LOCUS       V004387                12349 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004387
VERSION     V004387
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Kagale S, Uzuhashi S, Wigness M, Bender T, Yang W, Borhan MH,
            Rozwadowski K.
  TITLE     TMV-Gate vectors: Gateway compatible tobacco mosaic virus based
            expression vectors for functional analysis of proteins
  JOURNAL   Sci Rep 2, 874 (2012)
   PUBMED   23166857
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   Rozwadowski K, Kagale S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2012) Molecular Genetics, Agriculture and
            Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan S7N
            0X2, Canada
REFERENCE   3  (bases 1 to 12349)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12349)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep 2,
            874 (2012)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2012) Molecular Genetics, Agriculture and Agri-Food Canada,
            107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..29
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     110..126
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        230..899
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    911..966
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             968..4318
                     /codon_start=1
                     /gene="Rep"
                     /product="replicase"
                     /label="Rep"
                     /protein_id="AFX83533.1"
                     /translation="MAYTQTATTSALLDTVRGNNSLVNDLAKRRLYDTAVEEFNARDRR
                     PKVNFSKVISEEQTLIATRAYPEFQITFYNTQNAVHSLAGGLRSLELEYLMMQIPYGSL
                     TYDIGGNFASHLFKGRAYVHCCMPNLDVRDIMRHEGQKDSIELYLSRLERGGKTVPNFQ
                     KEAFDRYAEIPEDAVCHNTFQTCEHQPMQQSGRVYAIALHSIYDIPADEFGAALLRKNV
                     HTCYAAFHFSENLLLEDSCVNLDEINACFSRDGDKLTFSFASESTLNYCHSYSNILKYV
                     CKTYFPASNREVYMKEFLVTRVNTWFCKFSRIDTFLLYKGVAHKSVDSEQFYTAMEDAW
                     HYKKTLAMCNSERILLEDSSSVNYWFPKMRDMVIVPLFDISLETSKRTRKEVLVSKDFV
                     FTVLNHIRTYQAKALTYANVLSFVESIRSRVIINGVTARSEWDVDKSLLQSLSMTFYLH
                     TKLAVLKDDLLISKFSLGSKTVCQHVWDEISLAFGNAFPSVKERLLNRKLIRVAGDALE
                     IRVPDLYVTFHDRLVTEYKASVDMPALDIRKKMEETEVMYNALSELSVLRESDKFDVDV
                     FSQMCQSLEVDPMTAAKVIVAVMSNESGLTLTFERPTEANVALALQDQEKASEGALVVT
                     SREVEEPSMKGSMARGELQLAGLAGDHPESSYSKNEEIESLEQFHMATADSLIRKQMSS
                     IVYTGPIKVQQMKNFIDSLVASLSAAVSNLVKILKDTAAIDLETRQKFGVLDVASRKWL
                     IKPTAKSHAWGVVETHARKYHVALLEYDEQGVVTCDDWRRVAVSSESVVYSDMAKLRTL
                     RRLLRNGEPHVSSAKVVLVDGVPGCGKTKEILSRVNFDEDLILVPGKQAAEMIRRRANS
                     SGIIVATKDNVKTVDSFMMNFGKSTRCQFKRLFIDEGLMLHTGCVNFLVAMSLCEIAYV
                     YGDTQQIPYINRVSGFPYPAHFAKLEVDEVETRRTTLRCPADVTHYLNRRYEGFVMSTS
                     SVKKSVSQEMVGGAAVINPISKPLHGKILTFTQSDKEALLSRGYSDVHTVHEVQGETYS
                     DVSLVRLTPTPVSIIAGDSPHVLVALSRHTCSLKYYTVVMDPLVSIIRDLEKLSSYLLD
                     MYKVDAGTQ"
     gene            968..4318
                     /gene="Rep"
                     /label="Rep"
     CDS             4394..5812
                     /codon_start=1
                     /gene="RNA pol"
                     /product="RNA polymerase"
                     /label="RNA pol"
                     /protein_id="AFX83534.1"
                     /translation="MQFYYDKCLPGNSTMMNNFDAVTMRLTDISLNVKDCILDMSKSVA
                     APKDQIKPLIPMVRTAAEMPRQTGLLENLVAMIKRNFNAPELSGIIDIENTASLVVDKF
                     FDSYLLKEKRKPNKNVSLFSRESLNRWLEKQEQVTIGQLADFDFVDLPAVDQYRHMIKA
                     QPKQKLDTSIQTEYPALQTIVYHQKRSMQYRPLLVSLPGNYWTVLIRADFVFTRKTPAQ
                     IEDFFGDLDSHVPMDVLELDISKYDKSQNEFHCAVEYEIWRRLGFEDFLGEVWKQGHRK
                     TTLKDYTAGIKTCIWYQRKSGDVTTFIGNTVIIAACLASMLPMEKIIKGAFCGDDSLLY
                     FPKGCEFPDVQHSANLMWNFEAKLFKKQYGYFCGRYVIHHDRGCIVYYDPLKLISKLGA
                     KHIKDWEHLEEFRRSLCDVAVSLNNCAYYTQLDDAVWEVHKTAPPGSFVYKSLVKYLSD
                     KVLFRSLFIDGSSC"
     gene            4394..5812
                     /gene="RNA pol"
                     /label="RNA pol"
     CDS             5796..6599
                     /gene="MP"
                     /label="Movement protein"
                     /note="Movement protein from Tobacco mosaic virus (strain
                     vulgare). Accession#: P03583"
     protein_bind    6680..6804
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6829..6859
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             6913..7569
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7914..8216
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(8260..8384)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8645..8689
                     /note="TobRV/G12a_Ribozyme: aligns with Tobacco ringspot
                     virus satellite RNA and Hammerhead ribozyme G12a"
     regulatory      8732..8942
                     /label="CaMV 35S"
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     misc_feature    9012..9036
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9191..10336)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             complement(10638..11429)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(11641..12276)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
ORIGIN
        1 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
       61 tgcggacgtt tttaatgtac tggggtggtt ttggtaccgg gccccccctc gaggtcgacg
      121 gtatcgataa gcttgatatc gaattcctgc aggtcaacat ggtggagcac gacacacttg
      181 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
      241 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
      301 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
      361 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      421 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      481 ataacatggt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      541 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      601 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      661 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      721 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      781 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
      841 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggg
      901 tatttttaca acaattacca acaacaacaa acaacagaca acattacaat tactatttac
      961 aattacaatg gcatacacac agacagctac cacatcagct ttgctggaca ctgtccgagg
     1021 aaacaactcc ttggtcaatg atctagcaaa gcgtcgtctt tacgacacag cggttgaaga
     1081 gtttaacgct cgtgaccgca ggcccaaggt gaacttttca aaagtaataa gcgaggagca
     1141 gacgcttatt gctacccggg cgtatccaga attccaaatt acattttata acacgcaaaa
     1201 tgccgtgcat tcgcttgcag gtggattgcg atctttagaa ctggaatatc tgatgatgca
     1261 aattccctac ggatcattga cttatgacat aggcgggaat tttgcatcgc atctgttcaa
     1321 gggacgagca tatgtacact gctgcatgcc caacctggac gttcgagaca tcatgcggca
     1381 cgaaggccag aaagacagta ttgaactata cctttctagg ctagagagag gggggaaaac
     1441 agtccccaac ttccaaaagg aagcatttga cagatacgca gaaattcctg aagacgctgt
     1501 ctgtcacaat actttccaga catgcgaaca tcagccgatg cagcaatcag gcagagtgta
     1561 tgccattgcg ctacacagca tatatgacat accagccgat gagttcgggg cggcactctt
     1621 gaggaaaaat gtccatacgt gctatgccgc tttccacttc tccgagaacc tgcttcttga
     1681 agattcatgc gtcaatttgg acgaaatcaa cgcgtgtttt tcgcgcgatg gagacaagtt
     1741 gaccttttct tttgcatcag agagtactct taattactgt catagttatt ctaatattct
     1801 taagtatgtg tgcaaaactt acttcccggc ctctaataga gaggtttaca tgaaggagtt
     1861 tttagtcacc agagttaata cctggttttg taagttttct agaatagata cttttctttt
     1921 gtacaaaggt gtggcccata aaagtgtaga tagtgagcag ttttatactg caatggaaga
     1981 cgcatggcat tacaaaaaga ctcttgcaat gtgcaacagc gagagaatcc tccttgagga
     2041 ttcatcatca gtcaattact ggtttcccaa aatgagggat atggtcatcg taccattatt
     2101 cgacatttct ttggagacta gtaagaggac gcgcaaggaa gtcttagtgt ccaaggattt
     2161 cgtgttcaca gtgcttaacc acattcgaac ataccaggcg aaagctctta catacgcaaa
     2221 tgttttgtcc ttcgtcgaat cgattcgatc gagggtaatc attaacggtg tgacagcgag
     2281 gtccgaatgg gatgtggaca aatctttgtt acaatccttg tccatgacgt tttacctgca
     2341 tactaagctt gccgttctaa aggatgactt actgattagc aagtttagtc tcggttcgaa
     2401 aacggtgtgc cagcatgtgt gggatgagat ttcgctggcg tttgggaacg catttccctc
     2461 cgtgaaagag aggctcttga acaggaaact tatcagagtg gcaggcgacg cattagagat
     2521 cagggtgcct gatctatatg tgaccttcca cgacagatta gtgactgagt acaaggcctc
     2581 tgtggacatg cctgcgcttg acattaggaa gaagatggaa gaaacggaag tgatgtacaa
     2641 tgcactttca gaattatcgg tgttaaggga gtctgacaaa ttcgatgttg atgttttttc
     2701 ccagatgtgc caatctttgg aagttgaccc aatgacggca gcgaaggtta tagtcgcggt
     2761 catgagcaat gagagcggtc tgactctcac atttgaacga cctactgagg cgaatgttgc
     2821 gctagcttta caggatcaag agaaggcttc agaaggtgca ttggtagtta cctcaagaga
     2881 agttgaagaa ccgtccatga agggttcgat ggccagagga gagttacaat tagctggtct
     2941 tgctggagat catccggaat cgtcctattc taagaacgag gagatagagt ctttagagca
     3001 gtttcatatg gcgacggcag attcgttaat tcgtaagcag atgagctcga ttgtgtacac
     3061 gggtccgatt aaagttcagc aaatgaaaaa ctttatcgat agcctggtag catcactatc
     3121 tgctgcggtg tcgaatctcg tcaagatcct caaagataca gctgctattg accttgaaac
     3181 ccgtcaaaag tttggagtct tggatgttgc atctaggaag tggttaatca aaccaacggc
     3241 caagagtcat gcatggggtg ttgttgaaac ccacgcgagg aagtatcatg tggcgctttt
     3301 ggaatatgat gagcagggtg tggtgacatg cgatgattgg agaagagtag ctgttagctc
     3361 tgagtctgtt gtttattccg acatggcgaa actcagaact ctgcgcagac tgcttcgaaa
     3421 cggagaaccg catgtcagta gcgcaaaggt tgttcttgtg gacggagttc cgggctgtgg
     3481 aaaaaccaaa gaaattcttt ccagggttaa ttttgatgaa gatctaattt tagtacctgg
     3541 gaagcaagcc gcggaaatga tcagaagacg tgcgaattcc tcagggatta ttgtggccac
     3601 gaaggacaac gttaaaaccg ttgattcttt catgatgaat tttgggaaaa gcacacgctg
     3661 tcagttcaag aggttattca ttgatgaagg gttgatgttg catactggtt gtgttaattt
     3721 tcttgtggcg atgtcattgt gcgaaattgc atatgtttac ggagacacac agcagattcc
     3781 atacatcaat agagtttcag gattcccgta ccccgcccat tttgccaaat tggaagttga
     3841 cgaggtggag acacgcagaa ctactctccg ttgtccagcc gatgtcacac attatctgaa
     3901 caggagatat gagggctttg tcatgagcac ttcttcggtt aaaaagtctg tttcgcagga
     3961 gatggtcggc ggagccgccg tgatcaatcc gatctcaaaa cccttgcatg gcaagatcct
     4021 gacttttacc caatcggata aagaagctct gctttcaaga gggtattcag atgttcacac
     4081 tgtgcatgaa gtgcaaggcg agacatactc tgatgtttca ctagttaggt taacccctac
     4141 accggtctcc atcattgcag gagacagccc acatgttttg gtcgcattgt caaggcacac
     4201 ctgttcgctc aagtactaca ctgttgttat ggatccttta gttagtatca ttagagatct
     4261 agagaaactt agctcgtact tgttagatat gtataaggtc gatgcaggaa cacaatagca
     4321 attacagatt gactcggtgt tcaaaggttc caatcttttt gttgcagcgc caaagactgg
     4381 tgatatttct gatatgcagt tttactatga taagtgtctc ccaggcaaca gcaccatgat
     4441 gaataatttt gatgctgtta ccatgaggtt gactgacatt tcattgaatg tcaaagattg
     4501 catattggat atgtctaagt ctgttgctgc gcctaaggat caaatcaaac cactaatacc
     4561 tatggtacga acggcggcag aaatgccacg ccagactgga ctattggaaa atttagtggc
     4621 gatgattaaa agaaacttta acgcacccga gttgtctggc atcattgata ttgaaaatac
     4681 tgcatctttg gttgtagata agttttttga tagttatttg cttaaagaaa aaagaaaacc
     4741 aaataaaaat gtttctttgt tcagtagaga gtctctcaat agatggttag aaaagcagga
     4801 acaggtaaca ataggccagc tcgcagattt tgattttgtg gatttgccag cagttgatca
     4861 gtacagacac atgattaaag cacaacccaa acaaaagttg gacacttcaa tccaaacgga
     4921 gtacccggct ttgcagacga ttgtgtacca tcaaaaaaga tcaatgcaat atcgcccgtt
     4981 gttagtgagc ttaccaggca attactggac agtgttgatt cgagcagatt ttgttttcac
     5041 aagaaagaca ccagcgcaga ttgaggattt cttcggagat ctcgacagtc atgtgccgat
     5101 ggatgtcttg gagctggata tatcaaaata cgacaaatct cagaatgaat tccactgtgc
     5161 agtagaatac gagatctggc gaagattggg tttcgaagac ttcttgggag aagtttggaa
     5221 acaagggcat agaaagacca ccctcaagga ttataccgca ggtataaaaa cttgcatctg
     5281 gtatcaaaga aagagcgggg acgtcacgac gttcattgga aacactgtga tcattgctgc
     5341 atgtttggcc tcgatgcttc cgatggagaa aataatcaaa ggagcctttt gcggtgacga
     5401 tagtctgctg tactttccaa agggttgtga gtttccggat gtgcaacact ccgcgaatct
     5461 tatgtggaat tttgaagcaa aactgtttaa aaaacagtat ggatactttt gcggaagata
     5521 tgtaatacat cacgacagag gatgcattgt gtattacgat cccctaaagt tgatctcgaa
     5581 acttggtgct aaacacatca aggattggga acacttggag gagttcagaa ggtctctttg
     5641 tgatgttgct gtttcgttga acaattgtgc gtattacaca cagttggacg acgctgtatg
     5701 ggaggttcat aagaccgccc ctccaggttc gtttgtttat aaaagtctgg tgaagtattt
     5761 gtctgataaa gttcttttta gaagtttgtt tatagatggc tctagttgtt aaaggaaaag
     5821 tgaatatcaa tgagtttatc gacctgacaa aaatggagaa gatcttaccg tcgatgttta
     5881 cccctgtaaa gagtgttatg tgttccaaag ttgataaaat aatggttcat gagaatgagt
     5941 cattgtcagg ggtgaacctt cttaaaggag ttaagcttat tgatagtgga tacgtctgtt
     6001 tagccggttt ggtcgtcacg ggcgagtgga acttgcctga caattgcaga ggaggtgtga
     6061 gcgtgtgtct ggtggacaaa aggatggaaa gagccgacga ggccattctc ggatcttact
     6121 acacagcagc tgcaaagaaa agatttcagt tcaaggtcgt tcccaattat gctataacca
     6181 cccaggacgc gatgaaaaac gtctggcaag ttttagttaa tattagaaat gtgaagatgt
     6241 cagcgggttt ctgtccgctt tctctggagt ttgtgtcggt gtgtattgtt tatagaaata
     6301 atataaaatt aggtttgaga gagaagatta caaacgtgag agacggaggg cccatggaac
     6361 ttacagaaga agtcgttgat gagttcatgg aagatgtccc tatgtcgatc aggcttgcaa
     6421 agtttcgatc tcgaaccgga aaaaagagtg atgtccgcaa agggaaaaat agtagtagtg
     6481 atcggtcagt gccgaacaag aactatagaa atgttaagga ttttggggga atgagtttta
     6541 aaaagaataa tttaatcgat gatgattcgg aggctactgt cgccgaatcg gattcgtttt
     6601 aaatagatct tacagtatca ctactccatc tcagttcgtg ttcttgtcat taattaacgg
     6661 tatcgataag cttgatatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
     6721 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
     6781 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
     6841 gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt ttcaggagct
     6901 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     6961 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     7021 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     7081 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     7141 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     7201 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     7261 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     7321 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     7381 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     7441 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     7501 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     7561 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     7621 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     7681 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     7741 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     7801 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     7861 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     7921 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     7981 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     8041 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     8101 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     8161 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     8221 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     8281 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     8341 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgatatc gaattcctgc
     8401 agtgacctag ggcggccgcg gtcctgcaac ttgaggtagt caagatgcat aataaataac
     8461 ggattgtgtc cgtaatcaca cgtggtgcgt acgataacgc atagtgtttt tccctccact
     8521 taaatcgaag ggttgtgtct tggatcgcgc gggtcaaatg tatatggttc atatacatcc
     8581 gcaggcacgt aataaagcga ggggttcgaa tccccccgtt acccccggta ggggcccagg
     8641 tacccggatg tgttttccgg gctgatgagt ccgtgaggac gaaacctggc tgcaggcatg
     8701 caagcttggc gtaatcatgg tcatagccta gagtccgcaa aaatcaccag tctctctcta
     8761 caaatctatc tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga
     8821 attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     8881 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     8941 gacctgcagc ccggccgggg gatccactag cagattgtcg tttcccgcct tcagtttaaa
     9001 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     9061 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     9121 tgccaaccac aggagatctc agtaaagcgc tggctgaacc cccagccgga actgacccca
     9181 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     9241 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     9301 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     9361 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     9421 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     9481 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     9541 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     9601 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     9661 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     9721 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     9781 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     9841 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     9901 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     9961 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
    10021 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
    10081 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
    10141 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca
    10201 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
    10261 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
    10321 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
    10381 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
    10441 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
    10501 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
    10561 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
    10621 gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
    10681 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
    10741 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
    10801 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
    10861 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
    10921 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
    10981 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
    11041 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
    11101 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
    11161 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
    11221 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
    11281 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
    11341 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
    11401 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
    11461 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
    11521 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
    11581 acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg ccaactcgag
    11641 agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc
    11701 acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa
    11761 agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc
    11821 ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct
    11881 caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc
    11941 gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg
    12001 cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc
    12061 cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc
    12121 atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg ccggccgcgg
    12181 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    12241 agcccagcgg cgagggcaac cagcccggtg agcgtctagt ggactgatgg gctgcctgta
    12301 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctgg
//
