LOCUS       40924_30685        4781 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for constitutive expression of secreted Metridia 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             628..1284
                     /codon_start=1
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
                     /translation="MDIKVVFTLVFSALVQAKSTEFDPNIDIVGLEGKFGITNLETDLF
                     TIWETMEVMIKADIADTDRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTR
                     GCLICLSKIKCTAKMKVYIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQF
                     IAQVDRCASCTTGCLKGLANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     polyA_signal    1409..1530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1537..1992)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2019..2123
                     /label=AmpR promoter
     promoter        2125..2482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2517..3308
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3543..3590
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3919..4507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4569..4617
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4631..4722
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atccaccggt cgccaccatg gacatcaagg tggtgttcac cctggtgttc
      661 agcgccctgg tgcaggccaa gagcaccgag ttcgacccca acatcgacat cgtgggcctg
      721 gaaggcaagt tcggcatcac caacctggaa accgacctgt tcaccatctg ggagaccatg
      781 gaagtgatga tcaaggccga catcgccgac accgaccggg ccagcaactt cgtggccacc
      841 gagaccgacg ccaaccgggg caagatgccc ggcaagaagc tgcccctggc cgtcatcatg
      901 gaaatggaag ccaacgcctt caaggccggc tgcacccggg gctgcctgat ctgcctgagc
      961 aagatcaagt gcaccgccaa gatgaaggtg tacatccccg gcaggtgcca cgactacggc
     1021 ggcgacaaga aaaccggcca ggccggcatc gtgggcgcca tcgtggacat ccccgagatc
     1081 agcggcttca aagaaatggc ccccatggaa cagttcatcg cccaggtgga cagatgcgcc
     1141 agctgcacca ccggctgcct gaagggcctg gccaacgtga agtgcagcga gctgctgaag
     1201 aagtggctgc ccgaccgctg cgccagcttc gccgacaaga tccagaaaga ggtgcacaac
     1261 atcaagggca tggccggcga caggtgagcg gccgcgactc tagatcataa tcagccatac
     1321 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1381 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1501 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1561 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1621 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1681 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1741 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     1801 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     1861 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     1921 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     1981 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2041 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2101 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2161 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2221 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2281 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2341 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2401 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2461 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2521 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2581 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2641 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     2701 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2761 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2821 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2881 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2941 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3001 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3061 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3121 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3181 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3241 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3301 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3361 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3421 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3481 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3541 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3601 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     3661 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     3721 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     3781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     4081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4561 tcacatgtaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     4621 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     4681 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctacatgttc tttcctgcgt
     4741 tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
