LOCUS       40924_32873        6180 bp DNA     circular SYN 13-MAY-2021
DEFINITION  Retroviral expression of human KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, 
            Yamanaka S
  TITLE     Induction of pluripotent stem cells from adult human fibroblasts by 
            defined factors.
  JOURNAL   Cell. 2007 Nov 30. 131(5):861-72.
  PUBMED    18035408
REFERENCE   2  (bases 1 to 6180)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):861-72."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             complement(120..711)
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     protein_bind    906..930
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(950..2359)
                     /codon_start=1
                     /label=hKLF4
                     /note="Homo sapiens Kruppel-like factor 4 (Klf4) gene.
                     Belongs to the relatively large family of SP1-like 
                     transcription factors and is involved in the regulation of 
                     proliferation, differentiation, apoptosis and somatic cell 
                     reprogramming."
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRL
                     PPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSL
                     THPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLL
                     YGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVL
                     KASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGA
                     GPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYP
                     SFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYT
                     KSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSR
                     SDHLALHMKRHF"
     protein_bind    complement(2380..2404)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(2455..2829)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             complement(2839..3255)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(3314..3671)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     LTR             complement(3735..4251)
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     promoter        4282..4386
                     /label=AmpR promoter
     CDS             4387..5244
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5411..5999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6153..6170
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc acccttttaa aaatgtctct
      961 tcatgtgtaa ggcgaggtgg tccgacctgg aaaatgctcg gtcgcatttt tggcactgga
     1021 acgggcggtg ccccgtgtgt ttacggtagt gcctggtcag ttcatctgag cgggcgaatt
     1081 tccatccaca gccgtcccag tcacagtggt aaggtttctc acctgtgtgg gttcgcaggt
     1141 gtgccttgag atgggaactc tttgtgtagg ttttgccgca gcccgcgtaa tcacaagtgt
     1201 gggtggcggt ccttttccgg ggccacgatc gtcttcccct ctttggcttg ggctcctctg
     1261 gcatgcagga accgggtggc atgagctctt ggtaatggag cggcgggact tgcggctgca
     1321 tctgatcggg caggaaggat gggtaattgg gccccgggtg gggatggaag ccgggaggaa
     1381 gcggcagggc agggtgacag tccctgctgc tcagcacttc ctcaagaccc agggtcgggg
     1441 tagtcctgct ggggagctgc cgccccaggg ggaagtcgtg tgcagccggc cggtggccat
     1501 tgctgagagg gggtccagcg cccaagtggg tgcacgaaga gaccgcctcc tgcttgatct
     1561 tggggcacgt gcgcggcggc ccgccgttgt agggcgccac caccaccggg tggctgccgt
     1621 cagggctgcc tttgctgacg ctgatgaccg acgggctgcc gtactcgctg ccaggggcgc
     1681 tcagcgacgc cttcagcacg aacttgccca tcagcccgcc acctggcggc tgcggctgct
     1741 gcggcggaat gtacaccggg tccaattctg gccgcaggag ctcggccacg aagccgcccg
     1801 aggggctcac gtcgttgatg tccgccaggt tgaagggagc cgtcggaggg ggagcggact
     1861 ccctgccata gaggaggcct ccgcccgtgc cgcccggcgc cacgcccggg tcgttcccgg
     1921 cccggatcgg ataggtgaag ctgcaggtgg agggcgcgct ggcagggccg ctgctcgacg
     1981 gcgacgacga agaggaggct gacgctgacg aggacacggt ggcggccact gactccggag
     2041 gatgggtcag cgaattggag agaataaagt ccaggtccag gagatcgttg aactcctcgg
     2101 tctctctccg aggtaggggc gccaggttgc taccgccgca agccgcaccg gctccgccgc
     2161 tctccaggtc tgtggccacg gtcgccgccg ccaggtcata ggggcggccg ggaagcactg
     2221 ggggaagtcg cttcatgtgg gagagctcct cccgccagcg gttattcggg gcacctgctt
     2281 gacgcagtgt cttctccctt cccgccgggc cagacgcgaa cgtggagaaa gatgggagca
     2341 gcgcgtcact gacagccatg gtgaaggggg cggccgcgga gcctgctttt ttgtacaaac
     2401 ttgtgatttc ccgtaccacc acactgggat ccttaattaa ctagctagat ccggcagtct
     2461 agaggatggt ccacccccgg ggtcggcagc cttcacgtgg gcggcgtgta tccaagctgc
     2521 gatgccgtct actttgaggg cggtgggggt ggtcagcagg actgtgtaag gtcctttcca
     2581 gcgaggttct aggttcttag tctggtgtcg gcggacccac actgtgtcgc cgactcggta
     2641 agggtgaggt accaccggtc ggtccagttg ttcttggtag gctgccgcca gaggtctcca
     2701 gacttcgtgc tggactaagt agagagcctg taagtgagct tggagagagg ggctgttagt
     2761 aactcttgtc atgtcagggt cagggaagtt tacaaggggc gggggtgccc catataagat
     2821 ctcatatggc catatggggg cgcctagaga aggagtgagg gctggataaa gggaggatcg
     2881 aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg
     2941 cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc
     3001 ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg aaaagacctt
     3061 gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt
     3121 aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga catctaccga
     3181 ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac ttaagggagt
     3241 ggtaacagtc tggccctaat tttcagacaa atacagaaac acagtcagac agagacaaca
     3301 cagaacgatg ctgcagcaga caagacgcgc ggcgcggctt cggtcccaaa ccgaaagcaa
     3361 aaattcagac ggaggcggga actgttttag gttctcgtct cctaccagaa ccacatatcc
     3421 ctcctattag gggggtgcac caaagagtcc aaaacgatcg ggatttttgg actcaggtcg
     3481 ggccacaaaa acggcccccg aagtccctgg gacgtctccc agggttgcgg ccgggtgttc
     3541 cgaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct aactagtacc
     3601 gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac agataagttg
     3661 ctggccagct tacctcccgg tggtgggtcg gtggtccctg ggcaggggtc tcccgatccc
     3721 ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca gaggagaccc
     3781 tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta ttggatacac
     3841 gggtacccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg gttgtgggct
     3901 cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga actgattggt
     3961 tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa acatctgatg
     4021 gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc ttggccctga
     4081 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     4141 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
     4201 aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca ttatgcaggt
     4261 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4321 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4381 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4441 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4501 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4561 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4621 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4681 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4741 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4801 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4861 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4921 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4981 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5041 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5101 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5161 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5221 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5281 attgatttgc ggccggccgc aaaaggatct aggtgaagat cctttttgat aatctcatga
     5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
