LOCUS       V004391                 5641 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004391
VERSION     V004391
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3899..4291
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     regulatory      4256..4262
                     /label="derived from Mycobacterium bovis hsp60 RBS"
                     /note="derived from Mycobacterium bovis hsp60 RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             4274..5503
                     /codon_start=1
                     /product="Hsp60/VP6 chimera"
                     /label="Hsp60/VP6 chimera"
                     /note="first six amino acids of Mycobacterium bovis hsp60
                     linked to 397 amino acids of EDIM rotavirus VP6"
                     /protein_id="AAZ16497.1"
                     /translation="MAKTIADPAAEFMDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQ
                     FNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNLDANYVESARTTIDYFVDFI
                     DNVCMDEMMRESQRNGIAPQSDALRKLSGVKFRRINFNNSSEYIENWNLQNRRQRTGFT
                     FHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVAGFDYSCAINAPANIQQFEH
                     IVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYFNPVILRPNNVEVEFLLNGQV
                     INTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVTALFPNAQPFEHHATVGLTLRIDS
                     AICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWTDLITNYSPSREDNLQRVFT
                     VASIRSMLVK"
     misc_feature    4274..4291
                     /label="derived from Mycobacterium bovis hsp60 protein"
                     /note="derived from Mycobacterium bovis hsp60 protein"
     misc_feature    4310..5503
                     /note="derived from EDIM rotavirus VP6 (Choi et al., 1997)"
     terminator      5559..5605
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     2041 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     2101 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     2161 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     2221 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     2281 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     2341 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     2401 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     2461 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     2521 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     2581 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     2641 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     2701 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     2761 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     2821 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     2881 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     2941 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     3001 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     3061 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     3121 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     3181 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     3241 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     3301 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     3361 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     3421 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     3481 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     3541 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     3601 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     3661 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     3721 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     3781 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     3841 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacgg
     3901 tgaccacaac gacgcgcccg ctttgatcgg ggacgtctgc ggccgaccat ttacgggtct
     3961 tgttgtcgtt ggcggtcatg ggccgaacat actcacccgg atcggagggc cgaggacaag
     4021 gtcgaacgag gggcatgacc cggtgcgggg cttcttgcac tcggcatagg cgagtgctaa
     4081 gaataacgtt ggcactcgcg accggtgagt cgtaggtcgg gacggtgagg ccaggcccgt
     4141 cgtcgcagcg agtggcagcg aggacaactt gagccgtccg tcgcgggcac tgcgcccggc
     4201 cagcgtaagt agcggggttg ccgtcacccg gtgacccccg gtttcatccc cgatccggag
     4261 gaatcacttc gcaatggcca agacaattgc ggatccagct gcagaattca tggatgtgct
     4321 gtactctatc tcacgtacac tgaaagatgc tagggacaaa attgttgagg gtacgctgta
     4381 ctctaacgtt agtgatctta ttcaacaatt taatcaaatg ctggtcacaa tgaatggaaa
     4441 tgaatttcaa acggggggaa ttggtaactt accacttcgt aattggaatt tcgattttgg
     4501 tttattgggt actacattat taaacctgga cgcaaactat gttgaatcag ccaggaccac
     4561 aattgactac ttcgttgact tcatagataa tgtgtgcatg gatgaaatga tgcgagagtc
     4621 acaaagaaat ggaatagcgc cacaatcaga tgctctgcgg aagctgtcag gagttaagtt
     4681 cagacgaata aactttaaca actcgtcaga gtatatcgag aactggaacc ttcagaaccg
     4741 caggcaaagg acaggattca catttcacaa accgaacata tttccatact cagcatcatt
     4801 cacactaaat agatcgcagc cgcagcatga caacttaatg ggtacaatgt ggttgaatgc
     4861 tgggtcagaa atacaggttg caggattcga ctactcgtgc gcaattaatg ctccagctaa
     4921 cattcaacaa ttcgaacaca tagtgcaatt gcggagggta ttgactactg caaccattac
     4981 gctacttcca gatgcagaga ggtttagctt tccaagagtt attaactcag cagacggagc
     5041 aactacatgg tacttcaacc cagtgatatt gcgcccaaat aatgtagaag ttgagttcct
     5101 attgaacggt caggtcatca atacttacca ggccagattc ggcaccatcg ttgcaagaaa
     5161 ttttgacacc atacgccttt catttcaatt gatgagaccg ccaaacatga caccagcagt
     5221 gactgcactt ttcccaaatg ctcagccgtt cgaacaccat gcaacagtcg gactgacact
     5281 tagaattgac tcagcaattt gtgaatcagt tctcgcggat gcaagtgaaa ctatgttagc
     5341 caatgtgacg tcagtcagac aagaatacgc gataccagta ggaccagtgt ttccaccagg
     5401 aatgaattgg accgacctca ttaccaacta ctcaccatca agagaagaca acctacaacg
     5461 cgtatttaca gtagcttcca ttagaagcat gctggtaaag tgagcgaagc ttatcgatgt
     5521 cgacgtagtt aactagcgta cgatcgactg ccaggcatca aataaaacga aaggctcagt
     5581 cgaaagactg ggcctttcgt tttatctgtt gtttgtccgg ccatcatggc cgcggtgatc
     5641 a
//
