LOCUS       62056_17625        4211 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      480..702
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             750..1697
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     primer_bind     2264..2280
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2281..2337
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2350..2366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2374..2390)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2398..2428)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2443..2464)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3144..4001)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4002..4106)
                     /label=AmpR promoter
ORIGIN
        1 gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt
       61 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa
      121 ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga
      181 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag
      241 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
      301 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
      361 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
      421 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
      481 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
      541 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
      601 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
      661 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg
      721 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
      781 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
      841 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
      901 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
      961 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
     1021 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
     1081 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc
     1141 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa
     1201 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta
     1261 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt
     1321 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc
     1381 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg
     1441 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta
     1501 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa
     1561 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc
     1621 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg
     1681 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga
     1741 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata
     1801 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt
     1861 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca
     1921 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg
     1981 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt
     2041 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca
     2101 tggcaattct ggaagaaata gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     2161 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     2221 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     2281 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
     2341 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2401 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2461 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2521 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct ttctcaatgc
     2581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3421 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     3481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     3781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4141 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4201 cttcaagaat t
//
