LOCUS       40924_32883        5721 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Retroviral expression of human SOX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 5721)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    831..855
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(875..1825)
                     /codon_start=1
                     /label=hSOX2
                     /note="Homo sapiens transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPM
                     NAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKE
                     HPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHM
                     NGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPT
                     YSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGA
                     EVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM"
     protein_bind    complement(1846..1870)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(1921..2295)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             complement(2305..2721)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(2780..3137)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     LTR             complement(3200..3793)
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     promoter        3823..3927
                     /label=AmpR promoter
     CDS             3928..4785
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4952..5540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5694..5711
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttcc tgaccttgat
      541 ctgaactttt ctattctcag ttatgtattt ttccatgcct tgcaaaatgg cgttacttaa
      601 gctagcttgc caaacctaca ggtggggtct ttcattcccc cctttttctg gagactaaat
      661 aaaatctttt attttatcgt cgaccactgt gctggcggcc gctcgagttt aaatagtaca
      721 taatggattt ccttacgcga aatacgggca gacatggcct gcccggttat tattattttt
      781 gacaccagac caactggtaa tggtagcgac cggcgctcag ctggaatatc accactttgt
      841 acaagaaagc tgggtcggcg cgcccaccct ttcacatgtg tgagaggggc agtgtgccgt
      901 taatggccgt gccgggcacc gggccgctct ggtagtgctg ggacatgtga agtctgctgg
      961 gggcggcggg ttccggcacc tcggcgccgg ggagatacat gctgatcatg tcccggaggt
     1021 ccccggcctg gcagggcgcc ctggagtggg aggaagaggt aaccacaggg gggctggagc
     1081 tggcctcgga cttgaccacc gaacccatgg agccaagagc catgccaggg gtgccctgct
     1141 gcgagtagga catgctgtag gtgggcgagc cgttcatgta ggtctgcgag ctggtcatgg
     1201 agttgtactg cagggcgctc acgtcgtagc ggtgcatggg ctgcatctgc gctgcgccgt
     1261 gcgcattgag gcccgggtgc tgcgggtagc ccagctggtc ctgcatcatg ctgtagctgc
     1321 cgttgctcca gccgttcatg tgcgcgtaac tgtccatgcg ctggttcacg cccgcgccca
     1381 ggccggcgcc caccccgacc ccgctcgcca tgctattgcc gccgggggcc agcagcccgc
     1441 cgggcagcgt gtacttatcc ttcttcatga gcgtcttggt tttccgccgg ggccggtatt
     1501 tataatccgg gtgctccttc atgtgcagcg ctcgcagccg cttagcctcg tcgatgaacg
     1561 gccgcttctc cgtctccgac aaaagtttcc actcggcgcc caggcgcttg ctgatctccg
     1621 agttgtgcat cttggggttc tcctgggcca tcttgcgccg ctgcccgcgg gaccacacca
     1681 tgaaggcatt catgggccgc ttgacgcggt ccgggctgtt tttctggttg ccgccggccg
     1741 ccgccgcggt ggagttgccg ccgccgcccc ccgaagtttg ctgcgggccc ggcggcttca
     1801 gctccgtctc catcatgttg tacatggtga agggggcggc cgcggagcct gcttttttgt
     1861 acaaacttgt gatttcccgt accaccacac tgggatcctt aattaactag ctagatccgg
     1921 cagtctagag gatggtccac ccccggggtc ggcagccttc acgtgggcgg cgtgtatcca
     1981 agctgcgatg ccgtctactt tgagggcggt gggggtggtc agcaggactg tgtaaggtcc
     2041 tttccagcga ggttctaggt tcttagtctg gtgtcggcgg acccacactg tgtcgccgac
     2101 tcggtaaggg tgaggtacca ccggtcggtc cagttgttct tggtaggctg ccgccagagg
     2161 tctccagact tcgtgctgga ctaagtagag agcctgtaag tgagcttgga gagaggggct
     2221 gttagtaact cttgtcatgt cagggtcagg gaagtttaca aggggcgggg gtgccccata
     2281 taagatctca tatggccata tgggggcgcc tagagaagga gtgagggctg gataaaggga
     2341 ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg atggaggaag
     2401 aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt caaaagccaa
     2461 ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc caggtgaaaa
     2521 gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc gcggccatcc
     2581 gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct tcttgacatc
     2641 taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg tcaaacttaa
     2701 gggagtggta acagtctggc cctaattttc agacaaatac agaaacacag tcagacagag
     2761 acaacacaga acgatgctgc agcagacaag acgcgcggcg cggcttcggt cccaaaccga
     2821 aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta ccagaaccac
     2881 atatccctcc tattaggggg gtgcaccaaa gagtccaaaa cgatcgggat ttttggactc
     2941 aggtcgggcc acaaaaacgg cccccgaagt ccctgggacg tctcccaggg ttgcggccgg
     3001 gtgttccgaa ctcgtcagtt ccaccacggg tccgccagat acagagctag ttagctaact
     3061 agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac agacacagat
     3121 aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca ggggtctccc
     3181 gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat cactcagagg
     3241 agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg
     3301 atacacgggt acccgggcga ctcagtcaat cggaggactg gcgcgccgag tgaggggttg
     3361 tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg
     3421 attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat
     3481 ctgatggttc tctagaaact gctgagggct ggaccgcatc tggggaccat ctgttcttgg
     3541 ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat attcagctgt
     3601 tccatctgtt cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg
     3661 cccatattca gctgttccat ctgttcctga ccttgatctg aacttttcta ttctcagtta
     3721 tgtatttttc catgccttgc aaaatggcgt tacttaagct agcttgccaa acctacaggt
     3781 ggggtctttc attatgcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3841 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3901 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3961 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4021 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4081 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4141 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4201 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4261 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4321 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4381 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4441 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4501 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4561 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4621 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4681 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4741 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4801 gtttactcat atatacttta gattgatttg cggccggccg caaaaggatc taggtgaaga
     4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5701 cagtgagcga ggaagcggaa g
//
