LOCUS       40924_29676        5615 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mariner mini-transposon delivery vector pMaGlow, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
  PUBMED    20453134
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Krol JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho, 
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 5615)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5615)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13"; 
            pages: "4553-4556"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box 
            445031, Moscow, ID 83844, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             34..402
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      1021..1409
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     repeat_region   1451..1478
                     /label=mariner end
                     /note="mariner end"
     CDS             complement(1558..2349)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        2733..2751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2752..2776
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2791..2813
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3232..3249
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     repeat_region   3393..3420
                     /label=mariner end
                     /note="mariner end"
     primer_bind     3553..3569
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3579..3597
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3660..4706)
                     /codon_start=1
                     /gene="tnpA"
                     /product="TnpA"
                     /label=tnpA
                     /note="mariner transposase"
                     /protein_id="ADJ00068.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     gene            complement(3660..4706)
                     /gene="tnpA"
                     /label=tnpA
     oriT            5507..5615
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg
       61 aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa
      121 aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac
      181 aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac
      241 ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg
      301 ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt
      361 ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta
      421 aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca tcaagaagag
      481 cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg agcgcctggg
      541 tcacgtgcgc gtcacgaact gcgaggcaaa caccctgccc gctgtcatgg ccgaggtgat
      601 ggcgacccag cacggcaaca cccgttccga ggccgacaag acctatcacc tgctggttag
      661 cttccgcgcg ggagagaagc ccgacgcgga gacgttgcgc gcgattgagg accgcatctg
      721 cgctgggctt ggcttcgccg agcatcagcg cgtcagtgcc gtgcatcacg acaccgacaa
      781 cctgcacatc catatcgcca tcaacaagat tcacccgacc cgaaacacca tccatgagcc
      841 gtatcgggcc taccgcgccc tcgctgacct ctgcgcgacg ctcgaacggg actacgggct
      901 tgagcgtgac aatcacgaaa cgcggcagcg cgtttccgag aaccgcgcga acgacatgga
      961 gcggcacgcg ggcgtggaaa gcctggtcgg ctggatcccc acgatgcgtc cggcgtagag
     1021 gatctgaaga tcagcagttc aacctgttga tagtacgtac taagctctca tgtttcacgt
     1081 actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa ccctcatggc
     1141 taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt actaagcttt
     1201 catgtttgac caataaaatt aatataaatc agcaacttaa atagcttcta aggttttaag
     1261 ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt aacggttgtg
     1321 gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa agcaattttg
     1381 agtgacacag gaacacttaa cggctgacat gggaattgtg ccctgtacaa gaaagcaggg
     1441 tcccgggagt aacaggttgg ctgataagtc cccggtctct agtgtacaag aaagctgggt
     1501 gatcctctac gccgagacag aacttaatga attccgcgaa ccccagagtc ccgctcagaa
     1561 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     1621 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     1681 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     1741 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     1801 atcctcgccg tcgggcatcc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     1861 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     1921 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     1981 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     2041 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     2101 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     2161 ctcgtcttgg agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     2221 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     2281 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     2341 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca
     2401 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     2461 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     2521 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     2581 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     2641 tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc ggcagcgtga
     2701 agctagcgga attgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
     2761 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
     2821 atggcgtcgt tccagtcgtt cggcatcccg ggccagctgg aagtcatcaa gaaggcgctg
     2881 gatcacgtgc gcgtcggcgt ggtcatcacc gatcccgcgc tggaagataa cccgatcgtc
     2941 tacgtgaacc agggcttcgt gcagatgacc ggctacgaga ccgaggaaat cctgggcaag
     3001 aacgcgcgct tcctccaggg gaagcacacc gatccggcgg aagtggacaa catccgcacc
     3061 gcgctgcaaa ataaagaacc ggtcaccgtg cagatccaga actacaagaa ggacggcacg
     3121 atgttctgga acgaactgaa catcgatccg atggaaatcg aggataagac gtatttcgtc
     3181 ggcatccaga acgacatcac caagcagaag gaatatgaaa agctgctcga gcaccaccac
     3241 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     3301 accgctgagc aataactagt ggtgggagaa gaacgaactg gtcacttctg accgtggata
     3361 attaattgaa ctcactaaag gggtaccgct agagaccggg gacttatcag ccaacctgtt
     3421 actcccggga cccagctttc ttgtacaggg caccggtgcg ggcctcttcg ctattacgcc
     3481 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     3541 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     3601 aattggagga ttccggtcta acaaagaaaa acacattttt ttgtcaaaat tcgtttttat
     3661 tattcaacat agttcccttc aagagcgata caacgattat aacgaccttc caattttttg
     3721 ataccatttt ggtagtactc cttcggtttt gcctcaaaat aggcctcagt ttcggcgatc
     3781 acctcttcat tgcagccaaa ttttttccct gcgagcatcc ttttgaggtc tgagaacaag
     3841 aaaaagtcgc tgggggccag atctggagaa tacggtgggt ggggaagcaa ttcgaagccc
     3901 aattcatgaa tttttgccat cgttctcaat gacttgtggc acggtgcgtt gtcttggtgg
     3961 aacaacactt ttttcttctt catgtggggc cgttttgccg cgatttcgac cttcaaacgc
     4021 tccaataacg ccatataata gtcactgttg atggtttttc ccttctcaag ataatcgata
     4081 aaaattattc catgcgcatc ccaaaaaaca gaggccatta ctttgccagc ggacttttga
     4141 gtctttccac gcttcggaga cggttcaccg gtcgctgtcc actcagccga ctgtcgattg
     4201 gactcaggag tgtagtgatg gagccatgtt tcatccattg tcacatatcg acggaaaaac
     4261 tcgggtgtat tacgagttaa cagctgcaaa caccgcttag aatcatcaac acgtcgttgt
     4321 ttttggtcaa atgtgagctc gcgcggcacc catttcgcac agagcttccg catatccaaa
     4381 tattgatgaa tgatatgacc aacacgttcc tttgatatct ttaaggcctc tgctatctcg
     4441 atcaacttca ttttacggtc attcaaaatc attttgtgga tttttttgat gttttcgtcg
     4501 gtaaccacct ctttcgggcg tccactgcgt tcaccgtcct ccgtgctcat ttcaccacgc
     4561 ttgaattttg cataccaatc aattattgtt gatttccctg gggcagagtc cggaaactca
     4621 ttatcaagcc aagtttttgc ttccactgta ttttttccct tcagaaaaca gtattttatc
     4681 aaaacacgaa attccttttt ttccatatgt ttcacaataa caaaagttgc ttcacaaaag
     4741 acgctctatc tcacaaacta attgacttac agacgtcaaa ttttgacacg aatcatttga
     4801 aggttggtac tatataaaaa taatatgcat ttaatactag cgacgccatc tatgtgtcaa
     4861 tccatcacac tggcggccct agtggatcct ttttgtccgg tgttgggttg aaggtgaagc
     4921 cggtcggggc cgcagcgggg gccggctttt cagccttgcc cccctgcttc ggccgccgtg
     4981 gctccggcgt cttgggtgcc ggcgcgggtt ccgcagcctt ggcctgcggt gcgggcacat
     5041 cggcgggctt ggccttgatg tgccgcctgg cgtgcgagcg gaacgtctcg taggagaact
     5101 tgaccttccc cgtttcccgc atgtgctccc aaatggtgac gagcgcatag ccggacgcta
     5161 acgccgcctc gacatccgcc ctcaccgcca ggaacgcaac cgcagcctca tcacgccggc
     5221 gcttcttggc cgcgcgggat tcaacccact cggccagctc gtcggtgtag ctctttggca
     5281 tcgtctctcg cctgtcccct cagttcagta atttcctgca tttgcctgtt tccagtcggt
     5341 agatattcca caaaacagca gggaagcagc gcttttccgc tgcataaccc tgcttcgggg
     5401 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc
     5461 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg
     5521 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg
     5581 tgctcaacgg gaatcctgct ctgcgaggct ggccg
//
