LOCUS       40924_32783        3675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMVAX1(c), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Du Y, Lu Y, Wang X, Qi J, Liu J, Hu Y, Li F, Wu J, Guo L, Liu J, Tao
            H, Sun W, Chen L, Cong X, Ren S, Shi J, Li J, Wang J, Huang B, Wan 
            R.
  TITLE     Highly Efficient Expression of Interleukin-2 under the Control of 
            Rabbit beta-Globin Intron II Gene Enhances Protective Immune 
            Responses of Porcine Reproductive and Respiratory Syndrome (PRRS) 
            DNA Vaccine in Pigs
  JOURNAL   PLoS ONE 9 (3), E90326 (2014)
  PUBMED    24603502
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Du Y, Wang X, Huang B, Liu J, Bi J, Wu X, Wu J, Guo L, Chen L, Sun 
            W, Wang J, Wan R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Institute of Animal Science and Veterinary 
            Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road 
            No. 8, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 3675)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "3"; pages: "E90326"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2013) Institute of Animal Science and Veterinary Medicine, 
            Shandong Academy of Agricultural Sciences, Sangyuan Road No. 8, 
            Jinan, Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          781..1353
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        1408..1426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1429..1487
                     /label=multiple cloning site
                     /note="multiple cloning site"
     polyA_signal    1505..1729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             1902..2693
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3022..3610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      661 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg
      721 cattggaacg gacccctagg cttttgcaaa aagctccgga tcgatcctga gaacttcagg
      781 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
      841 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
      901 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
      961 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     1021 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     1081 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     1141 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     1201 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa
     1261 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     1321 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1381 ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga attctgcaga
     1441 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     1501 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1561 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1621 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1681 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctactggg
     1741 cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     1801 ggaagccctg caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg
     1861 gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     1921 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     1981 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     2041 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     2101 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     2161 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     2221 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     2281 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     2341 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     2401 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     2461 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     2521 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     2581 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     2641 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaatta
     2701 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2761 accgcataca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2821 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2881 atagcacgtg ctaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     2941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctgggctttt gctggccttt
     3661 tgctcacatg ttctt
//
