LOCUS       V004393                 5625 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004393
VERSION     V004393
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 5625)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5625)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JUN-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2611..3723
                     /gene="33"
                     /label="Integrase"
                     /note="Integrase from Mycobacterium phage L5. Accession#:
                     P22884"
     regulatory      3896..4136
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     CDS             4201..5487
                     /codon_start=1
                     /product="19kDa antigen/VP6 chimera"
                     /label="19kDa antigen/VP6 chimera"
                     /note="21 amino acids of Mycobacterium tuberculosis 19 kDa
                     signal sequence, first 7 amino acids of 19 kDa
                     mature-protein, 3 additional amino acids and 397 amino
                     acids of rotavirus EDIM VP6"
                     /protein_id="AAZ16495.1"
                     /translation="MKRGLTVAVAGAAILVAGLSGCSSNKSTDPKMDVLYSISRTLKDA
                     RDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNWNFDFGLLGTTLLNL
                     DANYVESARTTIDYFVDFIDNVCMDEMMRESQRNGIAPQSDALRKLSGVKFRRINFNNS
                     SEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLMGTMWLNAGSEIQVA
                     GFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPRVINSADGATTWYFN
                     PVILRPNNVEVEFLLNGQVINTYQARFGTIVARNFDTIRLSFQLMRPPNMTPAVTALFP
                     NAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYAIPVGPVFPPGMNWT
                     DLITNYSPSREDNLQRVFTVASIRSMLVK"
     misc_feature    4201..4284
                     /note="derived from Mycobacterium tuberculosis 19 kDa
                     protein of GenBank Accession Number J03838"
     sig_peptide     4201..4263
                     /note="derived from Mycobacterium tuberculosis 19 kDa
                     signal peptide"
     misc_feature    4294..5487
                     /note="derived from rotavirus EDIM VP6 of GenBank Accession
                     Number U65988"
     terminator      5543..5589
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc
     2041 tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc gcagccagct
     2101 cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg gttccgaccg
     2161 ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca gctccaatct
     2221 ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt gtcagcatcg
     2281 aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca
     2341 agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg
     2401 gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg tctgccgacc
     2461 acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
     2521 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
     2581 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
     2641 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
     2701 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
     2761 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
     2821 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
     2881 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
     2941 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
     3001 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
     3061 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     3121 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     3181 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     3241 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     3301 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     3361 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     3421 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     3481 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     3541 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     3601 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     3661 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     3721 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     3781 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     3841 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagaa atctagacaa
     3901 ggtcgaacga ggggcatgac ccggtgcggg gcttcttgca ctcggcatag gcgagtgcta
     3961 agaataacgt tggcactcgc gaccggtgag tcgtaggtcg ggacggtgag gccaggcccg
     4021 tcgtcgcagc gagtggcagc gaggacaact tgagccgtcc gtcgcgggca ctgcgcccgg
     4081 ccagcgtaag tagcggggtt gccgtcaccc ggtgaccccc ggtttcatcc ccgatcgcta
     4141 gatatccatg gatctgtcct caatgccgat ggaccgctac gacaggcaaa ggagcacagg
     4201 gtgaagcgtg gactgacggt cgcggtagcc ggagccgcca ttctggtcgc aggtctttcc
     4261 ggatgttcaa gcaacaagtc cacggatccc aaaatggatg tgctgtactc tatctcacgt
     4321 acactgaaag atgctaggga caaaattgtt gagggtacgc tgtactctaa cgttagtgat
     4381 cttattcaac aatttaatca aatgctggtc acaatgaatg gaaatgaatt tcaaacgggg
     4441 ggaattggta acttaccact tcgtaattgg aatttcgatt ttggtttatt gggtactaca
     4501 ttattaaacc tggacgcaaa ctatgttgaa tcagccagga ccacaattga ctacttcgtt
     4561 gacttcatag ataatgtgtg catggatgaa atgatgcgag agtcacaaag aaatggaata
     4621 gcgccacaat cagatgctct gcggaagctg tcaggagtta agttcagacg aataaacttt
     4681 aacaactcgt cagagtatat cgagaactgg aaccttcaga accgcaggca aaggacagga
     4741 ttcacatttc acaaaccgaa catatttcca tactcagcat cattcacact aaatagatcg
     4801 cagccgcagc atgacaactt aatgggtaca atgtggttga atgctgggtc agaaatacag
     4861 gttgcaggat tcgactactc gtgcgcaatt aatgctccag ctaacattca acaattcgaa
     4921 cacatagtgc aattgcggag ggtattgact actgcaacca ttacgctact tccagatgca
     4981 gagaggttta gctttccaag agttattaac tcagcagacg gagcaactac atggtacttc
     5041 aacccagtga tattgcgccc aaataatgta gaagttgagt tcctattgaa cggtcaggtc
     5101 atcaatactt accaggccag attcggcacc atcgttgcaa gaaattttga caccatacgc
     5161 ctttcatttc aattgatgag accgccaaac atgacaccag cagtgactgc acttttccca
     5221 aatgctcagc cgttcgaaca ccatgcaaca gtcggactga cacttagaat tgactcagca
     5281 atttgtgaat cagttctcgc ggatgcaagt gaaactatgt tagccaatgt gacgtcagtc
     5341 agacaagaat acgcgatacc agtaggacca gtgtttccac caggaatgaa ttggaccgac
     5401 ctcattacca actactcacc atcaagagaa gacaacctac aacgcgtatt tacagtagct
     5461 tccattagaa gcatgctggt aaagtgagcg aagcttatcg atgtcgacgt agttaactag
     5521 cgtacgatcg actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     5581 tcgttttatc tgttgtttgt ccggccatca tggccgcggt gatca
//
