LOCUS       pMetLuc-Mem_Repo        4351 bp DNA     circular SYN 01-JAN-1980
DEFINITION  In vivo imaging vector for measuring promoter activity with 
            membrane-anchored Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pMetLuc-Mem Reporter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..99
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             103..288
                     /codon_start=1
                     /product="signal-anchor region of the transferrin receptor"
                     /label=signal-anchor region of the transferrin receptor
                     /note="TfR membrane anchor"
                     /translation="VDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAV
                     IVFFLIGFMIGYLGYCK"
     CDS             322..927
                     /codon_start=1
                     /product="Metridia luciferase"
                     /label=Metridia luciferase
                     /note="MetLuc"
                     /note="human codon-optimized"
                     /translation="KSTEFDPNIDIVGLEGKFGITNLETDLFTIWETMEVMIKADIADT
                     DRASNFVATETDANRGKMPGKKLPLAVIMEMEANAFKAGCTRGCLICLSKIKCTAKMKV
                     YIPGRCHDYGGDKKTGQAGIVGAIVDIPEISGFKEMAPMEQFIAQVDRCASCTTGCLKG
                     LANVKCSELLKKWLPDRCASFADKIQKEVHNIKGMAGDR"
     polyA_signal    1051..1172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1179..1634)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1661..1765
                     /label=AmpR promoter
     promoter        1834..2044
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             2087..2878
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3113..3160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3489..4077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    4139..4187
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4201..4292
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatga tggtggacgg cgacaacagc
      121 cacgtggaga tgaagctggc cgtggacgag gaagagaacg ccgacaacaa caccaaggcc
      181 aacgtgacca agcccaagcg gtgcagcggc agcatctgct acggcacaat cgccgtgatc
      241 gtgttcttcc tgatcggctt catgatcggc tacctgggct actgcaagtc tagcgacggc
      301 cctggcgaga caggcatgga caagagcacc gagttcgacc ccaacatcga catcgtgggc
      361 ctggaaggca agttcggcat caccaacctg gaaaccgacc tgttcaccat ctgggagacc
      421 atggaagtga tgatcaaggc cgacatcgcc gacaccgacc gggccagcaa cttcgtggcc
      481 accgagaccg acgccaaccg gggcaagatg cccggcaaga agctgcccct ggccgtcatc
      541 atggaaatgg aagccaacgc cttcaaggcc ggctgcaccc ggggctgcct gatctgcctg
      601 agcaagatca agtgcaccgc caagatgaag gtgtacatcc ccggcaggtg ccacgactac
      661 ggcggcgaca agaaaaccgg ccaggccggc atcgtgggcg ccatcgtgga catccccgag
      721 atcagcggct tcaaagaaat ggcccccatg gaacagttca tcgcccaggt ggacagatgc
      781 gccagctgca ccaccggctg cctgaagggc ctggccaacg tgaagtgcag cgagctgctg
      841 aagaagtggc tgcccgaccg ctgcgccagc ttcgccgaca agatccagaa agaggtgcac
      901 aacatcaagg gcatggccgg cgacaggtga gcggcccgac tctagatcat aatcagccat
      961 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     1021 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     1081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     1141 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt
     1201 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     1261 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     1321 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     1381 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     1441 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     1501 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     1561 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     1621 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1681 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1741 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     1801 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     1861 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2041 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     2101 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     2161 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     2221 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     2281 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     2341 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     2401 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     2461 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     2521 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     2581 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     2641 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     2701 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     2761 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     2821 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     2881 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     2941 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     3001 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     3061 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     3121 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     3181 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     3241 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     3301 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     3361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtaa
     4141 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     4201 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     4261 cagtgcaagt gcaggtgcca gaacatttct ctacatgttc tttcctgcgt tatcccctga
     4321 ttctgtggat aaccgtatta ccgccatgca t
//
