LOCUS       62056_16460        6603 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        1098..1477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1478..1681
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          1817..2643
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             2778..3767
                     /codon_start=1
                     /label=hlgG1-CH
                     /note="Human IgG1 heavy chain constant region"
                     /translation="ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA
                     LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCD
                     KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD
                     GVEVHNAKTKPREEQYNATYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIAATISKA
                     KGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL
                     DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK"
     polyA_signal    3886..3934
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        complement(4415..4433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4454..4470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4478..4494)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4502..4532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4547..4568)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4856..5444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5618..6475)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6476..6580)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgccgat atttgaaaat atggcatatt gaaaatgtcg ccgatgtgag
      721 tttctgtgta actgatatcg ccatttttcc aaaagtgatt tttgggcata cgcgatatct
      781 ggcgatagcg cttatatcgt ttacggggga tggcgataga cgactttggt gacttgggcg
      841 attctgtgtg tcgcaaatat cgcagtttcg atataggtga cagacgatat gaggctatat
      901 cgccgataga ggcgacatca agctggcaca tggccaatgc atatcgatct atacattgaa
      961 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
     1021 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     1081 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     1141 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1201 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1261 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1321 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1381 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1441 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1501 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1561 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1621 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     1681 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     1741 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
     1801 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
     1861 ttatgcatgc tatactgttt ttggcttggg gtctatacac ccccgcttcc tcatgttata
     1921 ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac cactccccta
     1981 ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca actctcttta
     2041 ttggctatat gccaatacac tgtccttcag agactgacac ggactctgta tttttacagg
     2101 atggggtctc atttattatt tacaaattca catatacaac accaccgtcc ccagtgcccg
     2161 cagtttttat taaacataac gtgggatctc cacgcgaatc tcgggtacgt gttccggaca
     2221 tgggctcttc tccggtagcg gcggagcttc tacatccgag ccctgctccc atgcctccag
     2281 cgactcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac ttaggcacag
     2341 cacgatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt atgtgtctga
     2401 aaatgagctc ggggagcggg cttgcaccgc tgacgcattt ggaagactta aggcagcggc
     2461 agaagaagat gcaggcagct gagttgttgt gttctgataa gagtcagagg taactcccgt
     2521 tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg ctgccgcgcg
     2581 cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg gtcttttctg
     2641 cagtcaccgt ccttgacacg aagcttctga cctcggagga cagcgccgtc tactactgcg
     2701 cccggtcgac gtactacggc ggcgactggt acttcaacgt ctggggggcc ggcacgaccg
     2761 tgaccgtgag cgcggcggct agcaccaagg gcccatcggt cttccccctg gcaccctcct
     2821 ccaagagcac ctctgggggc acagcggccc tgggctgcct ggtcaaggac tacttccccg
     2881 aaccggtgac ggtgtcgtgg aactcaggcg ccctgaccag cggcgtgcac accttcccgg
     2941 ctgtcctaca gtcctcagga ctctactccc tcagcagcgt ggtgaccgtg ccctccagca
     3001 gcttgggcac ccagacctac atctgcaacg tgaatcacaa gcccagcaac accaaggtgg
     3061 acaagagagt tgagcccaaa tcttgtgaca aaactcacac atgcccaccg tgcccagcac
     3121 ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca
     3181 tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg
     3241 aggtcaagtt caactggtat gtggacggcg tggaggtgca taatgccaag acaaagccgc
     3301 gggaggagca gtacaacgcc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccaag
     3361 actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca
     3421 tcgccgcaac catctccaaa gccaaagggc agccccgaga accacaggtg tacaccctgc
     3481 ccccatcccg ggaggagatg accaagaacc aagtcagcct gacctgcctg gtcaaaggct
     3541 tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca
     3601 agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctattcc aagctcaccg
     3661 tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg catgaggctc
     3721 tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggcaaatga gtgcgacggc
     3781 cggcaagccc cgctccccgg gctctcgcgg tcgcacgagg atgcttggca cgtacccgtc
     3841 tacatacttc ccaggcaccc agcatggaaa taaagcaccg gatctaataa aagatattta
     3901 ttttcattag atatgtgtgt tggttttttg tgtgcagtgc ctctatctgg aggccaggta
     3961 gggctggcct tgggggaggg ggaggccaga atgactccaa gagctacagg aaggcaggtc
     4021 agagacccca ctggacaaac agtggctgga ctctgcacca taacacacaa tcaacagggg
     4081 agtgagctgg aaatttgcta gatcctctac gccggacgca tcgtggccgg catcaccggc
     4141 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     4201 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     4261 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     4321 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     4381 cctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     4441 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4501 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4561 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4621 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4681 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4741 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4801 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4861 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4981 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5041 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5101 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5161 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5221 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5281 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5341 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5401 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5461 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5521 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5581 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5641 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5701 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5761 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5821 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5881 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5941 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6001 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6061 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6121 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6181 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6241 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6301 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6361 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6421 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6481 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6541 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6601 cac
//
