LOCUS       V004412                 3729 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004412
VERSION     V004412
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Heap JT, Pennington OJ, Cartman ST, Minton NP.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   J. Microbiol. Methods 78 (1), 79-85 (2009)
   PUBMED   19445976
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Pennington OJ, Heap JT, Cartman ST, Minton NP.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Centre for Biomolecular Sciences, The
            University of Nottingham, University Park, Nottingham NG7 2RD,
            United Kingdom
REFERENCE   3  (bases 1 to 3729)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Microbiol. Methods"; date: "2009"; volume: "78"; issue: "1"; pages:
            "79-85"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-MAR-2009) Centre for Biomolecular Sciences, The University of
            Nottingham, University Park, Nottingham NG7 2RD, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      9..62
                     /note="terminator derived from CD0164 gene of Clostridium
                     difficile"
                     /regulatory_class="terminator"
     primer_bind     63..79
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(199..215)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      363..404
                     /note="terminator derived from fdx gene of Clostridium
                     pasteurianum"
                     /regulatory_class="terminator"
     CDS             783..1223
                     /codon_start=1
                     /gene="repL"
                     /product="plasmid replication protein"
                     /label="repL"
                     /protein_id="ACR43917.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEID"
     gene            783..1223
                     /gene="repL"
                     /label="repL"
     CDS             1373..1993
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      2173..2761
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     oriT            3082..3191
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3224..3592
                     /label="traJ"
                     /note="oriT-recognizing protein"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
      121 ctcggtaccc ggggatcctc tagagtcgac gtcacgcgtc catggagatc tcgaggcctg
      181 cagacatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcta
      361 gcataaaaat aagaagcctg catttgcagg cttcttattt ttatggcgcg ccgcattcac
      421 ttcttttcta tataaatatg agcgaagcga ataagcgtcg gaaaagcagc aaaaagtttc
      481 ctttttgctg ttggagcatg ggggttcagg gggtgcagta tctgacgtca atgccgagcg
      541 aaagcgagcc gaagggtagc atttacgtta gataaccccc tgatatgctc cgacgcttta
      601 tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga taaggtttat
      661 aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt aacaaatgtt
      721 cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg agtgagaaaa
      781 agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata gacgaagaaa
      841 gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt aacttcgtaa
      901 aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa aaacttaaaa
      961 tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata gctacaacaa
     1021 gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca cttaaaatct
     1081 tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac cctgaactac
     1141 taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg aactttgagc
     1201 aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg ggaggtcaat
     1261 ctatgaaatg cgattaaggg ccggccagtg ggcaagttga aaaattcaca aaaatgtggt
     1321 ataatatctt tgttcattag agcgataaac ttgaatttga gagggaactt agatggtatt
     1381 tgaaaaaatt gataaaaata gttggaacag aaaagagtat tttgaccact actttgcaag
     1441 tgtaccttgt acctacagca tgaccgttaa agtggatatc acacaaataa aggaaaaggg
     1501 aatgaaacta tatcctgcaa tgctttatta tattgcaatg attgtaaacc gccattcaga
     1561 gtttaggacg gcaatcaatc aagatggtga attggggata tatgatgaga tgataccaag
     1621 ctatacaata tttcacaatg atactgaaac attttccagc ctttggactg agtgtaagtc
     1681 tgactttaaa tcatttttag cagattatga aagtgatacg caacggtatg gaaacaatca
     1741 tagaatggaa ggaaagccaa atgctccgga aaacattttt aatgtatcta tgataccgtg
     1801 gtcaaccttc gatggcttta atctgaattt gcagaaagga tatgattatt tgattcctat
     1861 ttttactatg gggaaatatt ataaagaaga taacaaaatt atacttcctt tggcaattca
     1921 agttcatcac gcagtatgtg acggatttca catttgccgt tttgtaaacg aattgcagga
     1981 attgataaat agttaacttc aggtttgtct gtaactaaaa acaagtattt aagcaaaaac
     2041 atcgtagaaa tacggtgttt tttgttaccc taagtttaaa ctcctttttg ataatctcat
     2101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2281 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2941 agagcgccca atacgcaggg ccccctgctt cggggtcatt atagcgattt tttcggtata
     3001 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
     3061 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
     3121 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
     3181 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa
     3241 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc
     3301 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg
     3361 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat
     3421 caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg
     3481 cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca
     3541 ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt
     3601 ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca
     3661 tcaagaagag cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg
     3721 atcgggccc
//
