LOCUS       V013013                 7934 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013013
VERSION     V013013
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    110..231
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(500..1088)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1262..2119)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2120..2224)
                     /label="AmpR promoter"
     terminator      2393..2420
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        2505..2834
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2873..3991
                     /gene="GLUL"
                     /label="Glutamine synthetase"
                     /note="Glutamine synthetase from Acomys cahirinus.
                     Accession#: Q9QY94"
     intron          4126..4191
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4321..4341
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4766..4900
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        5471..5850
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5851..6054
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          6190..7016
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             7182..7499
                     /label="hIg-kappa-CL"
                     /note="Human immunoglobulin kappa light chain constant
                     region"
     polyA_signal    7712..7760
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 attgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
       61 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      241 gcggccgcga cctgcaggcg cagaactggt aggtatggaa gatccctcga gatccattgt
      301 gctggcggta ggcgagcagc gcctgcctga agctgcgggc attcccagtc agaaatgagc
      361 gccagtcgtc gtcggctctc ggcaccgaag tgctatgatt ctccgccagc atggcttcgg
      421 ccagtgcgtc gagcagcgcc cgcttgttcc tgaagtgcca gtaaagcgcc ggtcgacctc
      481 gggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      541 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      601 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      661 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      721 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      781 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      841 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      901 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      961 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1021 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1081 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1141 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1201 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1261 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1321 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1381 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1441 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1501 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1561 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1621 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1681 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1741 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1801 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1861 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1921 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1981 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     2041 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2101 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2161 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2221 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     2281 taacctataa aaataggcgt atcacgaggc cctgatggct ctttgcggca cccatcgttc
     2341 gtaatgttcc gtggcaccga ggacaaccct caagagaaaa tgtaatcaca ctggctcacc
     2401 ttcgggtggg cctttctgcg tttataagga gacactttat gtttaagaag gttggtaaat
     2461 tccttgcggc tttggcagcc aagctagaga tccggctgtg gaatgtgtgt cagttagggt
     2521 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2581 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2641 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2701 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2761 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2821 taggcttttg caaaaagcta gcttggggcc accgctcaga gcaccttcca ccatggccac
     2881 ctcagcaagt tcccacttga acaaaaacat caagcaaatg tacttgtgcc tgccccaggg
     2941 tgagaaagtc caagccatgt atatctgggt tgatggtact ggagaaggac tgcgctgcaa
     3001 aacccgcacc ctggactgtg agcccaagtg tgtagaagag ttacctgagt ggaattttga
     3061 tggctctagt acctttcagt ctgagggctc caacagtgac atgtatctca gccctgttgc
     3121 catgtttcgg gaccccttcc gcagagatcc caacaagctg gtgttctgtg aagttttcaa
     3181 gtacaaccgg aagcctgcag agaccaattt aaggcactcg tgtaaacgga taatggacat
     3241 ggtgagcaac cagcacccct ggtttggaat ggaacaggag tatactctga tgggaacaga
     3301 tgggcaccct tttggttggc cttccaatgg ctttcctggg ccccaaggtc cgtattactg
     3361 tggtgtgggc gcagacaaag cctatggcag ggatatcgtg gaggctcact accgcgcctg
     3421 cttgtatgct ggggtcaaga ttacaggaac aaatgctgag gtcatgcctg cccagtggga
     3481 gttccaaata ggaccctgtg aaggaatccg catgggagat catctctggg tggcccgttt
     3541 catcttgcat cgagtatgtg aagactttgg ggtaatagca acctttgacc ccaagcccat
     3601 tcctgggaac tggaatggtg caggctgcca taccaacttt agcaccaagg ccatgcggga
     3661 ggagaatggt ctgaagcaca tcgaggaggc catcgagaaa ctaagcaagc ggcaccggta
     3721 ccacattcga gcctacgatc ccaagggggg cctggacaat gcccgtcgtc tgactgggtt
     3781 ccacgaaacg tccaacatca acgacttttc tgctggtgtc gccaatcgca gtgccagcat
     3841 ccgcattccc cggactgtcg gccaggagaa gaaaggttac tttgaagacc gccgcccctc
     3901 tgccaattgt gacccctttg cagtgacaga agccatcgtc cgcacatgcc ttctcaatga
     3961 gactggcgac gagcccttcc aatacaaaaa ctaattagac tttgagtgat cttgagcctt
     4021 tcctagttca tcccaccccg ccccagagag atctttgtga aggaacctta cttctgtggt
     4081 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     4141 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     4201 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     4261 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     4321 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     4381 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     4441 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     4501 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     4561 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     4621 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     4681 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4741 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4801 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4861 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tctagcttcg tgtcaaggac
     4921 ggtgactgca gtgaataata aaatgtgtgt ttgtccgaaa tacgcgtttt gagatttctg
     4981 tcgccgacta aattcatgtc gcgcgatagt ggtgtttatc gccgatagag atggcgatat
     5041 tggaaaaatc gatatttgaa aatatggcat attgaaaatg tcgccgatgt gagtttctgt
     5101 gtaactgata tcgccatttt tccaaaagtg atttttgggc atacgcgata tctggcgata
     5161 gcgcttatat cgtttacggg ggatggcgat agacgacttt ggtgacttgg gcgattctgt
     5221 gtgtcgcaaa tatcgcagtt tcgatatagg tgacagacga tatgaggcta tatcgccgat
     5281 agaggcgaca tcaagctggc acatggccaa tgcatatcga tctatacatt gaatcaatat
     5341 tggccattag ccatattatt cattggttat atagcataaa tcaatattgg ctattggcca
     5401 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
     5461 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     5521 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5581 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5641 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5701 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     5761 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     5821 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     5881 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5941 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     6001 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     6061 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     6121 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
     6181 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcttatgca
     6241 tgctatactg tttttggctt ggggtctata cacccccgct tcctcatgtt ataggtgatg
     6301 gtatagctta gcctataggt gtgggttatt gaccattatt gaccactccc ctattggtga
     6361 cgatactttc cattactaat ccataacatg gctctttgcc acaactctct ttattggcta
     6421 tatgccaata cactgtcctt cagagactga cacggactct gtatttttac aggatggggt
     6481 ctcatttatt atttacaaat tcacatatac aacaccaccg tccccagtgc ccgcagtttt
     6541 tattaaacat aacgtgggat ctccacgcga atctcgggta cgtgttccgg acatgggctc
     6601 ttctccggta gcggcggagc ttctacatcc gagccctgct cccatgcctc cagcgactca
     6661 tggtcgctcg gcagctcctt gctcctaaca gtggaggcca gacttaggca cagcacgatg
     6721 cccaccacca ccagtgtgcc gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag
     6781 ctcggggagc gggcttgcac cgctgacgca tttggaagac ttaaggcagc ggcagaagaa
     6841 gatgcaggca gctgagttgt tgtgttctga taagagtcag aggtaactcc cgttgcggtg
     6901 ctgttaacgg tggagggcag tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc
     6961 agacataata gctgacagac taacagactg ttcctttcca tgggtctttt ctgcagtcac
     7021 cgtccttgac acgaagcttc gtgagttcta gaggatccat ctgggataag catgctgttt
     7081 tctgtctgtc cctaacatgc cctgtgatta tccgcaaaca acacacccaa gggcagaact
     7141 ttgttactta aacaccatcc tgtttgcttc tttcctcagg aactgtggct gcaccatctg
     7201 tcttcatctt cccgccatct gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc
     7261 tgctgaataa cttctatccc agagaggcca aagtacagtg gaaggtggat aacgccctcc
     7321 aatcgggtaa ctcccaggag agtgtcacag agcaggacag caaggacagc acctacagcc
     7381 tcagcagcac cctgacgctg agcaaagcag actacgagaa acacaaagtc tacgcctgcg
     7441 aagtcaccca tcagggcctg agctcgcccg tcacaaagag cttcaacagg ggagagtgtt
     7501 agagggagaa gtgcccccac ctgctcctca gttccagcct gaccccctcc catcctttgg
     7561 cctctgaccc tttttccaca ggggacctac ccctattgcg gtcctccagc tcatctttca
     7621 cctcaccccc ctcctcctcc ttggctttaa ttatgctaat gttggaggag aatgaataaa
     7681 taaagtgaat ctttgcacct gtggtggatc taataaaaga tatttatttt cattagatat
     7741 gtgtgttggt tttttgtgtg cagtgcctct atctggaggc caggtagggc tggccttggg
     7801 ggagggggag gccagaatga ctccaagagc tacaggaagg caggtcagag accccactgg
     7861 acaaacagtg gctggactct gcaccataac acacaatcaa caggggagtg agctggaaat
     7921 ttgctagcga attc
//
