LOCUS       62056_17610        7169 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1268..1856
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3951..6995
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     CDS             7026..7043
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      7087..7133
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagccaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
      181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1201 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1981 ccgctcgccg cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg
     2041 ggtgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg
     2101 gcgtgtacgg ccgccacagc ggctcttagc ggcccggaaa cgtcctcgaa acgacgcatg
     2161 tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gtttcatcat
     2221 cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat
     2281 ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggttta ttatcaagcg
     2341 tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggtcc ctggcaagcg
     2401 acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca
     2461 tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg
     2521 cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca
     2581 atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg
     2641 aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg
     2701 ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct
     2761 gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg
     2821 gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc
     2881 cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cttcccgccc
     2941 tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg
     3001 agtgccacgc gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg
     3061 acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca
     3121 tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccat gcggccatct
     3181 cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg
     3241 cgtcagccat ggaggcattg ctatgagcga cggctacagc gacggctaca gcgacggcta
     3301 caactggcag ccgactgtcc gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat
     3361 caccggacta tccgaacgcc acgtcgtccg gctcgtggcg caggaacgca gcgagtggtt
     3421 cgccgagcag gctgcacgcc gcgaacgcat ccgcgcctat cacgacgacg agggccactc
     3481 ttggccgcaa acggccaaac atttcgggct gcatctggac accgttaagc gactcggcta
     3541 tcgggcgagg aaagagcgtg cggcagaaca ggaagcggct caaaaggccc acaacgaagc
     3601 cgacaatcca ccgctgttct aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg
     3661 ggttccgttg caacgggtcg gacaggtaaa agtcctggta gacgctagtt ttctggtttg
     3721 ggccatgcct gtctcgttgc gtgtttcgtt gcgtccgttt tgaataccag ccagacgaga
     3781 cggggttcta cgaatcttgg tcgataccaa gccatttccg ctgaatatcg tggagctcac
     3841 cgccagaatc ggtggttgtg gtgatgtacg tggcgaactc cgttgtagtg cttgtggtgg
     3901 catccgtggc gcggccgcgg taccagatct ttaaatctag agggcccatg gtcgttttac
     3961 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4021 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4081 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggt accagaagcg gtgccggaaa
     4141 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     4201 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
     4261 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     4321 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
     4381 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
     4441 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga
     4501 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
     4561 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
     4621 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
     4681 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
     4741 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
     4801 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
     4861 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
     4921 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
     4981 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
     5041 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
     5101 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
     5161 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
     5221 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
     5281 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
     5341 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
     5401 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
     5461 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
     5521 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
     5581 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
     5641 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
     5701 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
     5761 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
     5821 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
     5881 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
     5941 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
     6001 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
     6061 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
     6121 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
     6181 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
     6241 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
     6301 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
     6361 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
     6421 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
     6481 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
     6541 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
     6601 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
     6661 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
     6721 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
     6781 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
     6841 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
     6901 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
     6961 gtcgctacca ttaccagttg gtctggtgtc aaaaacggat ccagctgcag aattcgaagc
     7021 ttatgcatca tcaccatcac cattgagtcg acgtagttaa ctagcgtacg atcgactgcc
     7081 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     7141 ttgtccggcc atcatggccg cggtgatca
//
