LOCUS       40924_29836        5214 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner mini-transposon delivery vector pMaTc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
  PUBMED    20453134
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Krol JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho, 
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 5214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13"; 
            pages: "4553-4556"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box 
            445031, Moscow, ID 83844, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             34..402
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1021..1409
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     repeat_region   1451..1478
                     /label=mariner end
                     /note="mariner end"
     CDS             1647..2843
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     repeat_region   2992..3019
                     /label=mariner end
                     /note="mariner end"
     primer_bind     3152..3168
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3178..3196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3259..4305)
                     /codon_start=1
                     /gene="tnpA"
                     /product="TnpA"
                     /label=tnpA
                     /note="mariner transposase"
                     /protein_id="ADJ00065.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     gene            complement(3259..4305)
                     /gene="tnpA"
                     /label=tnpA
     oriT            5106..5214
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg
       61 aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa
      121 aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac
      181 aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac
      241 ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg
      301 ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt
      361 ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta
      421 aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag
      481 cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg agcgcctggg
      541 tcacgtgcgc gtcacgaact gcgaggcaaa caccctgccc gctgtcatgg ccgaggtgat
      601 ggcgacccag cacggcaaca cccgttccga ggccgacaag acctatcacc tgctggttag
      661 cttccgcgcg ggagagaagc ccgacgcgga gacgttgcgc gcgattgagg accgcatctg
      721 cgctgggctt ggcttcgccg agcatcagcg cgtcagtgcc gtgcatcacg acaccgacaa
      781 cctgcacatc catatcgcca tcaacaagat tcacccgacc cgaaacacca tccatgagcc
      841 gtatcgggcc taccgcgccc tcgctgacct ctgcgcgacg ctcgaacggg actacgggct
      901 tgagcgtgac aatcacgaaa cgcggcagcg cgtttccgag aaccgcgcga acgacatgga
      961 gcggcacgcg ggcgtggaaa gcctggtcgg ctggatcccc acgatgcgtc cggcgtagag
     1021 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
     1081 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
     1141 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagcttt
     1201 catgtttgac caataaaatt aatataaatc agcaacttaa atagcttcta aggttttaag
     1261 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
     1321 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
     1381 agtgacacag gaacacttaa cggctgacat gggaattgtg ccctgtacaa gaaagcaggg
     1441 tcccgggagt aacaggttgg ctgataagtc cccggtctct agtgtacaag aaagctgggt
     1501 gatcctccac ggatcactgt attcggctgc aactttgtca tgcttgacac tttatcactg
     1561 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
     1621 agcggaggct ggtccggagg ccagacgtga aacccaacag acccctgatc gtaattctga
     1681 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
     1741 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
     1801 atgcgttgat gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     1861 ggcggccggt cttgctcgtc tcgctggccg gcgctgctgt cgactacgcc atcatggcga
     1921 cggcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     1981 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     2041 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     2101 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     2161 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     2221 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     2281 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     2341 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     2401 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     2461 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     2521 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     2581 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     2641 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     2701 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     2761 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
     2821 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca
     2881 aggcgacttc cggcaagcta tacgcgccct aggagttatg gtgggagaag aacgaactgg
     2941 tcacttctga ccgtggataa ttaattgaac tcactaaagg ggtaccgcta gagaccgggg
     3001 acttatcagc caacctgtta ctcccgggac ccagctttct tgtacagggc accggtgcgg
     3061 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     3121 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     3181 tacgactcac tatagggcga attggaggat tccggtctaa caaagaaaaa cacatttttt
     3241 tgtcaaaatt cgtttttatt attcaacata gttcccttca agagcgatac aacgattata
     3301 acgaccttcc aattttttga taccattttg gtagtactcc ttcggttttg cctcaaaata
     3361 ggcctcagtt tcggcgatca cctcttcatt gcagccaaat tttttccctg cgagcatcct
     3421 tttgaggtct gagaacaaga aaaagtcgct gggggccaga tctggagaat acggtgggtg
     3481 gggaagcaat tcgaagccca attcatgaat ttttgccatc gttctcaatg acttgtggca
     3541 cggtgcgttg tcttggtgga acaacacttt tttcttcttc atgtggggcc gttttgccgc
     3601 gatttcgacc ttcaaacgct ccaataacgc catataatag tcactgttga tggtttttcc
     3661 cttctcaaga taatcgataa aaattattcc atgcgcatcc caaaaaacag aggccattac
     3721 tttgccagcg gacttttgag tctttccacg cttcggagac ggttcaccgg tcgctgtcca
     3781 ctcagccgac tgtcgattgg actcaggagt gtagtgatgg agccatgttt catccattgt
     3841 cacatatcga cggaaaaact cgggtgtatt acgagttaac agctgcaaac accgcttaga
     3901 atcatcaaca cgtcgttgtt tttggtcaaa tgtgagctcg cgcggcaccc atttcgcaca
     3961 gagcttccgc atatccaaat attgatgaat gatatgacca acacgttcct ttgatatctt
     4021 taaggcctct gctatctcga tcaacttcat tttacggtca ttcaaaatca ttttgtggat
     4081 ttttttgatg ttttcgtcgg taaccacctc tttcgggcgt ccactgcgtt caccgtcctc
     4141 cgtgctcatt tcaccacgct tgaattttgc ataccaatca attattgttg atttccctgg
     4201 ggcagagtcc ggaaactcat tatcaagcca agtttttgct tccactgtat tttttccctt
     4261 cagaaaacag tattttatca aaacacgaaa ttcctttttt tccatatgtt tcacaataac
     4321 aaaagttgct tcacaaaaga cgctctatct cacaaactaa ttgacttaca gacgtcaaat
     4381 tttgacacga atcatttgaa ggttggtact atataaaaat aatatgcatt taatactagc
     4441 gacgccatct atgtgtcaat ccatcacact ggcggcccta gtggatcctt tttgtccggt
     4501 gttgggttga aggtgaagcc ggtcggggcc gcagcggggg ccggcttttc agccttgccc
     4561 ccctgcttcg gccgccgtgg ctccggcgtc ttgggtgccg gcgcgggttc cgcagccttg
     4621 gcctgcggtg cgggcacatc ggcgggcttg gccttgatgt gccgcctggc gtgcgagcgg
     4681 aacgtctcgt aggagaactt gaccttcccc gtttcccgca tgtgctccca aatggtgacg
     4741 agcgcatagc cggacgctaa cgccgcctcg acatccgccc tcaccgccag gaacgcaacc
     4801 gcagcctcat cacgccggcg cttcttggcc gcgcgggatt caacccactc ggccagctcg
     4861 tcggtgtagc tctttggcat cgtctctcgc ctgtcccctc agttcagtaa tttcctgcat
     4921 ttgcctgttt ccagtcggta gatattccac aaaacagcag ggaagcagcg cttttccgct
     4981 gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc ctttttcgca
     5041 cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat ccaacggcgt
     5101 cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt cttcactgtc
     5161 ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg gccg
//
