LOCUS       40924_30690        4250 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for measuring the activity of promoter and enhancer
            sequences using secreted Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..753
                     /codon_start=1
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
                     /translation="MDIKVVFTLVFSALVQAKSTEFDPNIDIVGLEGKFGITNLETDLF
                     TIWETMEVMIKADIADTDRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTR
                     GCLICLSKIKCTAKMKVYIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQF
                     IAQVDRCASCTTGCLKGLANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     polyA_signal    878..999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1006..1461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1488..1592
                     /label=AmpR promoter
     promoter        1594..1951
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1986..2777
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3012..3059
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3388..3976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4038..4086
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4100..4191
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg acatcaaggt ggtgttcacc
      121 ctggtgttca gcgccctggt gcaggccaag agcaccgagt tcgaccccaa catcgacatc
      181 gtgggcctgg aaggcaagtt cggcatcacc aacctggaaa ccgacctgtt caccatctgg
      241 gagaccatgg aagtgatgat caaggccgac atcgccgaca ccgaccgggc cagcaacttc
      301 gtggccaccg agaccgacgc caaccggggc aagatgcccg gcaagaagct gcccctggcc
      361 gtcatcatgg aaatggaagc caacgccttc aaggccggct gcacccgggg ctgcctgatc
      421 tgcctgagca agatcaagtg caccgccaag atgaaggtgt acatccccgg caggtgccac
      481 gactacggcg gcgacaagaa aaccggccag gccggcatcg tgggcgccat cgtggacatc
      541 cccgagatca gcggcttcaa agaaatggcc cccatggaac agttcatcgc ccaggtggac
      601 agatgcgcca gctgcaccac cggctgcctg aagggcctgg ccaacgtgaa gtgcagcgag
      661 ctgctgaaga agtggctgcc cgaccgctgc gccagcttcg ccgacaagat ccagaaagag
      721 gtgcacaaca tcaagggcat ggccggcgac aggtgagcgg ccgcgactct agatcataat
      781 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      841 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      901 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      961 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     1021 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1081 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1141 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1201 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1261 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1321 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1381 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1441 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1561 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     1621 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1681 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     1741 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     1801 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1861 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     1921 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     1981 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     2041 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     2101 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     2161 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     2221 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     2281 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     2341 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     2401 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     2461 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     2521 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2581 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2641 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2701 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2761 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     2821 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     2881 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     2941 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     3001 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     3061 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     3121 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     3181 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     3241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4021 gccttttgct cacatgtaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     4081 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     4141 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tacatgttct
     4201 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
