LOCUS       Exported                4494 bp DNA     circular SYN 20-NOV-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pMV261
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4494)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4494)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2035..2048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             139..951
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1285..1873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2032..3933
                     /label=Mycobacterial OriM region derived from pAL5000
                     /note="Mycobacterial OriM region derived from pAL5000"
     promoter        3965..4353
                     /label=Hsp60 promoter
                     /note="HSP60 promoter from Mycobacterium tuberculosis."
     terminator      4426..4472
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagccacc tgacgtcggg ggggggggaa agccacgttg tgtctcaaaa tctctgatgt
       61 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
      121 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
      181 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
      241 caatcaggtg cgacaatcta tcgcttgtat gggaagcccc atgcgccaga gttgtttctg
      301 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
      361 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
      421 tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct
      481 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
      541 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
      601 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
      661 gttgaacaag tctggaaaga aatgcataat cttttgccat tctcaccgga ttcagtcgtc
      721 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
      781 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
      841 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
      901 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
      961 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt
     1021 gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg
     1081 cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag
     1141 ctctcaccaa ccgtggctcc ctcactttct ggctggatga tggggcgatt caggcctggt
     1201 atgagtcagc aacaccttct tcacgaggca gacctcacta gttccatgag cgtcagaccc
     1261 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1321 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1381 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1441 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1501 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1561 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1621 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1681 agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt
     1741 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1801 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1861 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1921 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1981 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg
     2041 cacgcgtgag cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc
     2101 aggcggcagg gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag
     2161 cggcccggaa acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg
     2221 gggtgctcgg ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt
     2281 gcagttgtgg ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca
     2341 ccggtgatta atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag
     2401 ctctggctgc cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa
     2461 agccgcgcgt cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac
     2521 ctgctggtcg tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg
     2581 tcccatccgc tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg
     2641 tgggcactca acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac
     2701 atggcggcgt gcgccgaagg ccttcggcgc gccgtcgatg gcgaccgcag ttactcaggc
     2761 ctcatgacca aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc
     2821 tacacactca gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt
     2881 cagcagacca cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat
     2941 tccgtcaggt tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac
     3001 gtggacggac tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaatttccg
     3061 tgcaacgacg tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc
     3121 atttggcgtt ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag
     3181 gccacactca gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg
     3241 gcgagcacag ttgcgcggcg cgcaaagtcc gcgtcagcca tgcatggagg cattgctatg
     3301 agcgacggct acagcgacgg ctacaaccgg cagccgactg tccgcaaaaa gcggcgcgtg
     3361 accgccgccg aaggcgctcg aatcaccgga ctatccgaac gccacgtcgt ccggctcgtg
     3421 gcgcaggaac gcagcgagtg gctcgccgag caggctgcac gccgcgaacg catccgcgcc
     3481 tatcacgacg acgagggcca ctcttggccg caaacggcca aacatttcgg gctgcatctg
     3541 gacaccgtta agcgactcgg ctatcgggcg aggaaagagc gtgcggcaga acaggaagcg
     3601 gctcaaaagg cccacaacga agccgacaat ccaccgctgt tctaacgcaa ttggggagcg
     3661 ggtgtcgcgg gggttccgtg gggggttccg ttgcaacggg tcggacaggt aaaagtcctg
     3721 gtagacgcta gttttctggt ttgggccatg cctgtctcgt tgcgtgtttc gttgcgcccg
     3781 ttttgaatac cagccagacg agacggggtt ctacgaatct tggtcgatac caagccattt
     3841 ccgctgaata tcggggagct caccgccaga atcggtggtt gtggtgatgt acgtggcgaa
     3901 ctccgttgta gtgcctgtgg tggcatccgt ggcgcggccg cggtaccaga tctttaaatc
     3961 tagacggtga ccacaacgcg cccgctttga tcggggacgt ctgcggccga ccatttacgg
     4021 gtcttgttgt cgttggcggt catgggccga acatactcac ccggatcgga gggccgagga
     4081 caaggtcgaa cgaggggcat gacccggtgc ggggcttctt gcactcggca taggcgagtg
     4141 ctaagaataa cgttggcact cgcgaccggt gagtgctagg tcgggacggt gaggccaggc
     4201 ccgtcgtcgc agcgagtggc agcgaggaca acttgagccg tccgtcgcgg gcactgcgcc
     4261 cggccagcgt aagtagcggg gttgccgtca cccggtgacc cccgtttcat ccccgatccg
     4321 gaggaatcac ttcgcaatgg ccaagacaat tgcggatcca gctgcagaat tcgaagctta
     4381 tcgatgtcga cgtagttaac tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag
     4441 gctcagtcga aagactgggc ctttcgtttt atgccatcat ggccgcggtg atca
//
