LOCUS       40924_32838        3354 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-074, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 3354)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel 
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     regulatory      156..169
                     /regulatory_class="ribosome_binding_site"
     RBS             158..169
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    177..179
                     /label=translation start site
                     /note="translation start site"
     misc_feature    complement(180..287)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(296..314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(409..430)
                     /note="MWO-rv-2; reverse sequencing primer"
     rep_origin      complement(582..1127)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      1310..1353
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1410..2030)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     terminator      complement(2283..2369)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2461..3273)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctagcccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccca attcattaaa gaggagaaag gtacgcatgg
      181 gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
      241 ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat
      301 agtgagtcgt attacgacgc gtggagtgta tactggctta ctatgttggc actgatgagg
      361 gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa
      421 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
      481 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
      541 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
      601 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
      661 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
      721 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
      781 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
      841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
      901 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
      961 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     1021 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     1081 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     1141 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     1201 aagtcagccc catacgatat aagttgtcag ctgggcagca aaacccgtac cctaggtcta
     1261 gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     1321 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctaggggg agtgggacaa
     1381 aattgctact cagctaccta gaatgcttaa gacccacttt cacatttaag ttgtttttct
     1441 aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca
     1501 aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt
     1561 tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc
     1621 acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct
     1681 aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt
     1741 gctccatcgc gatgacttag taaagcacat ctaaaacttt tagccttatt acgtaaaaaa
     1801 tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca
     1861 atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc
     1921 tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta
     1981 agcagctcta atgcgctgtt aatcacttta cttttatcta atctggacat atcaattcgg
     2041 ggcgggattt catggatatg tttctttctg cgagaaccag ccatatttaa actcttctct
     2101 caaatttatg aatctattat acagaaaaat tttcctgaaa gcaaataaat tttttatgat
     2161 ttccctcgac aattcgcgct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     2221 gtcgggaaac ctgtcgtgcc gcatgcggca gcaaaacccg taccctaggt ctagggcggc
     2281 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     2341 tctttcgact gagcctttcg ttttatttga tgcctctagg gggagtggga caaaattgaa
     2401 atcaaataat gattttattt tgaccattca aatatgtatc cgctcatgaa ttaattctta
     2461 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     2521 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     2581 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     2641 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     2701 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     2761 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     2821 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     2881 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     2941 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     3001 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     3061 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     3121 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     3181 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     3241 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     3301 agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttcc
//
