LOCUS       62056_17655        7575 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1094..1510
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             3855..3881
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        3920..4249
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4259..4855
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     LTR             4958..5549
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(5850..6438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6605..7462)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7463..7567)
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccaccat ggccgtgact gcctgtcagg gcttggggtt cgtggtttca ctgattggga
     1981 ttgcgggcat cattgctgcc acctgcatgg accagtggag cacccaagac ttgtacaaca
     2041 accccgtaac agctgttttc aactaccagg ggctgtggcg ctcctgtgtc cgagagagct
     2101 ctggcttcac cgagtgccgg ggctacttca ccctgctggg gctgccagcc atgctgcagg
     2161 cagtgcgagc cctgatgatc gtaggcatcg tcctgggtgc cattggcctc ctggtatcca
     2221 tctttgccct gaaatgcatc cgcattggca gcatggagga ctctgccaaa gccaacatga
     2281 cactgacctc cgggatcatg ttcattgtct caggtctttg tgcaattgct ggagtgtctg
     2341 tgtttgccaa catgctggtg actaacttct ggatgtccac agctaacatg tacaccggca
     2401 tgggtgggat ggtgcagact gttcagacca ggtacacatt tggtgcggct ctgttcgtgg
     2461 gctgggtcgc tggaggcctc acactaattg ggggtgtgat gatgtgcatc gcctgccggg
     2521 gcctggcacc agaagaaacc aactacaaag ccgtttctta tcatgcctca ggccacagtg
     2581 ttgcctacaa gcctggaggc ttcaaggcca gcactggctt tgggtccaac accaaaaaca
     2641 agaagatata cgatggaggt gcccgcacag aggacgaggt ctacaactcg aacaaagaca
     2701 gccagagtga agggactgcg cagttggaca gcattggctt cagcataatc aggaaatgca
     2761 tccatgctgt ggaaaccaga gggatcaacg agcaagggct gtatcgaatt gtgggggtca
     2821 actccagagt gcagaagttg ctgagtgtcc tgatggaccc caagactgct tctgagacag
     2881 aaacagatat ctgtgctgaa tgggagataa agaccatcac tagtgctctg aagacctacc
     2941 taagaatgct tccaggacca ctcatgatgt accagtttca aagaagtttc atcaaagcag
     3001 caaaactgga gaaccaggag tctcgggtct ctgaaatcca cagccttgtt catcggctcc
     3061 cagagaaaaa tcggcagatg ttacagctgc tcatgaacca cttggcaaat gttgctaaca
     3121 accacaagca gaatttgatg acggtggcaa accttggtgt ggtgtttgga cccactctgc
     3181 tgaggcctca ggaagaaaca gtagcagcca tcatggacat caaatttcag aacattgtca
     3241 ttgagatcct aatagaaaac cacgaaaaga tatttaacac cgtgcccgat atgcctctca
     3301 ccaatgccca gctgcacctg tctcggaaga agagcagtga ctccaagccc ccgtcctgca
     3361 gcgagaggcc cctgacgctc ttccacaccg ttcagtcaac agagaaacag gaacaaagga
     3421 acagcatcat caactccagt ttggaatctg tctcatcaaa tccaaacagc atccttaatt
     3481 ccagcagcag cttacagccc aacatgaact ccagtgaccc agacctggct gtggtcaaac
     3541 ccacccggcc caactcactc cccccgaatc caagcccaac ttcacccctc tcgccatctt
     3601 ggcccatgtt ctcggcgcca tccagcccta tgcccacctc atccacgtcc agcgactcat
     3661 cccccgtcag cacaccgttc cggaaggcaa aagccttgta tgcctgcaaa gctgaacatg
     3721 actcagaact ttcgttcaca gcaggcacgg tcttcgataa tgttcaccca tctcaggagc
     3781 ctggctggtt ggaggggact ctgaacggaa agactggcct catccctgag aattacgtgg
     3841 agttcctcct cgagtaccca tacgatgttc cagattacgc ttgagcggcc gccagcacag
     3901 tggtcgacag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3961 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4021 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4081 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4141 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4201 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat
     4261 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4321 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4381 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4441 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4501 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4561 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4621 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4681 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4741 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4801 cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg
     4861 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgacccatcg tcgacgataa
     4921 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     4981 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     5041 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     5101 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     5161 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     5221 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     5281 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     5341 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     5401 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     5461 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     5521 attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt
     5581 tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg
     5641 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5701 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5761 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5821 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5881 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5941 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6001 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6061 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6121 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6181 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6241 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6301 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6361 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6421 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6481 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6541 atccttttgc ggccggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6601 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6661 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6721 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6781 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6841 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6901 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6961 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7021 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7081 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7141 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7201 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7261 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7321 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7381 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7441 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7501 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7561 ttccgcgcac atttc
//
