LOCUS       V004392                 6508 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004392
VERSION     V004392
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6508)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6508)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3896..4136
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4193..5164
                     /gene="fbpB"
                     /label="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B"
                     /note="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B from Mycobacterium bovis (strain BCG / Pasteur
                     1173P2). Accession#: A1KJU9"
     misc_feature    5177..6370
                     /note="derived from rotavirus EDIM VP6 of GenBank Accession
                     Number U65988"
     terminator      6426..6472
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     2041 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     2101 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     2161 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     2221 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     2281 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     2341 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     2401 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     2461 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     2521 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     2581 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     2641 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     2701 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     2761 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     2821 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     2881 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     2941 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     3001 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     3061 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     3121 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     3181 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     3241 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     3301 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     3361 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     3421 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     3481 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     3541 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     3601 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     3661 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     3721 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     3781 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     3841 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacaa
     3901 ggtcgaacga ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta
     3961 agaataacgt tggcactcgc gaccggtgag tcgtaggtcg ggacggtgag gccaggcccg
     4021 tcgtcgcagc gagtggcagc gaggacaact tgagccgtcc gtcgcgggca ctgcgcccgg
     4081 ccagcgtaag tagcggggtt gccgtcaccc ggtgaccccc ggtttcatcc ccgatcgcta
     4141 gatatccatg gatctgagca cacgacgaca tacaggacaa aggggcacag gtatgacaga
     4201 cgtgagccga aagattcgag cttggggacg ccgattgatg atcggcacgg cagcggctgt
     4261 agtccttccg ggcctggtgg ggcttgccgg cggagcggca accgcgggcg cgttctcccg
     4321 gccggggctg ccggtcgagt acctgcaggt gccgtcgccg tcgatgggcc gcgacatcaa
     4381 ggttcagttc cagagcggtg ggaacaactc acctgcggtt tatctgctcg acggcctgcg
     4441 cgcccaagac gactacaacg gctgggatat caacaccccg gcgttcgagt ggtactacca
     4501 gtcgggactg tcgatagtca tgccggtcgg cgggcagtcc agcttctaca gcgactggta
     4561 cagcccggcc tgcggtaagg ctggctgcca gacttacaag tgggaaaccc tcctgaccag
     4621 cgagctgccg caatggttgt ccgccaacag ggccgtgaag cccaccggca gcgctgcaat
     4681 cggcttgtcg atggccggct cgtcggcaat gatcttggcc gcctaccacc cccagcagtt
     4741 catctacgcc ggctcgctgt cggccctgct ggacccctct caggggatgg ggcctagcct
     4801 gatcggcctc gcgatgggtg acgccggcgg ttacaaggcc gcagacatgt ggggtccctc
     4861 gagtgacccg gcatgggagc gcaacgaccc tacgcagcag atccccaagc tggtcgcaaa
     4921 caacacccgg ctatgggttt attgcgggaa cggcaccccg aacgagttgg gcggtgccaa
     4981 catacccgcc gagttcttgg agaacttcgt tcgtagcagc aacctgaagt tccaggatgc
     5041 gtacaagccc gcgggcgggc acaacgccgt gttcaacttc ccgcccaacg gcacgcacag
     5101 ctgggagtac tggggcgctc agctcaacgc catgaagggt gacctgcaga gttcgttagg
     5161 cgcggatccg gaattcatgg atgtgctgta ctctatctca cgtacactga aagatgctag
     5221 ggacaaaatt gttgagggta cgctgtactc taacgttagt gatcttattc aacaatttaa
     5281 tcaaatgctg gtcacaatga atggaaatga atttcaaacg gggggaattg gtaacttacc
     5341 acttcgtaat tggaatttcg attttggttt attgggtact acattattaa acctggacgc
     5401 aaactatgtt gaatcagcca ggaccacaat tgactacttc gttgacttca tagataatgt
     5461 gtgcatggat gaaatgatgc gagagtcaca aagaaatgga atagcgccac aatcagatgc
     5521 cctgcggaag ctgtcaggag ttaagttcag acgaataaac tttaacaact cgtcagagta
     5581 tatcgagaac tggaaccttc agaaccgcag gcaaaggaca ggattcacat ttcacaaacc
     5641 gaacatattt ccatactcag catcattcac actaaataga tcgcagccgc agcatgacaa
     5701 cttaatgggt acaatgtggt tgaatgctgg gtcagaaata caggttgcag gattcgacta
     5761 ctcgtgcgca attaatgctc cagctaacat tcaacaattc gaacacatag tgcaattgcg
     5821 gagggtattg actactgcaa ccattacgct acttccagat gcagagaggt ttagctttcc
     5881 aagagttatt aactcagcag acggagcaac tacatggtac ttcaacccag tgatattgcg
     5941 cccaaataat gtagaagttg agttcctatt gaacggtcag gtcatcaata cttaccaggc
     6001 cagattcggc accatcgttg caagaaattt tgacaccata cgcctttcat ttcaattgat
     6061 gagaccgcca aacatgacac cagcagtgac tgcacttttc ccaaatgctc agccgttcga
     6121 acaccatgca acagtcggac tgacacttag aattgactca gcaatttgtg aatcagttct
     6181 cgcggatgca agtgaaacta tgttagccaa tgtgacgtca gtcagacaag aatacgcgat
     6241 accagtagga ccagtgtttc caccaggaat gaattggacc gacctcatta ccaactactc
     6301 accatcaaga gaagacaacc tacaacgcgt atttacagta gcttccatta gaagcatgct
     6361 ggtaaagtga gcgaagctta tcgatgtcga cgtagttaac tagcgtacga tcgactgcca
     6421 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     6481 tgtccggcca tcatggccgc ggtgatca
//
