LOCUS       62056_17555        4830 bp DNA     circular SYN 22-DEC-2019
DEFINITION  Expression vector pMTagBFP2-npt, complete sequence.
ACCESSION   MK948097
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Zhang J-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2019) Center for Algal Biology and Applied 
            Research, Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 
            430072, 086
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2019) Center for Algal Biology and Applied Research, 
            Institute of Hydrobiology, Donghu Nanlu, Wuhan, Hubei 430072, 086"
FEATURES             Location/Qualifiers
     source          1..4830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2308..2334
                     /label=linker
                     /note="linker"
     CDS             2338..3030
                     /label=mTagBFP2
                     /note="enhanced monomeric blue fluorescent protein (Subach
                     et al., 2011)"
     misc_feature    3031..3060
                     /label=linker
                     /note="linker"
     CDS             3067..3090
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             3127..3150
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      3161..3208
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             3449..4240
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      4296..4314
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(4436..4452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4780..4802
                     /label=pGEX_3_primer
                     /note="pGEX_3_primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc gcttgcacgc ctctgcactc gtcggtcccg gcatccgatt
     2281 cccgggagta ctggatccaa ggcctctggt ggatctggag gtagtggtgg tagtagcgag
     2341 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
     2401 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     2461 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
     2521 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
     2581 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     2641 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     2701 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
     2761 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
     2821 ctcgtgggcg ggagccatct gatcgcaaac gccaagacca catatagatc caagaaaccc
     2881 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
     2941 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
     3001 ctccctagca aactggggca caagcttaat ggttctggtg gtggtagcac tagcgtcggt
     3061 agcgcttgga gccatcccca atttgaaaaa ggtggtggca gcggcggtgg tagcggtggt
     3121 agcgcttggt ctcacccaca attcgaaaag taagcaataa ctagcataac cccttggggc
     3181 ctctaaacgg gtcttgaggg gttttttgct gaagatgcac cttgccgtag aaggctcacg
     3241 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     3301 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     3361 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     3421 cagtcgccct aaaacaaagt taaacatcat gattgaacaa gatggattgc acgcaggttc
     3481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3601 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3901 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3961 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     4021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     4081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     4141 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     4201 gcagcgcatc gccttctatc gccttcttga cgagttcttc taacccgtgg atgacctttt
     4261 gaatgacctt taatagatta tattactaat taattgggga ccctagaggt cccctttttt
     4321 attttaaaaa ttttttcaca aaacggttta caagcataaa gcttgctcaa tcaatcaccg
     4381 gatcatcagt actcccggga tctcgacgca ttcgcgaagt accgatctcc aattcactgg
     4441 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4501 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4561 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     4621 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4681 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4741 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4801 ggttttcacc gtcatcaccg aaacgcgcga
//
