LOCUS       62056_17580        2376 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    358..379
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        394..424
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    432..448
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     456..472
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        493..511
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(538..556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(857..1445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1460..2317)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agattacaaa cgtgagagac ggagggccca tggaacttac agaagaagtc gttgatgagt
       61 tcatggaaga tgtccctatg tcgatcaggc ttgcaaagtt tcgatctcga accggaaaaa
      121 agagtgatgt ccgcaaaggg aaaaatagta gtagtgatcg gtcagtgccg aacaagaact
      181 atagaaatgt taaggatttt ggaggaatga gttttaaaaa gaataattta atcgatgatg
      241 attcggaggc tactgtcgcc gaatcggatt cgttttaaat agatcttaca gtatcactac
      301 tccatctcag ttcgtgttct tgtcaccggt gaaagcgggc agtgagcgca acgcaattaa
      361 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc cggctcgtat
      421 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      481 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtaccc aattcgccct
      541 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      601 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      721 gggacgcgct cgaggtcctg caacttgagg tagtcaagat gcataataaa taacggattg
      781 tgtccgtaat cacacgtggt gcgtacgata acgcatagtg tttttccctc cacttggaga
      841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
      901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1441 ctcaagaaga tcctttttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1561 gcttaccatc tggccccagt gctgcaatga taccgcgaga gccacgctca ccggctccag
     1621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2341 gaagcattta tcagggttat tgtctcatgg tctcga
//
