LOCUS       40924_32828        4444 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-034, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 4444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. Version: 7.2.1 
            (1) Intel
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     misc_feature    complement(155..262)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(271..289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(398..420)
                     /note="MWO-rv-1; reverse sequencing primer"
     CDS             complement(739..1686)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(1734..1956)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2400..2443
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2500..3120)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     terminator      complement(3373..3459)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3551..4363)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctagcccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccca attcggtacc gggccccccc tcgaggtcga
      181 cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc
      241 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gacgcgtttc
      301 cccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
      361 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
      421 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
      481 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
      541 ttgtcgatct gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat
      601 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg
      661 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
      721 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
      781 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact
      841 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
      901 ttttcttagt ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
      961 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     1021 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     1081 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     1141 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     1201 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     1261 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     1321 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     1381 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     1441 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     1501 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     1561 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     1621 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     1681 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     1741 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     1801 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     1861 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     1921 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     1981 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     2041 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     2101 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     2161 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     2221 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     2281 ggagtggtga atccgttagc gaggtgccgc cggggaacag ctgggcagca aaacccgtac
     2341 cctaggtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     2401 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctctaggggg
     2461 agtgggacaa aattgctact cagctaccta gaatgcttaa gacccacttt cacatttaag
     2521 ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc
     2581 ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg
     2641 tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt
     2701 aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca
     2761 taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa
     2821 atgtactttt gctccatcgc gatgacttag taaagcacat ctaaaacttt tagccttatt
     2881 acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc
     2941 taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa
     3001 tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc
     3061 gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta atctggacat
     3121 atcaattcgg ggcgggattt catggatatg tttctttctg cgagaaccag ccatatttaa
     3181 actcttctct caaatttatg aatctattat acagaaaaat tttcctgaaa gcaaataaat
     3241 tttttatgat ttccctcgac aattcgcgct aacttacatt aattgcgttg cgctcactgc
     3301 ccgctttcca gtcgggaaac ctgtcgtgcc gcatgcggca gcaaaacccg taccctaggt
     3361 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     3421 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctctagg gggagtggga
     3481 caaaattgaa atcaaataat gattttattt tgaccattca aatatgtatc cgctcatgaa
     3541 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     3601 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     3661 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     3721 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     3781 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     3841 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     3901 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     3961 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     4021 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     4081 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     4141 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     4201 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     4261 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     4321 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     4381 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
     4441 ttcc
//
