LOCUS       V016604                 3373 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016604
VERSION     V016604
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    368..389
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        404..434
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    442..458
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     466..482
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        503..521
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(548..566)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(576..592)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1099..1575
                     /gene="CP"
                     /label="Capsid protein"
                     /note="Capsid protein from Tomato mosaic virus (strain L).
                     Accession#: P03576"
     rep_origin      complement(1864..2452)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2467..3324)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 atggtctcga agattacaaa cgtgagagac ggagggccca tggaacttac agaagaagtc
       61 gttgatgagt tcatggaaga tgtccctatg tcgatcaggc ttgcaaagtt tcgatctcga
      121 accggaaaaa agagtgatgt ccgcaaaggg aaaaatagta gtagtgatcg gtcagtgccg
      181 aacaagaact atagaaatgt taaggatttt ggaggaatga gttttaaaaa gaataattta
      241 atcgatgatg attcggaggc tactgtcgcc gaatcggatt cgttttaaat agatcttaca
      301 gtatcactac tccatctcag ttcgtgttct tgtcaccggt gaaagcgggc agtgagcgca
      361 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgctcc
      421 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      481 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctgggtaccc
      541 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
      601 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      661 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      721 aatggcgaat gggacgcgct cgaggtcctg caacttgagg tagtcaagat gcataataaa
      781 taacggattg tgtccgtaat cacacgtggt gcgtacgata acgcatagtg tttttccctc
      841 cacttaaatc gaagggttgt gtcttggatc gcgcgggtca aatgtatatg gttcatatac
      901 atccgcaggc acgtaataaa gcgaggggtt cggaaccaaa tcctcaaaaa gaggtccgaa
      961 aaataataat aatttaggta aggggcgttc aggcggaagg cctaaaccaa aaagttttga
     1021 tgaagttgaa aaagagtttg ataatttgat tgaagatgaa gccgagacgt cggtcgcgga
     1081 ttctgattcg tattaaatat gtcttactca atcacttctc catcgcaatt tgtgtttttg
     1141 tcatctgtat gggctgaccc tatagaattg ttaaacgttt gtacaaattc gttaggtaac
     1201 cagtttcaaa cacagcaagc aagaactact gttcaacagc agttcagcga ggtgtggaaa
     1261 cctttccctc agagcaccgt cagatttcct ggcgatgttt ataaggtgta caggtacaat
     1321 gcagttttag atcctctaat tactgcgttg ctggggtctt tcgatactag gaatagaata
     1381 atcgaagtag aaaaccagca gaatccgaca acagctgaaa cgttagatgc tacccgcagg
     1441 gtagacgacg ctacggttgc aattcggtct gctataaata atttagttaa tgaactagta
     1501 agaggtactg gactgtacaa tcaaaatact tttgaaagta tgtctgggtt ggtctggacc
     1561 tctgcacctg catcttaaat gcataggtgc tgaaatataa agtttgtgtt tctaaaacac
     1621 acgtggtacg tacgataacg tatagtgttt ttccctccac ttaaatcgaa gggtagtgtc
     1681 ttggagcgcg cggagtaaac atatatggtt catatatgtc cgtaggcacg taaaaaagcg
     1741 agggattcga attcccccgg aacccccggg ttggggccca gggtcggcat ggcatctcca
     1801 cctcctcgcg gtccgacctg ggctacttcg gtaggctaag ggggagaccg cgttgctggc
     1861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2281 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2461 tttttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2581 ccccagtgct gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat
     2641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3361 gggttattgt ctc
//
