LOCUS       V004385                13207 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004385
VERSION     V004385
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13207)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 13207)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 13207)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13207)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..29
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     110..126
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        230..899
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    911..966
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             968..4318
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83548.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            968..4318
                     /gene="Rep"
                     /label="Rep"
     CDS             4394..5812
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83549.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4394..5812
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5796..6599
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     protein_bind    6668..6787
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6817..6847
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6901..7557
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7902..8204
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8248..8372)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8380..9093
                     /label="ECFP"
                     /note="enhanced CFP"
     CDS             9154..9180
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             9184..9210
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             9217..9243
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    9503..9547
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      9590..9800
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    9870..9894
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(10049..11194)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(11496..12287)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(12499..13134)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
       61 tgcggacgtt tttaatgtac tggggtggtt ttggtaccgg gccccccctc gaggtcgacg
      121 gtatcgataa gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacacacttg
      181 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
      241 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
      301 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
      361 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      421 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      481 ataacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      541 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      601 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      661 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      721 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      781 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
      841 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg
      901 tatttttaca acaattacca acaacaacaa acaacagaca acattacaat tactatttac
      961 aattacaatg gcatacacac agacagctac cacatcagct ttgctggaca ctgtccgagg
     1021 aaacaactcc ttggtcaatg atctagcaaa gcgtcgtctt tacgacacag cggttgaaga
     1081 gtttaacgct cgtgaccgca ggcccaaggt gaacttttca aaagtaataa gcgaggagca
     1141 gacgcttatt gctacccggg cgtatccaga attccaaatt acattttata acacgcaaaa
     1201 tgccgtgcat tcgcttgcag gtggattgcg atctttagaa ctggaatatc tgatgatgca
     1261 aattccctac ggatcattga cttatgacat aggcgggaat tttgcatcgc atctgttcaa
     1321 gggacgagca tatgtacact gctgcatgcc caacctggac gttcgagaca tcatgcggca
     1381 cgaaggccag aaagacagta ttgaactata cctttctagg ctagagagag gggggaaaac
     1441 agtccccaac ttccaaaagg aagcatttga cagatacgca gaaattcctg aagacgctgt
     1501 ctgtcacaat actttccaga catgcgaaca tcagccgatg cagcaatcag gcagagtgta
     1561 tgccattgcg ctacacagca tatatgacat accagccgat gagttcgggg cggcactctt
     1621 gaggaaaaat gtccatacgt gctatgccgc tttccacttc tccgagaacc tgcttcttga
     1681 agattcatgc gtcaatttgg acgaaatcaa cgcgtgtttt tcgcgcgatg gagacaagtt
     1741 gaccttttct tttgcatcag agagtactct taattactgt catagttatt ctaatattct
     1801 taagtatgtg tgcaaaactt acttcccggc ctctaataga gaggtttaca tgaaggagtt
     1861 tttagtcacc agagttaata cctggttttg taagttttct agaatagata cttttctttt
     1921 gtacaaaggt gtggcccata aaagtgtaga tagtgagcag ttttatactg caatggaaga
     1981 cgcatggcat tacaaaaaga ctcttgcaat gtgcaacagc gagagaatcc tccttgagga
     2041 ttcatcatca gtcaattact ggtttcccaa aatgagggat atggtcatcg taccattatt
     2101 cgacatttct ttggagacta gtaagaggac gcgcaaggaa gtcttagtgt ccaaggattt
     2161 cgtgttcaca gtgcttaacc acattcgaac ataccaggcg aaagctctta catacgcaaa
     2221 tgttttgtcc ttcgtcgaat cgattcgatc gagggtaatc attaacggtg tgacagcgag
     2281 gtccgaatgg gatgtggaca aatctttgtt acaatccttg tccatgacgt tttacctgca
     2341 tactaagctt gccgttctaa aggatgactt actgattagc aagtttagtc tcggttcgaa
     2401 aacggtgtgc cagcatgtgt gggatgagat ttcgctggcg tttgggaacg catttccctc
     2461 cgtgaaagag aggctcttga acaggaaact tatcagagtg gcaggcgacg cattagagat
     2521 cagggtgcct gatctatatg tgaccttcca cgacagatta gtgactgagt acaaggcctc
     2581 tgtggacatg cctgcgcttg acattaggaa gaagatggaa gaaacggaag tgatgtacaa
     2641 tgcactttca gaattatcgg tgttaaggga gtctgacaaa ttcgatgttg atgttttttc
     2701 ccagatgtgc caatctttgg aagttgaccc aatgacggca gcgaaggtta tagtcgcggt
     2761 catgagcaat gagagcggtc tgactctcac atttgaacga cctactgagg cgaatgttgc
     2821 gctagcttta caggatcaag agaaggcttc agaaggtgca ttggtagtta cctcaagaga
     2881 agttgaagaa ccgtccatga agggttcgat ggccagagga gagttacaat tagctggtct
     2941 tgctggagat catccggaat cgtcctattc taagaacgag gagatagagt ctttagagca
     3001 gtttcatatg gcgacggcag attcgttaat tcgtaagcag atgagctcga ttgtgtacac
     3061 gggtccgatt aaagttcagc aaatgaaaaa ctttatcgat agcctggtag catcactatc
     3121 tgctgcggtg tcgaatctcg tcaagatcct caaagataca gctgctattg accttgaaac
     3181 ccgtcaaaag tttggagtct tggatgttgc atctaggaag tggttaatca aaccaacggc
     3241 caagagtcat gcatggggtg ttgttgaaac ccacgcgagg aagtatcatg tggcgctttt
     3301 ggaatatgat gagcagggtg tggtgacatg cgatgattgg agaagagtag ctgttagctc
     3361 tgagtctgtt gtttattccg acatggcgaa actcagaact ctgcgcagac tgcttcgaaa
     3421 cggagaaccg catgtcagta gcgcaaaggt tgttcttgtg gacggagttc cgggctgtgg
     3481 aaaaaccaaa gaaattcttt ccagggttaa ttttgatgaa gatctaattt tagtacctgg
     3541 gaagcaagcc gcggaaatga tcagaagacg tgcgaattcc tcagggatta ttgtggccac
     3601 gaaggacaac gttaaaaccg ttgattcttt catgatgaat tttgggaaaa gcacacgctg
     3661 tcagttcaag aggttattca ttgatgaagg gttgatgttg catactggtt gtgttaattt
     3721 tcttgtggcg atgtcattgt gcgaaattgc atatgtttac ggagacacac agcagattcc
     3781 atacatcaat agagtttcag gattcccgta ccccgcccat tttgccaaat tggaagttga
     3841 cgaggtggag acacgcagaa ctactctccg ttgtccagcc gatgtcacac attatctgaa
     3901 caggagatat gagggctttg tcatgagcac ttcttcggtt aaaaagtctg tttcgcagga
     3961 gatggtcggc ggagccgccg tgatcaatcc gatctcaaaa cccttgcatg gcaagatcct
     4021 gacttttacc caatcggata aagaagctct gctttcaaga gggtattcag atgttcacac
     4081 tgtgcatgaa gtgcaaggcg agacatactc tgatgtttca ctagttaggt taacccctac
     4141 accggtctcc atcattgcag gagacagccc acatgttttg gtcgcattgt caaggcacac
     4201 ctgttcgctc aagtactaca ctgttgttat ggatccttta gttagtatca ttagagatct
     4261 agagaaactt agctcgtact tgttagatat gtataaggtc gatgcaggaa cacaatagca
     4321 attacagatt gactcggtgt tcaaaggttc caatcttttt gttgcagcgc caaagactgg
     4381 tgatatttct gatatgcagt tttactatga taagtgtctc ccaggcaaca gcaccatgat
     4441 gaataatttt gatgctgtta ccatgaggtt gactgacatt tcattgaatg tcaaagattg
     4501 catattggat atgtctaagt ctgttgctgc gcctaaggat caaatcaaac cactaatacc
     4561 tatggtacga acggcggcag aaatgccacg ccagactgga ctattggaaa atttagtggc
     4621 gatgattaaa agaaacttta acgcacccga gttgtctggc atcattgata ttgaaaatac
     4681 tgcatctttg gttgtagata agttttttga tagttatttg cttaaagaaa aaagaaaacc
     4741 aaataaaaat gtttctttgt tcagtagaga gtctctcaat agatggttag aaaagcagga
     4801 acaggtaaca ataggccagc tcgcagattt tgattttgtg gatttgccag cagttgatca
     4861 gtacagacac atgattaaag cacaacccaa acaaaagttg gacacttcaa tccaaacgga
     4921 gtacccggct ttgcagacga ttgtgtacca tcaaaaaaga tcaatgcaat atcgcccgtt
     4981 gttagtgagc ttaccaggca attactggac agtgttgatt cgagcagatt ttgttttcac
     5041 aagaaagaca ccagcgcaga ttgaggattt cttcggagat ctcgacagtc atgtgccgat
     5101 ggatgtcttg gagctggata tatcaaaata cgacaaatct cagaatgaat tccactgtgc
     5161 agtagaatac gagatctggc gaagattggg tttcgaagac ttcttgggag aagtttggaa
     5221 acaagggcat agaaagacca ccctcaagga ttataccgca ggtataaaaa cttgcatctg
     5281 gtatcaaaga aagagcgggg acgtcacgac gttcattgga aacactgtga tcattgctgc
     5341 atgtttggcc tcgatgcttc cgatggagaa aataatcaaa ggagcctttt gcggtgacga
     5401 tagtctgctg tactttccaa agggttgtga gtttccggat gtgcaacact ccgcgaatct
     5461 tatgtggaat tttgaagcaa aactgtttaa aaaacagtat ggatactttt gcggaagata
     5521 tgtaatacat cacgacagag gatgcattgt gtattacgat cccctaaagt tgatctcgaa
     5581 acttggtgct aaacacatca aggattggga acacttggag gagttcagaa ggtctctttg
     5641 tgatgttgct gtttcgttga acaattgtgc gtattacaca cagttggacg acgctgtatg
     5701 ggaggttcat aagaccgccc ctccaggttc gtttgtttat aaaagtctgg tgaagtattt
     5761 gtctgataaa gttcttttta gaagtttgtt tatagatggc tctagttgtt aaaggaaaag
     5821 tgaatatcaa tgagtttatc gacctgacaa aaatggagaa gatcttaccg tcgatgttta
     5881 cccctgtaaa gagtgttatg tgttccaaag ttgataaaat aatggttcat gagaatgagt
     5941 cattgtcagg ggtgaacctt cttaaaggag ttaagcttat tgatagtgga tacgtctgtt
     6001 tagccggttt ggtcgtcacg ggcgagtgga acttgcctga caattgcaga ggaggtgtga
     6061 gcgtgtgtct ggtggacaaa aggatggaaa gagccgacga ggccattctc ggatcttact
     6121 acacagcagc tgcaaagaaa agatttcagt tcaaggtcgt tcccaattat gctataacca
     6181 cccaggacgc gatgaaaaac gtctggcaag ttttagttaa tattagaaat gtgaagatgt
     6241 cagcgggttt ctgtccgctt tctctggagt ttgtgtcggt gtgtattgtt tatagaaata
     6301 atataaaatt aggtttgaga gagaagatta caaacgtgag agacggaggg cccatggaac
     6361 ttacagaaga agtcgttgat gagttcatgg aagatgtccc tatgtcgatc aggcttgcaa
     6421 agtttcgatc tcgaaccgga aaaaagagtg atgtccgcaa agggaaaaat agtagtagtg
     6481 atcggtcagt gccgaacaag aactatagaa atgttaagga ttttggggga atgagtttta
     6541 aaaagaataa tttaatcgat gatgattcgg aggctactgt cgccgaatcg gattcgtttt
     6601 aaatagatct tacagtatca ctactccatc tcagttcgtg ttcttgtcat taattaacct
     6661 cgagatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     6721 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     6781 ccagtcatat tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6841 tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa
     6901 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     6961 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     7021 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     7081 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     7141 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     7201 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     7261 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     7321 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     7381 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     7441 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     7501 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     7561 acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     7621 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     7681 tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata
     7741 tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg
     7801 tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga
     7861 aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt aaggtttaca
     7921 cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca
     7981 cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct
     8041 cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg
     8101 atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg
     8161 aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc
     8221 ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagcat
     8281 tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac
     8341 gtttctcgtt cagctttctt gtacaaagtg gtgcctaggg tgagcaaggg cgaggagctg
     8401 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     8461 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     8521 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     8581 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     8641 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     8701 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     8761 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
     8821 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     8881 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     8941 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     9001 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     9061 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaaaga
     9121 tctggaggtc atagaggagg tggaagaatt ttttatcctt atgatgttcc tgattatgct
     9181 ggttatcctt atgatgttcc tgattatgct ggatcttatc cttatgatgt tcctgattat
     9241 gctgctcatg gaggatcttg atgactaggg cggccgcggt cctgcaactt gaggtagtca
     9301 agatgcataa taaataacgg attgtgtccg taatcacacg tggtgcgtac gataacgcat
     9361 agtgtttttc cctccactta aatcgaaggg ttgtgtcttg gatcgcgcgg gtcaaatgta
     9421 tatggttcat atacatccgc aggcacgtaa taaagcgagg ggttcgaatc cccccgttac
     9481 ccccggtagg ggcccaggta cccggatgtg ttttccgggc tgatgagtcc gtgaggacga
     9541 aacctggctg caggcatgca agcttggcgt aatcatggtc atagcctaga gtccgcaaaa
     9601 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     9661 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     9721 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     9781 ctaaaaccaa aatccagtga cctgcagccc ggccggggga tccactagca gattgtcgtt
     9841 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     9901 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     9961 cgtccatttg tatgtgcatg ccaaccacag gagatctcag taaagcgctg gctgaacccc
    10021 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
    10081 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
    10141 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
    10201 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
    10261 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
    10321 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
    10381 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
    10441 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
    10501 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
    10561 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
    10621 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
    10681 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
    10741 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
    10801 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
    10861 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
    10921 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
    10981 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
    11041 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
    11101 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
    11161 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
    11221 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
    11281 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
    11341 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
    11401 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
    11461 tgtcgccggc atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa
    11521 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
    11581 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
    11641 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
    11701 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
    11761 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
    11821 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
    11881 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
    11941 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
    12001 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
    12061 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
    12121 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
    12181 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    12241 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    12301 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    12361 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    12421 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg
    12481 tgatgctgcc aactcgagag cgggccggga gggttcgaga agggggggca ccccccttcg
    12541 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
    12601 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
    12661 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
    12721 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
    12781 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
    12841 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
    12901 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
    12961 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
    13021 gccggcggcc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
    13081 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtctagtgg
    13141 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    13201 tggctgg
//
