LOCUS       40924_32823        4469 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMWO-005, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Low copy expression vectors for use in Yersinia sp. and related 
            organisms
  JOURNAL   Plasmid 68 (1), 33-42 (2012)
  PUBMED    22445322
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Obrist MW, Miller VL.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Microbiology and Immunology, University of 
            North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen 
            Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE   3  (bases 1 to 4469)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Microbiology and Immunology, University of North 
            Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones, 
            CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel 
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /note="MWO-fw; forward sequencing primer"
     promoter        75..148
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     regulatory      156..169
                     /regulatory_class="ribosome_binding_site"
     RBS             158..169
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    177..179
                     /label=translation start site
                     /note="translation start site"
     misc_feature    complement(180..287)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(296..314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(423..445)
                     /note="MWO-rv-1; reverse sequencing primer"
     CDS             complement(764..1711)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(1759..1981)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2425..2468
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2525..3145)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     terminator      complement(3398..3484)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3576..4388)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctagcccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
       61 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
      121 ctgagcacat cagcaggacg cactgaccca attcattaaa gaggagaaag gtacgcatgg
      181 gtaccgggcc ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
      241 ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctccaa ttcgccctat
      301 agtgagtcgt attacgacgc gtttccccgg ctgaaagcgc tatttcttcc agaattgcca
      361 tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat
      421 aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa caagttgtct
      481 caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg ttctattagg
      541 tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag ctttgaatgc
      601 accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca
      661 tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag
      721 tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag
      781 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
      841 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
      901 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg
      961 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
     1021 cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc
     1081 gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca
     1141 aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact
     1201 taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt
     1261 ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca
     1321 gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa
     1381 ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc
     1441 ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag
     1501 ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag
     1561 tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca
     1621 cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt
     1681 catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat
     1741 cactatacca attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg
     1801 tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct
     1861 ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt
     1921 atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact
     1981 cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc
     2041 tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat
     2101 cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac
     2161 attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc
     2221 cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc
     2281 tcagggcgtt ttatggcggg tctgcggagt ggtgaatccg ttagcgaggt gccgccgggg
     2341 aacagctggg cagcaaaacc cgtaccctag gtctagggcg gcggatttgt cctactcagg
     2401 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt
     2461 cgttttattt gatgcctcta gggggagtgg gacaaaattg ctactcagct acctagaatg
     2521 cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc
     2581 cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata
     2641 atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt
     2701 gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat
     2761 tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact
     2821 gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag
     2881 cacatctaaa acttttagcc ttattacgta aaaaatcttg ccagctttcc ccttctaaag
     2941 ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc
     3001 gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt
     3061 tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca
     3121 ctttactttt atctaatctg gacatatcaa ttcggggcgg gatttcatgg atatgtttct
     3181 ttctgcgaga accagccata tttaaactct tctctcaaat ttatgaatct attatacaga
     3241 aaaattttcc tgaaagcaaa taaatttttt atgatttccc tcgacaattc gcgctaactt
     3301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccgcatg
     3361 cggcagcaaa acccgtaccc taggtctagg gcggcggatt tgtcctactc aggagagcgt
     3421 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     3481 tttgatgcct ctagggggag tgggacaaaa ttgaaatcaa ataatgattt tattttgacc
     3541 attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga
     3601 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     3661 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     3721 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     3781 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta
     3841 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     3901 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     3961 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     4021 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     4081 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     4141 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     4201 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     4261 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     4321 aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata
     4381 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac
     4441 caaaatccct taacgtgagt tttcgttcc
//
