 caggcagaca tctgccgcgg aggcctgaat cgccccatca tccagccaga aagtgaggga
     1981 gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt
     2041 tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa
     2101 agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt
     2161 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     2221 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     2281 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     2341 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagc
     2401 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagcta aaaaggccgt
     2461 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     2521 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     2581 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     2641 tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg
     2701 ccaaaagttg gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc
     2761 gaagtgatct tccgtcacag gtatttattc gaagacgaaa gggcctcgtg atacgcctat
     2821 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2881 gaaatgtgcg cgcccgcgtt cctgctggcg ctgggcctgt ttctggcgct ggacttcccg
     2941 ctgttccgtc agcagctttt cgcccacggc cttgatgatc gcggcggcct tggcctgcat
     3001 atcccgattc aacggcccca gggcgtccag aacgggcttc aggcgctccc gaaggtctcg
     3061 ggccgtctct tgggcttgat cggccttctt gcgcatctca cgcgctcctg cggcggcctg
     3121 tagggcaggc tcatacccct gccgaaccgc ttttgtcagc cggtcggcca cggcttccgg
     3181 cgtctcaacg cgctttgaga ttcccagctt ttcggccaat ccctgcggtg cataggcgcg
     3241 tggctcgacc gcttgcgggc tgatggtgac gtggcccact ggtggccgct ccagggcctc
     3301 gtagaacgcc tgaatgcgcg tgtgacgtgc cttgctgccc tcgatgcccc gttgcagccc
     3361 tagatcggcc acagcggccg caaacgtggt ctggtcgcgg gtcatctgcg ctttgttgcc
     3421 gatgaactcc ttggccgaca gcctgccgtc ctgcgtcagc ggcaccacga acgcggtcat
     3481 gtgcgggctg gtttcgtcac ggtggatgct ggccgtcacg atgcgatccg ccccgtactt
     3541 gtccgccagc cacttgtgcg ccttctcgaa gaacgccgcc tgctgttctt ggctggccga
     3601 cttccaccat tccgggctgg ccgtcatgac gtactcgacc gccaacacag cgtccttgcg
     3661 ccgcttctct ggcagcaact cgcgcagtcg gcccatcgct tcatcggtgc tgctggccgc
     3721 ccagtgctcg ttctctggcg tcctgctggc gtcagcgttg ggcgtctcgc gctcgcggta
     3781 ggcgtgcttg agactggccg ccacgttgcc cattttcgcc agcttcttgc atcgcatgat
     3841 cgcgtatgcc gccatgcctg cccctccctt ttggtgtcca accggctcga cgggggcagc
     3901 gcaaggcggt gcctccggcg ggccactcaa tgcttgagta tactcactag actttgcttc
     3961 gcaaagtcgt gaccgcctac ggcggctgcg gcgccctacg ggcttgctct ccgggcttcg
     4021 ccctgcgcgg tcgctgcgct cccttgccag cccgtggata tgtggacgat ggccgcgagc
     4081 ggccaccggc tggctcgctt cgctcggccc gtggacaacc ctgctggaca agctgatgga
     4141 caggctgcgc ctgcccacga gcttgaccac agggattgcc caccggctac ccagccttcg
     4201 accacatacc caccggctcc aactgcgcgg cctgcggcct tgccccatca atttttttaa
     4261 ttttctctgg ggaaaagcct ccggcctgcg gcctgcgcgc ttcgcttgcc ggttggacac
     4321 caagtggaag gcgggtcaag gctcgcgcag cgaccgcgca gcggcttggc cttgacgcgc
     4381 ctggaacgac ccaagcctat gcgagtgggg gcagtcgaag gcgaagcccg cccgcctgcc
     4441 ccccgagcct cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag
     4501 gccgaaggcc gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga
     4561 gccgcgccga aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact
     4621 agatataggg cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca
     4681 acagctcatt gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat
     4741 tgactgccac ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat
     4801 tgcgcatggt gccgcaaccg tgcggcaccc taccgcatgg agataagcat ggccacgcag
     4861 tccagagaaa tcggcattca agccaagaac aagcccggtc actgggtgca aacggaacgc
     4921 aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac ccacggcggc aatgctgctg
     4981 catcacctcg tggcgcagat gggccaccag aacgccgtgg tggtcagcca gaagacactt
     5041 tccaagctca tcggacgttc tttgcggacg gtccaatacg cagtcaagga cttggtggcc
     5101 gagcgctgga tctccgtcgt gaagctcaac ggccccggca ccgtgtcggc ctacgtggtc
     5161 aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc gcctgtcggt gttcagtgcc
     5221 gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt tggggcatgg cgacctgcgc
     5281 cgcatcccga ccctgtatcc gggcgagcag caactaccga ccggccccgg cgaggagccg
     5341 cccagccagc ccggcattcc gggcatggaa ccagacctgc cagccttgac cgaaacggag
     5401 gaatgggaac ggcgcgggca gcagcgcctg ccgatgcccg atgagccgtg ttttctggac
     5461 gatggcgagc cgttggagcc gccgacacgg gtcacgctgc cgcgccggta gcacttgggt
     5521 tgcgcagcaa cccgtaagtg cgctgttcca gactatcggc tgtagccgcc tcgccgccct
     5581 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     5641 aatggaagcc ggcggcacct cgctaacgga ttcaccgttt ttatcaggct ctgggaggca
     5701 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
     5761 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
     5821 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
     5881 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgtag caccagatat
     5941 cagggccccg ctagccagat cttcccggga agcttgagct gttgacaatt aatcatcggc
     6001 tcgtataatg tgtggaatat cacagaagga gacagaatac tatgaacaaa ggtgtaatgc
     6061 gaccgggcca tgtgcagctg cgtgtactgg acatgagcaa ggccctggaa cactacgtcg
     6121 agttgctggg cctgatcgag atggaccgtg acgaccaggg ccgtgtctat ctgaaggctt
     6181 ggaccgaagt ggataagttt tccctggtgc tacgcgaggc tgacgagccg ggcatggatt
     6241 ttatgggttt caaggttgtg gatgaggatg ctctccggca actggagcgg gatctgatgg
     6301 catatggctg tgccgttgag cagctacccg caggtgaact gaacagttgt ggccggcgcg
     6361 tgcgcttcca ggccccctcc gggcatcact tcgagttgta tgcagacaag gaatatactg
     6421 gaaagtgggg tttgaatgac gtcaatcccg aggcatggcc gcgcgatctg aaaggtatgg
     6481 cggctgtgcg tttcgaccac gccctcatgt atggcgacga attgccggcg acctatgacc
     6541 tgttcaccaa ggtgctcggt ttctatctgg ccgaacaggt gctggacgaa aatggcacgc
     6601 gcgtcgccca gtttctcagt ctgtcgacca aggcccacga cgtggccttc attcaccatc
     6661 cggaaaaagg ccgcctccat catgtgtcct tccacctcga aacctgggaa gacttgcttc
     6721 gcgccgccga cctgatctcc atgaccgaca catctatcga tatcggccca acccgccacg
     6781 gcctcactca cggcaagacc atctacttct tcgacccgtc cggtaaccgc aacgaagtgt
     6841 tctgcggggg agattacaac tacccggacc acaaaccggt gacctggacc accgaccagc
     6901 tgggcaaggc gatcttttac cacgaccgca ttctcaacga acgattcatg accgtgctga
     6961 cctgacctga gcggccgccc tgcagcggat ccctctagat gcatgcgacg ggcttgtctg
     7021 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7081 ttttcaccgt catcaccgaa acgcgcga
//
