LOCUS       V004384                12509 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004384
VERSION     V004384
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12509)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12509)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12509)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12509)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..29
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     110..126
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        230..899
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    911..966
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             968..4318
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83526.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            968..4318
                     /gene="Rep"
                     /label="Rep"
     CDS             4394..5812
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83527.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4394..5812
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5796..6599
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     protein_bind    6674..6798
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6823..6853
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6907..7563
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7908..8210
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8254..8378)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8401..8424
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             8440..8463
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             8500..8523
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             8542..8559
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      8892..9102
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    9172..9196
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9351..10496)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10798..11589)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11801..12436)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
       61 tgcggacgtt tttaatgtac tggggtggtt ttggtaccgg gccccccctc gaggtcgacg
      121 gtatcgataa gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacacacttg
      181 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
      241 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
      301 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
      361 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      421 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      481 ataacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      541 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      601 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      661 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      721 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      781 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
      841 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg
      901 tatttttaca acaattacca acaacaacaa acaacagaca acattacaat tactatttac
      961 aattacaatg gcatacacac agacagctac cacatcagct ttgctggaca ctgtccgagg
     1021 aaacaactcc ttggtcaatg atctagcaaa gcgtcgtctt tacgacacag cggttgaaga
     1081 gtttaacgct cgtgaccgca ggcccaaggt gaacttttca aaagtaataa gcgaggagca
     1141 gacgcttatt gctacccggg cgtatccaga attccaaatt acattttata acacgcaaaa
     1201 tgccgtgcat tcgcttgcag gtggattgcg atctttagaa ctggaatatc tgatgatgca
     1261 aattccctac ggatcattga cttatgacat aggcgggaat tttgcatcgc atctgttcaa
     1321 gggacgagca tatgtacact gctgcatgcc caacctggac gttcgagaca tcatgcggca
     1381 cgaaggccag aaagacagta ttgaactata cctttctagg ctagagagag gggggaaaac
     1441 agtccccaac ttccaaaagg aagcatttga cagatacgca gaaattcctg aagacgctgt
     1501 ctgtcacaat actttccaga catgcgaaca tcagccgatg cagcaatcag gcagagtgta
     1561 tgccattgcg ctacacagca tatatgacat accagccgat gagttcgggg cggcactctt
     1621 gaggaaaaat gtccatacgt gctatgccgc tttccacttc tccgagaacc tgcttcttga
     1681 agattcatgc gtcaatttgg acgaaatcaa cgcgtgtttt tcgcgcgatg gagacaagtt
     1741 gaccttttct tttgcatcag agagtactct taattactgt catagttatt ctaatattct
     1801 taagtatgtg tgcaaaactt acttcccggc ctctaataga gaggtttaca tgaaggagtt
     1861 tttagtcacc agagttaata cctggttttg taagttttct agaatagata cttttctttt
     1921 gtacaaaggt gtggcccata aaagtgtaga tagtgagcag ttttatactg caatggaaga
     1981 cgcatggcat tacaaaaaga ctcttgcaat gtgcaacagc gagagaatcc tccttgagga
     2041 ttcatcatca gtcaattact ggtttcccaa aatgagggat atggtcatcg taccattatt
     2101 cgacatttct ttggagacta gtaagaggac gcgcaaggaa gtcttagtgt ccaaggattt
     2161 cgtgttcaca gtgcttaacc acattcgaac ataccaggcg aaagctctta catacgcaaa
     2221 tgttttgtcc ttcgtcgaat cgattcgatc gagggtaatc attaacggtg tgacagcgag
     2281 gtccgaatgg gatgtggaca aatctttgtt acaatccttg tccatgacgt tttacctgca
     2341 tactaagctt gccgttctaa aggatgactt actgattagc aagtttagtc tcggttcgaa
     2401 aacggtgtgc cagcatgtgt gggatgagat ttcgctggcg tttgggaacg catttccctc
     2461 cgtgaaagag aggctcttga acaggaaact tatcagagtg gcaggcgacg cattagagat
     2521 cagggtgcct gatctatatg tgaccttcca cgacagatta gtgactgagt acaaggcctc
     2581 tgtggacatg cctgcgcttg acattaggaa gaagatggaa gaaacggaag tgatgtacaa
     2641 tgcactttca gaattatcgg tgttaaggga gtctgacaaa ttcgatgttg atgttttttc
     2701 ccagatgtgc caatctttgg aagttgaccc aatgacggca gcgaaggtta tagtcgcggt
     2761 catgagcaat gagagcggtc tgactctcac atttgaacga cctactgagg cgaatgttgc
     2821 gctagcttta caggatcaag agaaggcttc agaaggtgca ttggtagtta cctcaagaga
     2881 agttgaagaa ccgtccatga agggttcgat ggccagagga gagttacaat tagctggtct
     2941 tgctggagat catccggaat cgtcctattc taagaacgag gagatagagt ctttagagca
     3001 gtttcatatg gcgacggcag attcgttaat tcgtaagcag atgagctcga ttgtgtacac
     3061 gggtccgatt aaagttcagc aaatgaaaaa ctttatcgat agcctggtag catcactatc
     3121 tgctgcggtg tcgaatctcg tcaagatcct caaagataca gctgctattg accttgaaac
     3181 ccgtcaaaag tttggagtct tggatgttgc atctaggaag tggttaatca aaccaacggc
     3241 caagagtcat gcatggggtg ttgttgaaac ccacgcgagg aagtatcatg tggcgctttt
     3301 ggaatatgat gagcagggtg tggtgacatg cgatgattgg agaagagtag ctgttagctc
     3361 tgagtctgtt gtttattccg acatggcgaa actcagaact ctgcgcagac tgcttcgaaa
     3421 cggagaaccg catgtcagta gcgcaaaggt tgttcttgtg gacggagttc cgggctgtgg
     3481 aaaaaccaaa gaaattcttt ccagggttaa ttttgatgaa gatctaattt tagtacctgg
     3541 gaagcaagcc gcggaaatga tcagaagacg tgcgaattcc tcagggatta ttgtggccac
     3601 gaaggacaac gttaaaaccg ttgattcttt catgatgaat tttgggaaaa gcacacgctg
     3661 tcagttcaag aggttattca ttgatgaagg gttgatgttg catactggtt gtgttaattt
     3721 tcttgtggcg atgtcattgt gcgaaattgc atatgtttac ggagacacac agcagattcc
     3781 atacatcaat agagtttcag gattcccgta ccccgcccat tttgccaaat tggaagttga
     3841 cgaggtggag acacgcagaa ctactctccg ttgtccagcc gatgtcacac attatctgaa
     3901 caggagatat gagggctttg tcatgagcac ttcttcggtt aaaaagtctg tttcgcagga
     3961 gatggtcggc ggagccgccg tgatcaatcc gatctcaaaa cccttgcatg gcaagatcct
     4021 gacttttacc caatcggata aagaagctct gctttcaaga gggtattcag atgttcacac
     4081 tgtgcatgaa gtgcaaggcg agacatactc tgatgtttca ctagttaggt taacccctac
     4141 accggtctcc atcattgcag gagacagccc acatgttttg gtcgcattgt caaggcacac
     4201 ctgttcgctc aagtactaca ctgttgttat ggatccttta gttagtatca ttagagatct
     4261 agagaaactt agctcgtact tgttagatat gtataaggtc gatgcaggaa cacaatagca
     4321 attacagatt gactcggtgt tcaaaggttc caatcttttt gttgcagcgc caaagactgg
     4381 tgatatttct gatatgcagt tttactatga taagtgtctc ccaggcaaca gcaccatgat
     4441 gaataatttt gatgctgtta ccatgaggtt gactgacatt tcattgaatg tcaaagattg
     4501 catattggat atgtctaagt ctgttgctgc gcctaaggat caaatcaaac cactaatacc
     4561 tatggtacga acggcggcag aaatgccacg ccagactgga ctattggaaa atttagtggc
     4621 gatgattaaa agaaacttta acgcacccga gttgtctggc atcattgata ttgaaaatac
     4681 tgcatctttg gttgtagata agttttttga tagttatttg cttaaagaaa aaagaaaacc
     4741 aaataaaaat gtttctttgt tcagtagaga gtctctcaat agatggttag aaaagcagga
     4801 acaggtaaca ataggccagc tcgcagattt tgattttgtg gatttgccag cagttgatca
     4861 gtacagacac atgattaaag cacaacccaa acaaaagttg gacacttcaa tccaaacgga
     4921 gtacccggct ttgcagacga ttgtgtacca tcaaaaaaga tcaatgcaat atcgcccgtt
     4981 gttagtgagc ttaccaggca attactggac agtgttgatt cgagcagatt ttgttttcac
     5041 aagaaagaca ccagcgcaga ttgaggattt cttcggagat ctcgacagtc atgtgccgat
     5101 ggatgtcttg gagctggata tatcaaaata cgacaaatct cagaatgaat tccactgtgc
     5161 agtagaatac gagatctggc gaagattggg tttcgaagac ttcttgggag aagtttggaa
     5221 acaagggcat agaaagacca ccctcaagga ttataccgca ggtataaaaa cttgcatctg
     5281 gtatcaaaga aagagcgggg acgtcacgac gttcattgga aacactgtga tcattgctgc
     5341 atgtttggcc tcgatgcttc cgatggagaa aataatcaaa ggagcctttt gcggtgacga
     5401 tagtctgctg tactttccaa agggttgtga gtttccggat gtgcaacact ccgcgaatct
     5461 tatgtggaat tttgaagcaa aactgtttaa aaaacagtat ggatactttt gcggaagata
     5521 tgtaatacat cacgacagag gatgcattgt gtattacgat cccctaaagt tgatctcgaa
     5581 acttggtgct aaacacatca aggattggga acacttggag gagttcagaa ggtctctttg
     5641 tgatgttgct gtttcgttga acaattgtgc gtattacaca cagttggacg acgctgtatg
     5701 ggaggttcat aagaccgccc ctccaggttc gtttgtttat aaaagtctgg tgaagtattt
     5761 gtctgataaa gttcttttta gaagtttgtt tatagatggc tctagttgtt aaaggaaaag
     5821 tgaatatcaa tgagtttatc gacctgacaa aaatggagaa gatcttaccg tcgatgttta
     5881 cccctgtaaa gagtgttatg tgttccaaag ttgataaaat aatggttcat gagaatgagt
     5941 cattgtcagg ggtgaacctt cttaaaggag ttaagcttat tgatagtgga tacgtctgtt
     6001 tagccggttt ggtcgtcacg ggcgagtgga acttgcctga caattgcaga ggaggtgtga
     6061 gcgtgtgtct ggtggacaaa aggatggaaa gagccgacga ggccattctc ggatcttact
     6121 acacagcagc tgcaaagaaa agatttcagt tcaaggtcgt tcccaattat gctataacca
     6181 cccaggacgc gatgaaaaac gtctggcaag ttttagttaa tattagaaat gtgaagatgt
     6241 cagcgggttt ctgtccgctt tctctggagt ttgtgtcggt gtgtattgtt tatagaaata
     6301 atataaaatt aggtttgaga gagaagatta caaacgtgag agacggaggg cccatggaac
     6361 ttacagaaga agtcgttgat gagttcatgg aagatgtccc tatgtcgatc aggcttgcaa
     6421 agtttcgatc tcgaaccgga aaaaagagtg atgtccgcaa agggaaaaat agtagtagtg
     6481 atcggtcagt gccgaacaag aactatagaa atgttaagga ttttggggga atgagtttta
     6541 aaaagaataa tttaatcgat gatgattcgg aggctactgt cgccgaatcg gattcgtttt
     6601 aaatagatct tacagtatca ctactccatc tcagttcgtg ttcttgtcat taattaacaa
     6661 gaaggaatta tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     6721 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     6781 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     6841 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
     6901 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     6961 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     7021 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     7081 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     7141 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     7201 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     7261 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     7321 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     7381 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     7441 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     7501 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     7561 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     7621 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     7681 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     7741 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     7801 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     7861 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     7921 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     7981 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     8041 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     8101 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     8161 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     8221 gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     8281 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     8341 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg ataattcgaa gcttgaagga
     8401 cttgaagttc tttttcaagg tcctggatct ggagcttctt ggtctcatcc tcaatttgaa
     8461 aagggaggag gttctggagg tggatctggt ggaggatcat ggtctcatcc acagttcgaa
     8521 aagggagctg gatctaccgg tcatcatcac catcaccatt gatgacctag ggcggccgcg
     8581 gtcctgcaac ttgaggtagt caagatgcat aataaataac ggattgtgtc cgtaatcaca
     8641 cgtggtgcgt acgataacgc atagtgtttt tccctccact taaatcgaag ggttgtgtct
     8701 tggatcgcgc gggtcaaatg tatatggttc atatacatcc gcaggcacgt aataaagcga
     8761 ggggttcgaa tccccccgtt acccccggta ggggcccagg tacccggatg tgttttccgg
     8821 gctgatgagt ccgtgaggac gaaacctggc tgcaggcatg caagcttggc gtaatcatgg
     8881 tcatagccta gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt
     8941 tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt
     9001 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     9061 ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagc ccggccgggg
     9121 gatccactag cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
     9181 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     9241 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac aggagatctc
     9301 agtaaagcgc tggctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa
     9361 tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg
     9421 caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg
     9481 aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac
     9541 atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg
     9601 tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc
     9661 ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg
     9721 cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc
     9781 gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag
     9841 gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt
     9901 tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg
     9961 tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac
    10021 agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata
    10081 tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc
    10141 tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc
    10201 gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg
    10261 agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc
    10321 acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag
    10381 gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa
    10441 aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac
    10501 cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac
    10561 ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag
    10621 accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc
    10681 gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg
    10741 aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa
    10801 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    10861 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    10921 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    10981 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    11041 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    11101 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    11161 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    11221 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    11281 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    11341 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    11401 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    11461 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    11521 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    11581 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    11641 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    11701 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    11761 attttgaaac cacaattatg ggtgatgctg ccaactcgag agcgggccgg gagggttcga
    11821 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
    11881 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
    11941 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
    12001 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
    12061 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
    12121 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
    12181 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
    12241 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
    12301 ttttccgcga ggtatccaca acgccggcgg ccggccgcgg tgtctcgcac acggcttcga
    12361 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
    12421 cagcccggtg agcgtctagt ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
    12481 gagctgccgg tcggggagct gttggctgg
//
