LOCUS       pMelBac_C.        4829 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expression and secretion of 
            recombinant proteins. For other reading frames, use pMelBac A or 
            pMelBac B.
ACCESSION   .
VERSION     .
KEYWORDS    pMelBac C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Exclusively for use with Bac-N-Blue(TM) DNA.
FEATURES             Location/Qualifiers
     source          1..4829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     96..158
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    166..221
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     233..938
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1514..1618
                     /label=AmpR promoter
     CDS             1619..2476
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2650..3238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3415..4517)
                     /codon_start=3
                     /gene="lacZ (truncated)"
                     /product="N-terminal fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="5' lacZ fragment"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYTDRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQD"
     CDS             complement(4525..4614)
                     /codon_start=1
                     /gene="Autographa californica Ac-pcna"
                     /product="proliferating cell nuclear antigen (PCNA) from 
                     baculovirus"
                     /label=Autographa californica Ac-pcna
                     /note="Ac PCNA"
                     /translation="MHARLNKSSHRRRKISCIKEQLQLESLLKM"
     promoter        complement(4615..4829)
                     /note="ETL promoter"
                     /note="baculovirus early-to-late promoter (Liu et al.,
                     2006)"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatatgaa attcttagtc aacgttgccc
      121 ttgtttttat ggtcgtatac atttcttaca tctatgcgga tcgatggatc cgacctcgag
      181 atctgcagct ggtaccatgg aattcgaagc ttggagtcga ctctgctgaa gaggaggaaa
      241 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt
      301 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag
      361 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
      421 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
      481 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
      541 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
      601 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
      661 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
      721 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca tatttaacat
      781 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
      841 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
      901 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt
      961 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg
     1021 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg
     1081 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag
     1141 gtggtggcgg tgatgcagac ggcggtttag gctcaaattg tctctttcag gcaacacagt
     1201 cggcacctca actattgtac tggtttcggg cgtatggtgc actctcagta caatctgctc
     1261 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1321 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1381 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1621 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1681 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1741 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1801 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1861 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1921 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1981 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2041 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2101 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2161 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2221 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2281 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2341 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2401 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2461 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2521 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2581 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2641 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2701 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2761 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2821 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2881 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2941 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3001 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3061 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3121 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3181 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3241 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3301 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3361 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcatcctg
     3421 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac
     3481 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc
     3541 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc
     3601 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt
     3661 cagacgtagt gtgacgcgat cggtataacc accacgctca tcgataattt caccgccgaa
     3721 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag
     3781 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc
     3841 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa
     3901 cgagacgtca cggaaaatgc cgctcatccg ccacatatcc tgatcttcca gataactgcc
     3961 gtcactccaa cgcagcacca tcaccgcgag gcggttttct ccggcgcgta aaaatgcgct
     4021 caggtcaaat tcagacggca aacgactgtc ctggccgtaa ccgacccagc gcccgttgca
     4081 ccacagatga aacgccgagt taacgccatc aaaaataatt cgcgtctggc cttcctgtag
     4141 ccagctttca tcaacattaa atgtgagcga gtaacaaccc gtcggattct ccgtgggaac
     4201 aaacggcgga ttgaccgtaa tgggataggt cacgttggtg tagatgggcg catcgtaacc
     4261 gtgcatctgc cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag
     4321 ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag
     4381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     4441 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     4501 acgttgtaaa acgacgggat ctatcatttt tagcagtgat tctaattgca gctgctcttt
     4561 gatacaacta attttacgac gacgatgcga gcttttattc aaccgagcgt gcatgtttgc
     4621 aatcgtgcaa gcgttatcaa tttttcatta tcgtattgtt gcacatcaac aggctggaca
     4681 ccacgttgaa ctcgccgcag ttttgcggca agttggaccc gccgcgcatc caatgcaaac
     4741 tttccgacat tctgttgcct acgaacgatt gattctttgt ccattgatcg aagcgagtgc
     4801 cttcgacttt ttcgtgtcca gtgtggctt
//
