LOCUS       Exported                6034 bp DNA     circular SYN 07-JUL-2025
DEFINITION  Retroviral expression of mouse Oct3/4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6034)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6034)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3870..3944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             complement(120..711)
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     protein_bind    906..930
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1092..2147)
                     /codon_start=1
                     /label=mPou5f1
                     /note="Mus musculus Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role 
                     in embryonic development and stem cell pluripotency."
                     /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDSRTWLSFQGPPGGPG
                     IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG
                     TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT
                     LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA
                     RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS
                     SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV
                     PVTALGSPMHSN"
     protein_bind    complement(2159..2183)
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    complement(2234..2608)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             complement(2618..3034)
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    complement(3093..3450)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     promoter        4136..4240
                     /label=AmpR promoter
     CDS             4241..5098
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5265..5853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6007..6024
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt tgatcaacag catcactgag cttctttccc
      961 catcccaccc cccacccctg ttgtgctttt aatccctcct cagtaaaaga atttaacccc
     1021 aaagctccag gttctcttgt ctacctccct tgccttggct cacagcatcc ccagggaggg
     1081 ctggtgcctc agtttgaatg catgggagag cccagagcag tgacgggaac agagggaaag
     1141 gcctcgccct caggaaaagg gactgagtag agtgtggtga agtgggggct tccatagcct
     1201 ggggtgccaa agtggggacc tgggggcaga ggaaaggata cagccccccc tgggaaaggt
     1261 gtccctgtag cctcatactc ttctcgttgg gaatactcaa tacttgatct tttgcccttc
     1321 tggcgccggt tacagaacca tactcgaacc acatccttct ctagcccaag ctgattggcg
     1381 atgtgagtga tctgctgtag ggagggcttc gggcacttca gaaacatggt ctccagactc
     1441 cacctcacac ggttctcaat gctagttcgc tttctcttcc gggcctgcac cagggtctcc
     1501 gatttgcata tctcctgaag gttctcattg ttgtcggctt cctccaccca cttctccagc
     1561 aggggccgca gcttacacat gttcttaagg ctgagctgca aggcctcgaa gcgacagatg
     1621 gtggtctggc tgaacacctt tccaaagaga acgcccaggg tgagccccac gtcggcctgg
     1681 gtgtacccca aggtgatcct cttctgcttc agcagcttgg caaactgttc tagctccttc
     1741 tgcagggctt tcatgtcctg ggactcctcg ggagttggtt ccaccttctc caacttcacg
     1801 gcattggggc ggtcggcaca gggctcagag gaggttccct ctgagttgct ttccactcgt
     1861 gctcctgcct ggccctcagg ctgcaaagtc tccacgccaa cttgggggac taggcccagt
     1921 ccaacctgag gtccacagta tgccatccct ccgcagaact cgtatgcggg cggacatggg
     1981 gagatcccca atacctctga gcctggtccg attccaggcc cacctggagg cccttggaag
     2041 cttagccagg ttcgagaatc cacccagccc ggctccagcc ctgctgaccc atcaccccca
     2101 cctggtgggg gtgagaaggc gaagtctgaa gccaggtgtc cagccatggg gaaggtggag
     2161 cctgcttttt tgtacaaact tgtgatttcc cgtaccacca cactgggatc cttaattaac
     2221 tagctagatc cggcagtcta gaggatggtc cacccccggg gtcggcagcc ttcacgtggg
     2281 cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc ggtgggggtg gtcagcagga
     2341 ctgtgtaagg tcctttccag cgaggttcta ggttcttagt ctggtgtcgg cggacccaca
     2401 ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg gtccagttgt tcttggtagg
     2461 ctgccgccag aggtctccag acttcgtgct ggactaagta gagagcctgt aagtgagctt
     2521 ggagagaggg gctgttagta actcttgtca tgtcagggtc agggaagttt acaaggggcg
     2581 ggggtgcccc atataagatc tcatatggcc atatgggggc gcctagagaa ggagtgaggg
     2641 ctggataaag ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg
     2701 cggatggagg aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg
     2761 ggtcaaaagc caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg
     2821 ggccaggtga aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt
     2881 ctcgcggcca tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc
     2941 tcttcttgac atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta
     3001 aggtcaaact taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca
     3061 cagtcagaca gagacaacac agaacgatgc tgcagcagac aagacgcgcg gcgcggcttc
     3121 ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg ttctcgtctc
     3181 ctaccagaac cacatatccc tcctattagg ggggtgcacc aaagagtcca aaacgatcgg
     3241 gatttttgga ctcaggtcgg gccacaaaaa cggcccccga agtccctggg acgtctccca
     3301 gggttgcggc cgggtgttcc gaactcgtca gttccaccac gggtccgcca gatacagagc
     3361 tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac tagacaatcg
     3421 gacagacaca gataagttgc tggccagctt acctcccggt ggtgggtcgg tggtccctgg
     3481 gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg acgggtagtc
     3541 aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa
     3601 gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcgcc
     3661 gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc gaacagaagc
     3721 gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg tccttggggc
     3781 accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc atctggggac
     3841 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc
     3901 catattcagc tgttccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     3961 atatcctgtt tggcccatat tcagctgttc catctgttcc tgaccttgat ctgaactttt
     4021 ctattctcag ttatgtattt ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc
     4081 caaacctaca ggtggggtct ttcattatgc aggtggcact tttcggggaa atgtgcgcgg
     4141 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4201 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4261 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4321 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4381 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4441 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4501 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4561 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4621 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4681 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4741 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4801 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4861 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4921 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4981 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     5041 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     5101 actgtcagac caagtttact catatatact ttagattgat ttgcggccgg ccgcaaaagg
     5161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5941 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6001 gagcgcagcg agtcagtgag cgaggaagcg gaag
//
