LOCUS       V013012                10010 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013012
VERSION     V013012
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          142..968
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     promoter        complement(1774..1792)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1818..2042
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2088..2516
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2521..2864
                     /label="SV40"
                     /note="Simian virus 40 enhancer/early promoter"
     CDS             2926..3717
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3894..4015
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(4112..4142)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4157..4178)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4466..5054)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5228..6085)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6086..6190)
                     /label="AmpR promoter"
     terminator      6359..6386
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        6469..6798
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6837..7955
                     /gene="GLUL"
                     /label="Glutamine synthetase"
                     /note="Glutamine synthetase from Acomys cahirinus.
                     Accession#: Q9QY94"
     intron          8090..8155
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8285..8305
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8730..8864
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        9433..9812
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat tggaacgcgg
      121 attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc cacccccttg
      181 gcttcttatg catgctatac tgtttttggc ttggggtcta tacacccccg cttcctcatg
      241 ttataggtga tggtatagct tagcctatag gtgtgggtta ttgaccatta ttgaccactc
      301 ccctattggt gacgatactt tccattacta atccataaca tggctctttg ccacaactct
      361 ctttattggc tatatgccaa tacactgtcc ttcagagact gacacggact ctgtattttt
      421 acaggatggg gtctcattta ttatttacaa attcacatat acaacaccac cgtccccagt
      481 gcccgcagtt tttattaaac ataacgtggg atctccacgc gaatctcggg tacgtgttcc
      541 ggacatgggc tcttctccgg tagcggcgga gcttctacat ccgagccctg ctcccatgcc
      601 tccagcgact catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg
      661 cacagcacga tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg
      721 tctgaaaatg agctcgggga gcgggcttgc accgctgacg catttggaag acttaaggca
      781 gcggcagaag aagatgcagg cagctgagtt gttgtgttct gataagagtc agaggtaact
      841 cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc
      901 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt
      961 ttctgcagtc accgtccttg acacgaagct tggtaccgag ctcggatcca ctagtaacgg
     1021 ccgccagtgt gctggaattc gagcccaaat cttgtgacaa aactcacaca tgcccaccgt
     1081 gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg
     1141 acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg
     1201 aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga
     1261 caaagccgcg ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc
     1321 tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc
     1381 cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt
     1441 acaccctgcc cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg
     1501 tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga
     1561 acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca
     1621 agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc
     1681 atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg ggtaaatgag
     1741 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     1801 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1861 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1921 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     1981 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2041 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     2101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2161 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2281 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2401 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2461 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     2521 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2581 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2761 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     2821 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     2881 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2941 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3001 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3061 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3121 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3181 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3241 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3301 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3361 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3421 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     3481 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3541 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3601 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3661 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3721 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3781 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3841 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     3901 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3961 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4021 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     4081 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4141 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4201 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4261 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5521 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5821 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6061 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6121 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6181 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6241 tgacattaac ctataaaaat aggcgtatca cgaggccctg atggctcttt gcggcaccca
     6301 tcgttcgtaa tgttccgtgg caccgaggac aaccctcaag agaaaatgta atcacactgg
     6361 ctcaccttcg ggtgggcctt tctgcgttta taaggagaca ctttatgttt aagaaggttg
     6421 gtaaattcct tgcggctttg gcagccaagc tagatccggc tgtggaatgt gtgtcagtta
     6481 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6541 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6601 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6661 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6721 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6781 ggcctaggct tttgcaaaaa gctagcttgg ggccaccgct cagagcacct tccaccatgg
     6841 ccacctcagc aagttcccac ttgaacaaaa acatcaagca aatgtacttg tgcctgcccc
     6901 agggtgagaa agtccaagcc atgtatatct gggttgatgg tactggagaa ggactgcgct
     6961 gcaaaacccg caccctggac tgtgagccca agtgtgtaga agagttacct gagtggaatt
     7021 ttgatggctc tagtaccttt cagtctgagg gctccaacag tgacatgtat ctcagccctg
     7081 ttgccatgtt tcgggacccc ttccgcagag atcccaacaa gctggtgttc tgtgaagttt
     7141 tcaagtacaa ccggaagcct gcagagacca atttaaggca ctcgtgtaaa cggataatgg
     7201 acatggtgag caaccagcac ccctggtttg gaatggaaca ggagtatact ctgatgggaa
     7261 cagatgggca cccttttggt tggccttcca atggctttcc tgggccccaa ggtccgtatt
     7321 actgtggtgt gggcgcagac aaagcctatg gcagggatat cgtggaggct cactaccgcg
     7381 cctgcttgta tgctggggtc aagattacag gaacaaatgc tgaggtcatg cctgcccagt
     7441 gggagttcca aataggaccc tgtgaaggaa tccgcatggg agatcatctc tgggtggccc
     7501 gtttcatctt gcatcgagta tgtgaagact ttggggtaat agcaaccttt gaccccaagc
     7561 ccattcctgg gaactggaat ggtgcaggct gccataccaa ctttagcacc aaggccatgc
     7621 gggaggagaa tggtctgaag cacatcgagg aggccatcga gaaactaagc aagcggcacc
     7681 ggtaccacat tcgagcctac gatcccaagg ggggcctgga caatgcccgt cgtctgactg
     7741 ggttccacga aacgtccaac atcaacgact tttctgctgg tgtcgccaat cgcagtgcca
     7801 gcatccgcat tccccggact gtcggccagg akaagaaagg ttactttgaa gaccgccgcc
     7861 cctctgccaa ttgtgacccc tttgcagtga cagaagccat cgtccgcaca tgccttctca
     7921 atgagactgg cgacgagccc ttccaataca aaaactaatt agactttgag tgatcttgag
     7981 cctttcctag ttcatcccac cccgccccag agagatcttt gtgaaggaac cttacttctg
     8041 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     8101 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     8161 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     8221 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     8281 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     8341 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     8401 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     8461 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     8521 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     8581 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     8641 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     8701 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     8761 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8821 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctagctt cgtgtcaagg
     8881 acggtgactg cagtgaataa taaaatgtgt gtttgtccga aatacgcgtt ttgagatttc
     8941 tgtcgccgac taaattcatg tcgcgcgata gtggtgttta tcgccgatag agatggcgat
     9001 attggaaaaa tcgatatttg aaaatatggc atattgaaaa tgtcgccgat gtgagtttct
     9061 gtgtaactga tatcgccatt tttccaaaag tgatttttgg gcatacgcga tatctggcga
     9121 tagcgcttat atcgtttacg ggggatggcg atagacgact ttggtgactt gggcgattct
     9181 gtgtgtcgca aatatcgcag tttcgatata ggtgacagac gatatgaggc tatatcgccg
     9241 atagaggcga catcaagctg gcacatggcc aatgcatatc gatctataca ttgaatcaat
     9301 attggccatt agccatatta ttcattggtt atatagcata aatcaatatt ggctattggc
     9361 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     9421 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     9481 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     9541 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     9601 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     9661 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     9721 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     9781 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     9841 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     9901 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     9961 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
//
