LOCUS       V004407                 8975 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004407
VERSION     V004407
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Zhou J, Olson DG, Argyros DA, Deng Y, van Gulik WM, van Dijken JP,
            Lynd LR.
  TITLE     Atypical Glycolysis in Clostridium thermocellum
  JOURNAL   Appl. Environ. Microbiol. 79 (9), 3000-3008 (2013)
   PUBMED   23435896
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Argyros A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2012) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "9"; pages:
            "3000-3008"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2012) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(294..1094)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1095..1315)
                     /label="URA3 promoter"
     misc_feature    1343..1846
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_feature    1888..2407
                     /label="similar to pyruvate phosphate dikinase"
                     /note="similar to pyruvate phosphate dikinase"
     misc_feature    2408..2929
                     /label="similar to pyruvate phosphate dikinase"
                     /note="similar to pyruvate phosphate dikinase"
     regulatory      complement(2932..3063)
                     /label="hpt terminator"
                     /note="hpt terminator"
                     /regulatory_class="terminator"
     CDS             complement(3064..3618)
                     /codon_start=1
                     /product="hypoxanthine phosporibosyl transferase"
                     /label="hypoxanthine phosporibosyl transferase"
                     /protein_id="AGE45855.1"
                     /translation="MINQIKEILVTREELKNNAKELGKRISSDYEGKELVLIGVLKGGV
                     VFFADLIREITIPIDVDFISVSSYGNSTKSSGVVRIIKDIDIDITNKHVLIVEDLVDTG
                     LTLHYLKSMFEARGPKDVKICTALDKPSRRKVDLEIDYKGITIPDKFVVGYGLDYAEKY
                     RNLPDVCVLDSSVYTDKEDMD"
     CDS             complement(3637..4284)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      complement(4265..4812)
                     /label="glyceraldehyde 3-phosphate dehydrogenase promoter"
                     /note="glyceraldehyde 3-phosphate dehydrogenase promoter"
                     /regulatory_class="promoter"
     misc_feature    4813..5361
                     /label="similar to pyruvate phosphate dikinase"
                     /note="similar to pyruvate phosphate dikinase"
     CDS             complement(5362..5940)
                     /codon_start=1
                     /product="thymidine kinase"
                     /label="thymidine kinase"
                     /protein_id="AGE45857.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     regulatory      complement(5941..6561)
                     /label="cbp promoter"
                     /note="cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(6671..7672)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(7673..8133)
                     /direction=LEFT
                     /label="Clostridium thermocellum replication origin"
                     /note="Clostridium thermocellum replication origin"
     rep_origin      complement(8364..8952)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
       61 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      181 agcgatctgt caattcgcgg ccgccgaaaa gtgggtaata actgatataa ttaaattgaa
      241 gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc
      301 tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc
      361 ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta
      421 gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa
      481 cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt
      541 tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta
      601 gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct
      661 ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc
      721 accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag
      781 tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa
      841 ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc
      901 aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
      961 agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc
     1021 atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat
     1081 gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt
     1141 aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta
     1201 agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt
     1261 caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagct
     1321 agcttatcga tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     1381 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
     1441 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
     1501 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
     1561 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
     1621 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
     1681 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
     1741 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
     1801 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatcgcgg ccgcggtacc
     1861 cggggatcct ctagagtcga cctgcagaag ccatgcaacc tggacgttaa aattgacaga
     1921 atacaaataa attataacat ttctatcatt caatgtaaat ctcaaaaggt cttaagataa
     1981 ggaagctgac tttccctatt tttagataaa ctgtttataa caacttttat taatttattt
     2041 tatatatact tggcaaggta aaaaaacagt cccagcaaaa gcgtaaaaat tcttcccccg
     2101 tcaggcaaaa aaacgtaaaa aatgcttgag attgagtcaa atatttttta atatttttgt
     2161 tattgctttt tcctgctacc aaacacttct taagctttta atattcataa aaaatatgta
     2221 tttaaaaaaa caccctacca cttttacatg atagggtgtt aatatatacc ttttgctatt
     2281 tattattttt ttattatttc tgtccttatt atttctgtcc caaatccttt gtacctttta
     2341 tttcatttac tcttgcctga gccgctgcaa gccttgcaat cggcacacgg aacggagagc
     2401 atgatactcc tccaagcagg tctctcattg atgcattgcc ttcactaaac aaatacacat
     2461 actttgccat aaaaaaacct cccatttatt tattttttct ttatgtatgt aggaaagcat
     2521 ataaacgcaa atactttcct aaataagcgc ttgaagtttg aaaaaagact tgtcaggaat
     2581 aaattcatac caactcattt aaaattataa cataataata gtatttttca ttattatttt
     2641 tgctaaaata tgcaatattc ctttttgtaa ccctcaagtt caaaaaaatt tttcttttta
     2701 gaaaaatttt cttggacaca tatttataaa ttacccaaaa aaatataatt tcatacaacg
     2761 ttttgaaagc agattaaaca tctttacaaa atcacaattt tgttatataa taacattgaa
     2821 ttaatataaa ttttcccttg ttatcgcttc atgtcaatgc ttcattgtta catcctcttt
     2881 ttcggctgcc cgtggcgact ttcactatga agacttagga tcgcaagcag cttcaactag
     2941 ttccctcctc attctttata gcaatatagc aaaacactat ctctcataca tattattata
     3001 aaatatatat tacattatat taacataacg gtattaaaaa cacaagaaac ctttgtatat
     3061 tttttagtcc atatcttctt tgtccgtata aacagacgaa tccagcacgc acacatccgg
     3121 gagatttctg tatttttccg cataatccaa tccatagccc accacaaact tatccggtat
     3181 tgtgatacct ttataatcta tttccaaatc aacctttctc cttgacggtt tgtcaagggc
     3241 ggtgcatatt tttacatctt tgggtcctct ggcttcaaac atgcttttca gataatgcag
     3301 cgtaagacct gtatccacca agtcttcaac gataaggaca tgcttgttgg ttatatctat
     3361 atcgatgtct tttattatac gcacaacccc cgatgatttg gtggaattgc cgtaacttga
     3421 caccgatatg aaatccacat caatgggtat ggttatttcc cttattaagt cggcaaaaaa
     3481 taccactcct ccttttaaca cccctatcag gacaagctct tttccttcat agtcactgga
     3541 aatcctcttt cccaactctt tagcgttgtt tttaagttcc tctctggtaa ccaaaatttc
     3601 tttaatttga tttatcatac atccttcctc cctttataaa agccagtcat taggcctatc
     3661 tgacaattcc tgaatagagt tcataaacaa tcctgcatga taaccatcac aaacagaatg
     3721 atgtacctgt aaagatagcg gtaaatatat tgaattacct ttattaatga attttcctgc
     3781 tgtaataatg ggtagaaggt aattactatt attattgata tttaagttaa acccagtaaa
     3841 tgaagtccat ggaataatag aaagagaaaa agcattttca ggtataggtg ttttgggaaa
     3901 caatttcccc gaaccattat atttctctac atcagaaagg tataaatcat aaaactcttt
     3961 gaagtcattc tttacaggag tccaaatacc agagaatgtt ttagatacac catcaaaaat
     4021 tgtataaagt ggctctaact tatcccaata acctaactct ccgtcgctat tgtaaccagt
     4081 tctaaaagct gtatttgagt ttatcaccct tgtcactaag aaaataaatg cagggtaaaa
     4141 tttatatcct tcttgtttta tgtttcggta taaaacacta atatcaattt ctgtggttat
     4201 actaaaagtc gtttgttggt tcaaataatg attaaatatc tcttttctct tccaattgtc
     4261 taaatcaatt ttattaaagt tcatcattaa tatcgcctcc tattgtaaat taaaatttaa
     4321 tttaatgcct ctattagctg ccataaagca tgaatgcagt aaaatattca tcctctggca
     4381 agtatctctt gcactatgat tcattttata ttattttatt ttcattttca atagtttaga
     4441 taaaaaataa ttaatttttt aacaaagtat ttttatcttc ctttgatgta attctttgtt
     4501 tttttgtcca ttaatattat ggacaatttt tttatttcta gtcaataatt ttaatcttta
     4561 ttaaaacctg gcaatgccag agagatacag taattattgc atataaaaga aaacagacgc
     4621 gccctgtttg tttcctgaaa aaccacctcg tcctccaaag tatccagctt tttaaaatcc
     4681 atcctcattc ccagacgaaa aagttttgac aaagcctctt ttcttgtcca tagaattgta
     4741 ctctgcacac aatattcatc agtatttcca aaactcttta gaatcttttc ctctctttcg
     4801 gaaaagaaat acactccata tcctgcatat ctctataatg tctttcaagt ttttcggcta
     4861 tctccacaaa ctgattgtat acatcaggca ttacttcttt cagctggtca attgactggg
     4921 gagttctgat acctgcaaca acgtcttctc cctgggcatt cataaggaat tcaccataaa
     4981 gcttcttttc tcccgtagcc gggttccttg taaaggcaac tcccgtaccc gaatcatttc
     5041 ccatatttcc ataaaccatt tcctgaacgt taactgcagt accccagtcg ccggggatgt
     5101 catttaatct tctatataca atagcccttg gattttccca tgaacggaaa acggctttta
     5161 cggcttccat caactgtgtt ttcggatcct gcggaaaatc aaatcccttt tctttcttga
     5221 acagctcctt gtatcttctg actacttcct ttaaattttc ggcagaaagg tcgcagtcat
     5281 tttcacagtt gttttcttct ttcacagcat caagaatagc ttcaaacttg gacttttcca
     5341 cctccatgac aacatcgctg attaagtccg cgggacttca tggcactttc tacaccttgc
     5401 ttcataagac tcctttgctc caaccattat aatgggatcg tcgtaattgg caggcttccc
     5461 atttataagc ctttgcgtgc gagtagcagg attgccacac ttcatgcata tagcagtaag
     5521 cttatcgaca aattcagcta tggccatcaa ttctggagtc ggaccaaatg gttcacctct
     5581 aaagtccatg tcaagccctg cacatatgac tcttttcccg ctatcggcaa tctcttttac
     5641 gatgtcgact atttcagaat caaaaaattg aacttcatct atagcaaata catccgtatc
     5701 ttcttcagca taagccaata tgtcagaagc ctttactatg gcaatggcgt gcatgttgtc
     5761 gccgttatga gatacgactt tgtctatgga atacctatcg tctatagccg gtttgaaaac
     5821 ctgaactttt tgcctggcaa tcttagctct ctttatcctt cttataagtt cctcactttt
     5881 tccactgaac atgggaccag ttacaacttc tatgtacccg tggtctttag gcccatacat
     5941 ctttcgtcct ccttaaaatt ttcgtttatt tgttttttat ttagtgtgcc taaaagcacc
     6001 atcttataca ttcgtacctt atatctctta tcttgtatct cttacctcat atctcttatt
     6061 attaactacc gaagtatatt ttcataacgg aatcttatga cttatttttc tgactgccgt
     6121 tttaccatac ggttaattta tacggaaaca gctaatctaa ccttgctgaa ggcatgttta
     6181 ccaaaatatt atatctactt taccatatta attcaagtgg tcattttgat attttacgcc
     6241 aaaattttta gcgttccttt acttgataat ttatccaaaa accttcattc gtcaaaacaa
     6301 ttatgatata attaatttat gatatgatta attaatattt acggataact aatcatttca
     6361 atgcaataga tcaacatgca aatcaatatc caccttactc cgaacatttt gcaccaacga
     6421 atgcccagca ctatatcccg gcaagcaata taacgtctat aattttttat gataacgata
     6481 aaattaaatt ttatgaaagg ggtaaaagca tgagaaaaat agctgtattg ttaattactt
     6541 tgacgttctt agtcacgact cttgatatgc ctcctaaatt tttatctaaa gtgaatttag
     6601 gaggcttact tgtctgcttt cttcattaga atcaatcctt ttttaaaagt caatcccgtt
     6661 tgttgaacta ctctttaata aaataatttt tccgttccca attccacatt gcaataatag
     6721 aaaatccatc ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg
     6781 tgtcatcaag gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc
     6841 ttttacggtg taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat
     6901 cggtcataaa atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta
     6961 tatccgattt atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt
     7021 ctaatttcat tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg
     7081 tattcttaaa ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat
     7141 tatctttatt atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa
     7201 tttttttata ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta
     7261 attcttcgcc atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg
     7321 gcttttgttt aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc
     7381 tcctccagtt gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc
     7441 tttcgcctgt ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt
     7501 cagccttttt aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct
     7561 tttctctcca tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt
     7621 catctgaata aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag
     7681 ttatttgttt ggtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa
     7741 gggttttcaa tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta
     7801 aaataaaaat gacgttattt ctatatgtat caagaataga aagaactcgt ttttcgctac
     7861 gctcaaaacg caaaaaaagc actcattcga gtgctttttc ttatcgctcc aaatcatgcg
     7921 attttttcct ctttgctttt ctttgctcac gaagttctcg atcacgctgc aaaacatctt
     7981 gaagcgaaaa agtattcttc ttttcttccg atcgctcatg ctgacgcacg aaaagccctc
     8041 taggcgcata ggaacaactc ctaaatgcat gtgaggggtt ttctcgtcca tgtgaacagt
     8101 cgcatacgca atattttgtt tcccatactg cattaatgaa tcggccaacg cgcggggaga
     8161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8941 aaaggatctc aagaagatcc tttgatcttt tctac
//
