LOCUS       pMelBac_A.        4831 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector for expression and secretion of 
            recombinant proteins. For other reading frames, use pMelBac B or 
            pMelBac C.
ACCESSION   .
VERSION     .
KEYWORDS    pMelBac A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Exclusively for use with Bac-N-Blue(TM) DNA.
FEATURES             Location/Qualifiers
     source          1..4831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..95
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     96..158
                     /label=melittin signal sequence
                     /note="signal sequence from honeybee melittin"
     misc_feature    168..223
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     235..940
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     promoter        1516..1620
                     /label=AmpR promoter
     CDS             1621..2478
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2652..3240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3417..4519)
                     /codon_start=3
                     /gene="lacZ (truncated)"
                     /product="N-terminal fragment of beta-galactosidase"
                     /label=lacZ (truncated)
                     /note="5' lacZ fragment"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYTDRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQD"
     CDS             complement(4527..4616)
                     /codon_start=1
                     /gene="Autographa californica Ac-pcna"
                     /product="proliferating cell nuclear antigen (PCNA) from 
                     baculovirus"
                     /label=Autographa californica Ac-pcna
                     /note="Ac PCNA"
                     /translation="MHARLNKSSHRRRKISCIKEQLQLESLLKM"
     promoter        complement(4617..4831)
                     /note="ETL promoter"
                     /note="baculovirus early-to-late promoter (Liu et al.,
                     2006)"
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatatgaa attcttagtc aacgttgccc
      121 ttgtttttat ggtcgtatac atttcttaca tctatgcgga tcgatgggga tccgagctcg
      181 agatctgcag ctggtaccat ggaattcgaa gcttggagtc gactctgctg aagaggagga
      241 aattctcctt gaagtttccc tggtgttcaa agtaaaggag tttgcaccag acgcacctct
      301 gttcactggt ccggcgtatt aaaacacgat acattgttat tagtacattt attaagcgct
      361 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
      421 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
      481 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
      541 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
      601 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
      661 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
      721 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aaagcctgga catatttaac
      781 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
      841 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
      901 cacgtccgcg atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt
      961 ttgggcgggt ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga
     1021 tggtgcaggc ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc
     1081 tgatgataaa tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg
     1141 aggtggtggc ggtgatgcag acggcggttt aggctcaaat tgtctctttc aggcaacaca
     1201 gtcggcacct caactattgt actggtttcg ggcgtatggt gcactctcag tacaatctgc
     1261 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1321 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1381 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1441 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1861 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2161 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2761 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcatcc
     3421 tgcaccatcg tctgctcatc catgacctga ccatgcagag gatgatgctc gtgacggtta
     3481 acgcctcgaa tcagcaacgg cttgccgttc agcagcagca gaccattttc aatccgcacc
     3541 tcgcggaaac cgacatcgca ggcttctgct tcaatcagcg tgccgtcggc ggtgtgcagt
     3601 tcaaccaccg cacgatagag attcgggatt tcggcgctcc acagtttcgg gttttcgacg
     3661 ttcagacgta gtgtgacgcg atcggtataa ccaccacgct catcgataat ttcaccgccg
     3721 aaaggcgcgg tgccgctggc gacctgcgtt tcaccctgcc ataaagaaac tgttacccgt
     3781 aggtagtcac gcaactcgcc gcacatctga acttcagcct ccagtacagc gcggctgaaa
     3841 tcatcattaa agcgagtggc aacatggaaa tcgctgattt gtgtagtcgg tttatgcagc
     3901 aacgagacgt cacggaaaat gccgctcatc cgccacatat cctgatcttc cagataactg
     3961 ccgtcactcc aacgcagcac catcaccgcg aggcggtttt ctccggcgcg taaaaatgcg
     4021 ctcaggtcaa attcagacgg caaacgactg tcctggccgt aaccgaccca gcgcccgttg
     4081 caccacagat gaaacgccga gttaacgcca tcaaaaataa ttcgcgtctg gccttcctgt
     4141 agccagcttt catcaacatt aaatgtgagc gagtaacaac ccgtcggatt ctccgtggga
     4201 acaaacggcg gattgaccgt aatgggatag gtcacgttgg tgtagatggg cgcatcgtaa
     4261 ccgtgcatct gccagtttga ggggacgacg acagtatcgg cctcaggaag atcgcactcc
     4321 agccagcttt ccggcaccgc ttctggtgcc ggaaaccagg caaagcgcca ttcgccattc
     4381 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     4441 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     4501 cgacgttgta aaacgacggg atctatcatt tttagcagtg attctaattg cagctgctct
     4561 ttgatacaac taattttacg acgacgatgc gagcttttat tcaaccgagc gtgcatgttt
     4621 gcaatcgtgc aagcgttatc aatttttcat tatcgtattg ttgcacatca acaggctgga
     4681 caccacgttg aactcgccgc agttttgcgg caagttggac ccgccgcgca tccaatgcaa
     4741 actttccgac attctgttgc ctacgaacga ttgattcttt gtccattgat cgaagcgagt
     4801 gccttcgact ttttcgtgtc cagtgtggct t
//
